vega lee

Dalian University of Technology, School of Software, Graduate Student

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Papers by vega lee

Quantification of bcl-2/JH fusion sequences and a control gene by multiplex real-time PCR coupled with automated amplicon sizing by capillary …

Journal of Molecular …, Jan 1, 2002

Follicular lymphoma is characterized by the presence of the t(14;18)(q32;q21) chromosomal translo... more Follicular lymphoma is characterized by the presence of the t(14;18)(q32;q21) chromosomal translocation which juxtaposes the bcl-2 gene at 18q21 with the immunoglobulin heavy chain locus at 14q32. Quantification of t(14;18) carrying cells in FL patients can be achieved by real-time PCR, a highly sensitive technique for evaluating treatment efficacy and minimal residual disease. Despite the many advantages of realtime technology for this purpose, one disadvantage is that current real-time t(14;18) PCR assays amplify a control gene as a normalizer in a separate reaction. Since each PCR reaction has its own kinetics, separate PCR assays for target and control sequences can potentially result in inaccurate quantification of t(14; 18)-positive cells. In addition, the real-time t(14;18) PCR assays do not determine the size of the amplified fusion sequence, which is helpful for excluding contamination and is commonly used to demonstrate clonal identity between pre-and post-treatment specimens from a patient. To address these limitations, we designed a multiplex real-time PCR protocol that allows amplification of control and target genes in the same reaction and precise size determination of bcl-2/JH fusion sequences by capillary electrophoresis. This multiplex PCR assay is equally sensitive to previous assays, allows more accurate quantification of bcl-2/JH fusion sequences, and is more convenient.

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Reheating the post inflationary universe

Arxiv preprint hep-ph/ …, Jan 1, 1995

We consider the non-equilibrium evolution of the inflaton field coupled to both lighter scalars a... more We consider the non-equilibrium evolution of the inflaton field coupled to both lighter scalars and fermions. The dissipational dynamics of this field is studied and found to be quite different than that believed in inflationary models. In particular, the damping time scale for the expectation value of the zero momentum mode of the inflaton can be much shorter than that given by the single particle decay rate when the inflaton amplitudes are large, as in chaotic inflation scenarios. We find that the reheating temperature may depart considerably from the usual estimates. 98.80.Cq, 11.15.Tk

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Proton line narrowing in solid-state nuclear magnetic resonance: New insights from windowed phase-modulated Lee-Goldburg sequence

The Journal of chemical physics, Jan 1, 2006

We present here a bimodal Floquet analysis of the windowed phase-modulated Lee-Goldburg (wPMLG) s... more We present here a bimodal Floquet analysis of the windowed phase-modulated Lee-Goldburg (wPMLG) sequence for homonuclear dipolar decoupling. One of the main criteria for an efficient homonuclear dipolar decoupling scheme is an effective z-rotation condition. This is brought about by the presence of radio-frequency imperfections in the pulse sequence together with a systematic manipulation of the wPMLG pulses. Additional improvement in the (1)H spectral resolution was obtained by a proper understanding of the off-resonance dependence of the wPMLG irradiation scheme based on bimodal Floquet theory. Numerical investigations further corroborate both theoretical and experimental findings. Theoretical analysis points to accidental degeneracies between the cycle time of the wPMLG sequence and the rotor period leading to the experimentally observed off-resonance dependence of the resolution. Two-dimensional (1)H-(1)H homonuclear single-quantum correlation spectra of model amino acids are also presented, highlighting the improved spectral resolution of wPMLG sequences.

Mid-infrared imaging of candidate Vega-like systems

The Astronomical …, Jan 1, 2001

We have conducted deep mid-infrared imaging of a relatively nearby sample of candidate Vega-like ... more We have conducted deep mid-infrared imaging of a relatively nearby sample of candidate Vega-like stars using the OSCIR instrument on the CTIO 4-meter and Keck II 10-meter telescopes. Our discovery of a spatially-resolved disk around HR 4796A has already been reported ). Here we present imaging observations of the other members of the sample, including the discovery that only the primary in the HD 35187 binary system appears to harbor a substantial circumstellar disk and the possible detection of extended disk emission around 49 Ceti.

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The NO TRAIL to YES TRAIL in cancer therapy (Review)

International …, Jan 1, 2007

Treatment of cancer patients with conventional therapies (chemotherapy, hormonal therapy, immunot... more Treatment of cancer patients with conventional therapies (chemotherapy, hormonal therapy, immunotherapy and radiation) respond initially well and experience prolonged tumor-free survival. However, in many patients tumor recurrences and relapses occur and such tumors exhibit the resistant phenotype i.e. cross-resistance to various cytotoxic and apoptotic agents. Therefore, new therapeutic strategies are currently being explored and are based on a better understanding of the underlying biochemical and molecular mechanisms of tumor cell resistance. Hence, novel sensitizing agents that can modify the tumor dysregulated apoptotic gene products can reverse resistance when used in combination with subtoxic doses of cytotoxic reagents. Targeted antitumor therapies are the current choice in the treatment of resistant tumors. One such targeted therapy is the application of TRAIL or TRAIL agonist monoclonal antibodies (mAbs) (anti-DR4 and anti-DR5) because, unlike Fas ligand and TNF-α, they are not cytotoxic to normal tissues. TRAIL as monotherapy will only be effective against TRAIL sensitive tumors, however, most tumors are resistant to TRAIL and their sensitization can restore their sensitivity to TRAIL apoptosis. We present, herein, one potential novel sensitizing agent, namely, nitric oxide (NO) that has been shown to sensitize TRAIL-resistant tumor cells to TRAIL apoptosis via its inhibitory effect on the transcription factors NF-κB and Yin Yang 1 (YY1), concomitantly with upregulation of DR5. We propose the therapeutic application of NO donors as sensitizing agents used in combination with TRAIL/DR4 or DR5 mAbs in the treatment of TRAIL-resistant tumors.

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The effects of growth hormone-releasing peptide-2 (GHRP-2) on the release of growth hormone and growth performance in swine

Domestic Animal …, Jan 1, 2000

The effects of GHRP-2 (also named KP102), a new growth hormone-releasing peptide, on the release ... more The effects of GHRP-2 (also named KP102), a new growth hormone-releasing peptide, on the release of growth hormone (GH) and growth performance were examined in swine. The single intravenous (i.v.) injection of GHRP-2 at doses of 2, 10, 30 and 100 g/kg body weight (BW) to cross-bred castrated male swine stimulated GH release in a dose-dependent manner, with a return to the baseline by 120 min. The peak GH concentrations and GH areas under the response curves (GH AUCs) for 180 min after the injections of GHRP-2 were higher (P Ͻ 0.05) than those after the injection of saline. The GH responses to repeated i.v. injections of GHRP-2 (30 g/kg BW) at 2-h intervals for 6 h were decreased after each injection. The chronic subcutaneous (s.c.) administration of GHRP-2 (30 g/kg BW) once daily for 30 days consistently stimulated GH release. The GH AUCs for 300 min after the injections on d 1, 10 and 30 of treatment in GHRP-2-treated swine were higher than those in saline-treated swine. However, chronic administration of GHRP-2 caused a partial attenuation of GH response between d 1 and 10 of treatment. The chronic s.c. administration of GHRP-2 also increased average daily gain for the entire treatment period by 22.35% (P Ͻ 0.05) and feed efficiency (feed/gain) by 20.64% (P Ͻ 0.01) over the saline control values, but did not significantly affect daily feed intake. These results indicate that GHRP-2 stimulates GH release and enhancing growth performance in swine.

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A broad-banded z-rotation windowed phase-modulated Lee-Goldburg pulse sequence for 1H spectroscopy in solid-state NMR

Chemical Physics Letters, Jan 1, 2007

High-resolution 1 H spectroscopy in solid-state NMR, rendered difficult due to the strong 1 H-1 H... more High-resolution 1 H spectroscopy in solid-state NMR, rendered difficult due to the strong 1 H-1 H homonuclear dipolar coupling, has been made possible under magic-angle spinning with homonuclear dipolar decoupling schemes, such as windowed phase-modulated Lee-Goldburg. Here, we outline the theory and implementation of a modification of this scheme with which an effective z-rotation for the magnetisation is obtained over a wide range of spectral window. Experimental results are presented for samples, such as glycine, histidine, and tryosine.

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Long-term results of angioplasty vs stenting in cardiac transplant recipients with allograft vasculopathy

The Journal of heart and …, Jan 1, 2005

Background: Allograft vasculopathy remains a major limiting factor in long-term graft survival. T... more Background: Allograft vasculopathy remains a major limiting factor in long-term graft survival. The absence of symptoms and diffuse nature of the disease make clinical detection and therapy more difficult. Limited data exist on the long-term outcome of percutaneous interventions in this group of patients.

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Quantification of bcl-2/JH fusion sequences and a control gene by multiplex real-time PCR coupled with automated amplicon sizing by capillary …

Journal of Molecular …, Jan 1, 2002

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Reheating the post inflationary universe

Arxiv preprint hep-ph/ …, Jan 1, 1995

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Proton line narrowing in solid-state nuclear magnetic resonance: New insights from windowed phase-modulated Lee-Goldburg sequence

The Journal of chemical physics, Jan 1, 2006