Papers by Henrik R Andersen
Arteriosclerosis, Thrombosis, and Vascular Biology, 1999
Restenosis after balloon dilatation of stenosed coronary arteries is a major clinical problem. Be... more Restenosis after balloon dilatation of stenosed coronary arteries is a major clinical problem. Because T lymphocytes occur in neointima and because cyclosporine inhibits T-lymphocyte proliferation, we tested the hypothesis that cyclosporine would attenuate neointimal proliferation after balloon dilation injury. Rabbits with a balloon-injured aorta, randomized to cyclosporine in the human therapeutic range (nϭ13) or vehicle (nϭ14) were followed up for 5 weeks; as a control for the effect of cyclosporine, half the rabbits received in addition an aorta allograft. Rabbits were clamped at a human plasma cholesterol level of 5 to 7 mmol/L. Cyclosporine did not affect aorta cholesterol accumulation or neointimal proliferation in balloon-injured aortas; however, it attenuated both in transplanted aortas. Likewise, cyclosporine had no effect on endothelial cells at balloon-injured sites, but protected these cells in the transplanted aortas. Infiltration of smooth muscle cells, T lymphocytes, and macrophages were unaffected by cyclosporine in balloon-injured aortas; however, in transplanted aortas, cyclosporine reduced the relative number of T lymphocytes and macrophages but increased the relative number of smooth muscle cells. Finally, in balloon-injured aortas, cyclosporine did not affect expression of vascular adhesion molecule-1, intercellular adhesion molecule-1, or major histocompatibility complex II, but all these cellular activation markers were attenuated by cyclosporine in transplanted aortas. These results suggest that cyclosporine does not attenuate neointimal proliferation after balloon dilatation, and that T lymphocyte-mediated immune responses are not involved in neointimal proliferation after balloon dilatation.
Separation and Purification Technology
The activation of peroxymonosulfate (PMS) to generate radical oxygen species (ROS) for in situ ch... more The activation of peroxymonosulfate (PMS) to generate radical oxygen species (ROS) for in situ chemical oxidation has been shown to be an efficient technology for polishing micropollutants in wastewater effluent. Thus, it is also of great importance to develop environmental-friendly catalysts for PMS activation. The present study employed suspended biogenic manganese oxides (BioMnOx) to activate PMS for the efficient micropollutant removal. In a feasibility test, relatively high removal efficiencies (>80 %) of 14 selected micropollutants were obtained over short reaction times (20 min), in particular for refractory micropollutants such as carbamazepine (98 %), diclofenac (100 %) and metoprolol (81 %). The inhibition effect of coexisting anions to PMS activation was also investigated, which showed that the addition of HCO 3 inhibited this process, while the addition of Clshowed negligible effect. Based on radical quenching experiments, sulfate radical, hydrogen radical and singlet oxygen were all involved in the PMS activation process, while the responsible ROS for micropollutants removal were compound-specific. Furthermore, a novel BioMnOx/PMS system was established by utilising BioMnOx-coated carriers as catalyst, which were obtained from a previously engineered moving bed biofilm reactor (MBBR). This special BioMnOx/PMS system was then applied as an effluent polishing step. Results showed that most micropollutants were efficiently removed through the BioMnOx/PMS system, such as diclofenac (91 %) and sulfamethoxazole (98 %). Lastly, the toxicity of the MBBR effluent was determined by Vibrio fisheri, which showed minimum toxic effect after the polishing treatment.
Journal of Biotechnology, 2014
Site selective chemical modification is a preferred method, employed to prolong the circulation h... more Site selective chemical modification is a preferred method, employed to prolong the circulation half-life of biopharmaceuticals. Cysteines have been used as attachment point for such modification, however, to be susceptible for chemical modification the involved thiol must be in its reduced form. Proteins often contain disulfides, which aid to maintain their tertiary structure and therefore must remain intact. Thus, methods for selectively reducing cysteine residues, introduced through site-directed mutagenesis, are of interest. In this study a macroporous, polymeric monolith was designed for selectively reducing a single cysteine residue inserted in recombinant human growth hormone (hGH). Advantages of such a material are the circumvention of the need to remove the reducing agent after reaction, as well as milder reduction conditions and a concomitant lower risk of reducing the native disulfides. The designed monolith showed very high capacity towards the selective reduction of an unpaired cysteine residue in a recombinant hGH variant. Factors influencing the selectivity and rate of reaction were investigated and it was found that monolith thiol loading, and buffer pH had an effect on the rate of reduction, whereas hGH variant concentration and buffer conductivity influenced both rate of reduction and selectivity. The developed system constitutes the basis for the development of a scalable platform for selective reduction of a capped cysteine residue in hGH.
Applied Catalysis B: Environmental, 2015
Users may download and print one copy of any publication from the public portal for the purpose... more Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
International Journal of Hygiene and Environmental Health, 2017
Users may download and print one copy of any publication from the public portal for the purpose... more Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Users may download and print one copy of any publication from the public portal for the purpose... more Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Water Science and Technology, 2007
The occurrence and fate of parabens in a greywater system was assessed. The potential for removal... more The occurrence and fate of parabens in a greywater system was assessed. The potential for removal of residual paraben concentrations in effluent greywater with chlorine dioxide was also investigated. The influent to the greywater plant was characterised by considerable variation, with concentrations from below the detection limit to 40 μg/L and the five commonly used parabens in consumer products were frequently detected. After the biological treatment only two paraben were detected with concentration from 65–120 ng/L. Chlorine dioxide treatment of the biologically treated effluent with dosages down to 0.75 mg/L resulted in more than 97% reduction of all parabens. Formation of the by-product chloroform was insignificant from the chlorine dioxide treatment.
Science of The Total Environment, 2013
Full bibliographic details must be given when referring to, or quoting from full items including ... more Full bibliographic details must be given when referring to, or quoting from full items including the author's name, the title of the work, publication details where relevant (place, publisher, date), pagination, and for theses or dissertations the awarding institution, the degree type awarded, and the date of the award.
Science of The Total Environment, 2013
Science of The Total Environment, 2013
The widespread occurrence of chlorinated herbicides and their degradation products in the aquatic... more The widespread occurrence of chlorinated herbicides and their degradation products in the aquatic environment raises health and environmental concerns. As a consequence pesticides, and to a lesser degree their degradation products, are monitored by authorities both in surface waters and drinking waters. In this study the formation of degradation products from ultraviolet (UV) treatment of the three chloroacetamide herbicides acetochlor, alachlor and metolachlor and their biological effects were investigated. UV treatment is mainly used for disinfection in water and wastewater treatments. First, the chemical structures of the main UVdegradation products were identified using GC-MS and LC-MS. The main transformation reactions were dechlorination, mono-and multi-hydroxylation and cyclizations. The ecotoxicity of the mixed photoproducts formed by UV-treatment until 90 % of the original pesticide was converted was compared to the toxicity of chloroacetamides using the green alga Pseudokirchneriella subcapitata, the crustacean Daphnia magna and the marine bacteria Vibrio fischeri as test organisms. UV-treatment of alachlor and metolachlor increased the toxicity compared to the parent compounds while an equal toxicity was found for photolysis products of acetochlor. This suggests that toxic photodegradation products are generated from chloroacetamides under UV-treatment. An important perspective of this finding is that the photolysis products are at least as toxic as the parent compounds.
International Journal of Environmental Science and Technology, 2013
Environmental Technology, 2012
Chemical Engineering Journal, 2012
Biologically treated wastewater spiked with a mixture of 56 active pharmaceutical ingredients (AP... more Biologically treated wastewater spiked with a mixture of 56 active pharmaceutical ingredients (APIs) was treated with 0-20 mg/L chlorine dioxide (ClO 2) solution in laboratory-scale experiments. Wastewater effluents were collected from two wastewater treatment plants in Sweden, one with extended nitrogen removal (low COD) and one without (high COD). About one third of the tested APIs resisted degradation even at the highest ClO 2 dose (20 mg/L), while others were reduced by more than 90% at the lowest ClO 2 level (0.5 mg/L). In the low COD effluent, more than half of the APIs were oxidized at 5 mg/L ClO 2 , while in high COD effluent a significant increase in API oxidation was observed after treatment with 8 mg/L ClO 2. This study illustrates the successful degradation of several APIs during treatment of wastewater effluents with chlorine dioxide.
We reconfigured staged MBBR systems with the aim to increase biodegradation of pharmaceuticals an... more We reconfigured staged MBBR systems with the aim to increase biodegradation of pharmaceuticals and achieve an effluent quality that required the lowest ozone dose to remove pharmaceuticals. The MBBR contact tanks intended for nitrification in traditional systems, were doubled in size to provide more biofilm surface area per treated volume of the carbon starved biofilm, characterizing the nitrifying stages of conventional municipal MBBR systems. The systems were operated on hospital wastewater and removal of hardly degradable pharmaceuticals were faster in the middle compartment of the treatment train. The very recalcitrant pharmaceuticals, Carbamazepine, Dichlofenac and Clofibric acid appeared to be very slowly degradable by the MBBR.
Process Safety and Environmental Protection, 2022
Journal of Hazardous Materials, 2021
Conventional wastewater treatment plants (WWTPs) cannot remove organic micropollutants efficientl... more Conventional wastewater treatment plants (WWTPs) cannot remove organic micropollutants efficiently, and thus various polishing processes are increasingly being studied. One such potential process is utilising biogenic manganese oxides (BioMnOx). The present study operated two moving bed biofilm reactors (MBBRs) with synthetic sewage as feed, one reactor feed was spiked with Mn(II) which allowed the continuous formation of BioMnOx by Mn-oxidising bacteria in the suspended biofilms (i.e. BioMn-MBBR). Spiking experiments with 14 micropollutants were conducted to investigate if BioMnOx combined with MBBR could be utilised to polish micropollutants in wastewater treatment. Results show enhanced removal by BioMn-MBBR over control MBBR (without BioMnOx) for specific micropollutants, such as diclofenac (36% vs. 5%) and sulfamethoxazole (80% vs. 24%). However, diclofenac removal was significantly inhibited when municipal wastewater was fed, and a further batch experiment demonstrates the reduced removal of diclofenac could be due to (unusual) higher pH in municipal wastewater compared to synthetic sewage. A shift in bacterial community was also observe in BioMn-MBBR over long-term operation. Overall, BioMn-MBBR in this study shows great potential for practical application in removing a larger range of micropollutants, which could be applied as an efficient polishing step for typical municipal wastewater.
Linnaeus Eco-Tech, 2007
The presence of steroid hormones and endocrine disrupting compounds (EDC) in theenvironment has b... more The presence of steroid hormones and endocrine disrupting compounds (EDC) in theenvironment has been connected with the drop in semen quality in men and the number ofhem1aphrodite fish observed downstream wastewater treatment plants. EDC originating fromdown-the-drain chemicals can be reduced by mitigation options but the naturally occurringhormones must be removed though end-of-pipe treatment. In this study, coagulation andflocculation as well as these two techniques combined with sorption were applied to removeestrone, I 7P-estradiol and the synthetic hormone I7a-ethynylestradiol as well as thepreservatives methyl paraben, ethyl paraben, propyl paraben, butyl paraben and isobutylparaben from primary and secondary treated municipal wastewater. It was found thatcoagulation with both iron and aluminium together with an anionic flocculant successfullyremoved organic matter and dissolved phosphorous but not the hormones and only up to 30%of the parabens. This was seen both in the chemi...
Journal of Environmental Chemical Engineering, 2018
Users may download and print one copy of any publication from the public portal for the purpose... more Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
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Papers by Henrik R Andersen