Background: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different... more Background: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different drugs that have different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading quercetin (Que) and valproic acid (VPA) into chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of chitosan NPs loaded with a single (Que or VPA) and dual drug (Que and VPA), the characterization of the NPs, the conducting of in vitro antioxidant activity studies, and the analysis of the cytotoxicity and antioxidant activity of the NPs in human neuroblastoma SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H 2 O 2 -induced cell damage were, in order, 96 µg/mL of Que-loaded chitosan NP (77.30%, 48 h), 2 µg/mL of VPA-loaded chitosan NP (70.06%, 24 h), 96 µg/mL of blank chitosan NP (68.31%, 48 h), and 2 µg/mL of Que-and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm for developing drugloaded NPs with a uniform and homogeneous distribution of drugs into NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed. The capability of chitosan NPs developed at the nanometer scale to sustain cell viability in SH-SY5Y cell lines implies the potential of intranasal administration of chitosan NPs for future studies, offering protective effects in central nervous system diseases.
Objective: Diabetes is a disease that occurs due to pancreatic β cells failing to produce enough ... more Objective: Diabetes is a disease that occurs due to pancreatic β cells failing to produce enough insulin or the inability to use the produced insulin effectively in the body. According to the data of the IDF diabetes atlas, it has been reported that there are 537 million diabetic patients aged 20-79 worldwide in 2021 and this number is expected to reach 643 million in 2030 and 783 million in 2045. To control diabetes at such a severe level, scientists are trying to find various treatment methods. One of them is natural treatments. In this study, the affinity values of quercetin, gallic acid, oleanolic acid and ursolic acid, which have antidiabetic properties, to diabetes-related renin, cathepsin-d, T-PA, leptin, MASP-2, FABP4 proteins were investigated. Methods: For molecular docking analysis, unwanted residues and chains were deleted from the proteins with UCSF Chimera 1.15 software and prepared for docking by adding polar hydrogen atoms. Next, quercetin, gallic acid, oleanolic aci...
Objective: According to studies conducted in recent years, pesticides can lead to the development... more Objective: According to studies conducted in recent years, pesticides can lead to the development of cancer, Parkinson's disease, Alzheimer's disease, reproductive system disorders, and birth defects. The aim of this study is to examine the DNA affinity levels of Alpha-cypermethrin, Malathion, Quinclorac, and Roundup (Glyphosate) plant protection products and to discuss them in the light of the literature. Methods: Docking results between ligand and receptor were detected using Hex 8.0.0 software. Preparation of the receptor and ligand for docking was done with UCSF Chimera 1.15 software. Docking visualizations were made with BIOVIA Discovery Studio and PyMol software. While the interaction images of pesticides with DNA were detected with BIOVIA Discovery Studio software, DNA binding images were detected with PyMol software. Results: In our study, the affinity levels of the plant protection products frequently used in the Çanakkale region were determined as Alpha Cypermethri...
In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graph... more In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graphite electrode (UTGE). The cyclic voltammetry (CV) technique was used to determine the optimum conditions and the effect of pH on the electrochemical oxidation of NAP. Acetate buffer (pH 4.50) was selected as the support electrolyte due to obtaining the highest electronic signal increase during oxidation of NAP at UTGE. The differential pulse voltammetry (DPV) technique was performed for electrochemical determination of NAP. In the optimum conditions, the limits of detection (LOD) and quantification (LOQ) were determined to be 8.66 10-8 M and 2.88 10-7 M. In addition, the amount of NAP was determined in drug tablets. The recovery studies of NAP from the drug tablet were completed in order to check the accuracy and precision of the applied voltammetric method. Furthermore, the determination of NAP was performed with the high-performance liquid chromatography (HPLC) method. These two methods were compared in terms of accuracy, precision and recovery studies.
In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graph... more In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graphite electrode (UTGE). The cyclic voltammetry (CV) technique was used to determine the optimum conditions and the effect of pH on the electrochemical oxidation of NAP. Acetate buffer (pH 4.50) was selected as the support electrolyte due to obtaining the highest electronic signal increase during oxidation of NAP at UTGE. The differential pulse voltammetry (DPV) technique was performed for electrochemical determination of NAP. In the optimum conditions, the limits of detection (LOD) and quantification (LOQ) were determined to be 8.66 10-8 M and 2.88 10-7 M. In addition, the amount of NAP was determined in drug tablets. The recovery studies of NAP from the drug tablet were completed in order to check the accuracy and precision of the applied voltammetric method. Furthermore, the determination of NAP was performed with the high-performance liquid chromatography (HPLC) method. These two methods were compared in terms of accuracy, precision and recovery studies.
Recent pharmacological investigations of bryophytes have proven that the active principles presen... more Recent pharmacological investigations of bryophytes have proven that the active principles present in these plants are quite unique and having potential chemical application and antioxidant capacity. In this study, the volatile components in extracts from Thuidium tamariscinum (Hedw.) Schimp. and Platyhypnidium riparioides (Hedw.) Dixon, Kazdağları (Kalkim-Yenice, Çanakkale, Turkey) were isolated by solid phase micro extraction technique and identified by mass selective detector gas chromatography (GC-MS). Antioxidant capacities of these species were determined by CERAC and CUPRAC methods and phenolic contents by Folin-Ciocalteu method.
In the study, cystic formations obtained from the gills, body cavity and internal organs of some ... more In the study, cystic formations obtained from the gills, body cavity and internal organs of some fish species belonging to the Scombridae family were investigated. Fish materials were obtained from Çanakkale Fish Market. The fish were sampled for six months according to the frequency of their presence in the stalls. In the study, chub mackerel (Scomber japonicus Houttuyn, 1782) and mackerel (Scomber scombrus Linnaeus, 1758) were determined as the main fish samples. The bluefin tuna (Thunnus thynnus Linnaeus, 1758) caught with certain rules between May and July, and samples were also obtained and included in the study based on samples. Samples isolated from gills, body cavities and internal organs were kept in tubes containing absolute ethanol with infected tissue and examined under a stereo and then a light microscope and species identifications were performed using classical methods. Molecular methods were used for identification of nematodes where no clear species could be determi...
A voltammetry method was developed for the direct quantitative determination of clozapine in tabl... more A voltammetry method was developed for the direct quantitative determination of clozapine in tablet dosage forms based on its oxidation behavior. The electrochemical determination of clozapine was easily carried out on glassy carbon electrode (GCE) using a variety of voltammetry techniques. The electrochemical measurements were carried out on GCE in various buffer solutions in the pH range from 3.00 to 12.00 by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The dependence of pH on the anodic peak current and peak potential was investigated. Acetate buffer (pH 5.50) was selected for analytical purposes. The diffusion-controlled nature of the peak was established. A linear calibration curve for DPV analysis was constructed in the clozapine concentration range from 3x10-6 mol L-1 to 1x10-5 mol L-1. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 4.082x10-7 mol L-1 and 1.361x10-6 mol L-1 respectively. The applied voltammetric method was validated.
Homalothecium sericeum (Hedw.) Schimp. is growing in habitats such as walls and roofs. It is supp... more Homalothecium sericeum (Hedw.) Schimp. is growing in habitats such as walls and roofs. It is supported by the studies that moss contains antioxidant, antimicrobial, and antitumoral compounds. It was aimed to determine the immunoreactivity of Cu / Zn SOD enzyme in the kidney, adrenal gland, and ovarian tissues of rats due to the increase in the dose of moss extract. In this study, 1 mL of distilled water were given to control groups (G1), 50 mg/kg (G2), 100 mg/kg (G3), 300 mg/kg (G4), and 500 mg/kg (G5) moss extract were administered by gavage for 30 days another group. At the end of the experiment period, the tissues taken from the rats were subjected to routine histopathological procedures. Cu /Z n SOD primary antibody was applied using immunohistochemical staining methods to detect immunoreactivity. The study was terminated by using the Kruskal-Wallis test, which is one of the nonparametric tests. To determine the differences between the groups by evaluating the stained tissue samples with the image analysis system in the light microscope. A significant difference was found in the dose-related positivity of the kidney, ovarian, and adrenal gland tissues of the group are given moss extract p. It has been determined that H. sericeum species increase Cu/Zn SOD enzyme activity in the kidney, adrenal gland, and ovarian tissues, and its cytotoxic effects show a dose-related increase in the histopathological table.
Bryophytes are the oldest of all land plants and are believed to be the closest remaining link be... more Bryophytes are the oldest of all land plants and are believed to be the closest remaining link between land and aquatic plants. Their soft tissue makes fossil records bleak but the oldest evidence that has so far been found can be dated back to almost 500 million years ago. Spore-like structures of a liverwort were found in Argentinian rock dated to 473-471 million years old. The first evidence of mosses appears much more recently between fossils aged between 299-250 million years old. Due to the poor preservation of Bryophyte species, it is quite possible that the Bryophytes are significantly older. The bryophytes are the second largest group, exceeded only by the Magnoliophyta – the flowering plants (350,000 species). Their nearest algal relatives appear to be members of the Charophyta. Bryophytes are generally considered the first land plants. The role of bryophytes in the ecosystem is significant despite their small size. Aquatic mosses are generally chosen for their aesthetic q...
The fact that there are no real borders between the biosphere, atmosphere, lithosphere, and hydro... more The fact that there are no real borders between the biosphere, atmosphere, lithosphere, and hydrosphere means that environmental pollution monitoring studies should not only include one of the environmental spheres. Thus, integrated environmental pollution assessment studies conducted in the biosphere, lithosphere, and atmosphere promote the “whole system” approach. In this study, the aim was to determine the pollution in the atmosphere, soil, and plants by taking advantage of the high pollution accumulation characteristics of the mosses. Prevailing wind has the potential to distribute pollutants emitted into the air throughout its path. With this regard, soil, mosses, and atmospheric deposition samples were collected in Çanakkale, Turkey, in two seasons. Concentrations of selected elements were measured by Inductively Coupled Plasma-Mass Spectrometry. The enrichment factor of the selected elements in the soil, moss, and deposition samples was calculated. The highest enrichments wer...
Background: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different... more Background: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different drugs that have different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading quercetin (Que) and valproic acid (VPA) into chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of chitosan NPs loaded with a single (Que or VPA) and dual drug (Que and VPA), the characterization of the NPs, the conducting of in vitro antioxidant activity studies, and the analysis of the cytotoxicity and antioxidant activity of the NPs in human neuroblastoma SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H 2 O 2 -induced cell damage were, in order, 96 µg/mL of Que-loaded chitosan NP (77.30%, 48 h), 2 µg/mL of VPA-loaded chitosan NP (70.06%, 24 h), 96 µg/mL of blank chitosan NP (68.31%, 48 h), and 2 µg/mL of Que-and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm for developing drugloaded NPs with a uniform and homogeneous distribution of drugs into NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed. The capability of chitosan NPs developed at the nanometer scale to sustain cell viability in SH-SY5Y cell lines implies the potential of intranasal administration of chitosan NPs for future studies, offering protective effects in central nervous system diseases.
Objective: Diabetes is a disease that occurs due to pancreatic β cells failing to produce enough ... more Objective: Diabetes is a disease that occurs due to pancreatic β cells failing to produce enough insulin or the inability to use the produced insulin effectively in the body. According to the data of the IDF diabetes atlas, it has been reported that there are 537 million diabetic patients aged 20-79 worldwide in 2021 and this number is expected to reach 643 million in 2030 and 783 million in 2045. To control diabetes at such a severe level, scientists are trying to find various treatment methods. One of them is natural treatments. In this study, the affinity values of quercetin, gallic acid, oleanolic acid and ursolic acid, which have antidiabetic properties, to diabetes-related renin, cathepsin-d, T-PA, leptin, MASP-2, FABP4 proteins were investigated. Methods: For molecular docking analysis, unwanted residues and chains were deleted from the proteins with UCSF Chimera 1.15 software and prepared for docking by adding polar hydrogen atoms. Next, quercetin, gallic acid, oleanolic aci...
Objective: According to studies conducted in recent years, pesticides can lead to the development... more Objective: According to studies conducted in recent years, pesticides can lead to the development of cancer, Parkinson's disease, Alzheimer's disease, reproductive system disorders, and birth defects. The aim of this study is to examine the DNA affinity levels of Alpha-cypermethrin, Malathion, Quinclorac, and Roundup (Glyphosate) plant protection products and to discuss them in the light of the literature. Methods: Docking results between ligand and receptor were detected using Hex 8.0.0 software. Preparation of the receptor and ligand for docking was done with UCSF Chimera 1.15 software. Docking visualizations were made with BIOVIA Discovery Studio and PyMol software. While the interaction images of pesticides with DNA were detected with BIOVIA Discovery Studio software, DNA binding images were detected with PyMol software. Results: In our study, the affinity levels of the plant protection products frequently used in the Çanakkale region were determined as Alpha Cypermethri...
In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graph... more In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graphite electrode (UTGE). The cyclic voltammetry (CV) technique was used to determine the optimum conditions and the effect of pH on the electrochemical oxidation of NAP. Acetate buffer (pH 4.50) was selected as the support electrolyte due to obtaining the highest electronic signal increase during oxidation of NAP at UTGE. The differential pulse voltammetry (DPV) technique was performed for electrochemical determination of NAP. In the optimum conditions, the limits of detection (LOD) and quantification (LOQ) were determined to be 8.66 10-8 M and 2.88 10-7 M. In addition, the amount of NAP was determined in drug tablets. The recovery studies of NAP from the drug tablet were completed in order to check the accuracy and precision of the applied voltammetric method. Furthermore, the determination of NAP was performed with the high-performance liquid chromatography (HPLC) method. These two methods were compared in terms of accuracy, precision and recovery studies.
In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graph... more In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graphite electrode (UTGE). The cyclic voltammetry (CV) technique was used to determine the optimum conditions and the effect of pH on the electrochemical oxidation of NAP. Acetate buffer (pH 4.50) was selected as the support electrolyte due to obtaining the highest electronic signal increase during oxidation of NAP at UTGE. The differential pulse voltammetry (DPV) technique was performed for electrochemical determination of NAP. In the optimum conditions, the limits of detection (LOD) and quantification (LOQ) were determined to be 8.66 10-8 M and 2.88 10-7 M. In addition, the amount of NAP was determined in drug tablets. The recovery studies of NAP from the drug tablet were completed in order to check the accuracy and precision of the applied voltammetric method. Furthermore, the determination of NAP was performed with the high-performance liquid chromatography (HPLC) method. These two methods were compared in terms of accuracy, precision and recovery studies.
Recent pharmacological investigations of bryophytes have proven that the active principles presen... more Recent pharmacological investigations of bryophytes have proven that the active principles present in these plants are quite unique and having potential chemical application and antioxidant capacity. In this study, the volatile components in extracts from Thuidium tamariscinum (Hedw.) Schimp. and Platyhypnidium riparioides (Hedw.) Dixon, Kazdağları (Kalkim-Yenice, Çanakkale, Turkey) were isolated by solid phase micro extraction technique and identified by mass selective detector gas chromatography (GC-MS). Antioxidant capacities of these species were determined by CERAC and CUPRAC methods and phenolic contents by Folin-Ciocalteu method.
In the study, cystic formations obtained from the gills, body cavity and internal organs of some ... more In the study, cystic formations obtained from the gills, body cavity and internal organs of some fish species belonging to the Scombridae family were investigated. Fish materials were obtained from Çanakkale Fish Market. The fish were sampled for six months according to the frequency of their presence in the stalls. In the study, chub mackerel (Scomber japonicus Houttuyn, 1782) and mackerel (Scomber scombrus Linnaeus, 1758) were determined as the main fish samples. The bluefin tuna (Thunnus thynnus Linnaeus, 1758) caught with certain rules between May and July, and samples were also obtained and included in the study based on samples. Samples isolated from gills, body cavities and internal organs were kept in tubes containing absolute ethanol with infected tissue and examined under a stereo and then a light microscope and species identifications were performed using classical methods. Molecular methods were used for identification of nematodes where no clear species could be determi...
A voltammetry method was developed for the direct quantitative determination of clozapine in tabl... more A voltammetry method was developed for the direct quantitative determination of clozapine in tablet dosage forms based on its oxidation behavior. The electrochemical determination of clozapine was easily carried out on glassy carbon electrode (GCE) using a variety of voltammetry techniques. The electrochemical measurements were carried out on GCE in various buffer solutions in the pH range from 3.00 to 12.00 by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The dependence of pH on the anodic peak current and peak potential was investigated. Acetate buffer (pH 5.50) was selected for analytical purposes. The diffusion-controlled nature of the peak was established. A linear calibration curve for DPV analysis was constructed in the clozapine concentration range from 3x10-6 mol L-1 to 1x10-5 mol L-1. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 4.082x10-7 mol L-1 and 1.361x10-6 mol L-1 respectively. The applied voltammetric method was validated.
Homalothecium sericeum (Hedw.) Schimp. is growing in habitats such as walls and roofs. It is supp... more Homalothecium sericeum (Hedw.) Schimp. is growing in habitats such as walls and roofs. It is supported by the studies that moss contains antioxidant, antimicrobial, and antitumoral compounds. It was aimed to determine the immunoreactivity of Cu / Zn SOD enzyme in the kidney, adrenal gland, and ovarian tissues of rats due to the increase in the dose of moss extract. In this study, 1 mL of distilled water were given to control groups (G1), 50 mg/kg (G2), 100 mg/kg (G3), 300 mg/kg (G4), and 500 mg/kg (G5) moss extract were administered by gavage for 30 days another group. At the end of the experiment period, the tissues taken from the rats were subjected to routine histopathological procedures. Cu /Z n SOD primary antibody was applied using immunohistochemical staining methods to detect immunoreactivity. The study was terminated by using the Kruskal-Wallis test, which is one of the nonparametric tests. To determine the differences between the groups by evaluating the stained tissue samples with the image analysis system in the light microscope. A significant difference was found in the dose-related positivity of the kidney, ovarian, and adrenal gland tissues of the group are given moss extract p. It has been determined that H. sericeum species increase Cu/Zn SOD enzyme activity in the kidney, adrenal gland, and ovarian tissues, and its cytotoxic effects show a dose-related increase in the histopathological table.
Bryophytes are the oldest of all land plants and are believed to be the closest remaining link be... more Bryophytes are the oldest of all land plants and are believed to be the closest remaining link between land and aquatic plants. Their soft tissue makes fossil records bleak but the oldest evidence that has so far been found can be dated back to almost 500 million years ago. Spore-like structures of a liverwort were found in Argentinian rock dated to 473-471 million years old. The first evidence of mosses appears much more recently between fossils aged between 299-250 million years old. Due to the poor preservation of Bryophyte species, it is quite possible that the Bryophytes are significantly older. The bryophytes are the second largest group, exceeded only by the Magnoliophyta – the flowering plants (350,000 species). Their nearest algal relatives appear to be members of the Charophyta. Bryophytes are generally considered the first land plants. The role of bryophytes in the ecosystem is significant despite their small size. Aquatic mosses are generally chosen for their aesthetic q...
The fact that there are no real borders between the biosphere, atmosphere, lithosphere, and hydro... more The fact that there are no real borders between the biosphere, atmosphere, lithosphere, and hydrosphere means that environmental pollution monitoring studies should not only include one of the environmental spheres. Thus, integrated environmental pollution assessment studies conducted in the biosphere, lithosphere, and atmosphere promote the “whole system” approach. In this study, the aim was to determine the pollution in the atmosphere, soil, and plants by taking advantage of the high pollution accumulation characteristics of the mosses. Prevailing wind has the potential to distribute pollutants emitted into the air throughout its path. With this regard, soil, mosses, and atmospheric deposition samples were collected in Çanakkale, Turkey, in two seasons. Concentrations of selected elements were measured by Inductively Coupled Plasma-Mass Spectrometry. The enrichment factor of the selected elements in the soil, moss, and deposition samples was calculated. The highest enrichments wer...
Uploads
Papers by Ozlem Yayintas