Posttranslational modifications of NF-κB, including phosphorylation, acetylation, and methylation... more Posttranslational modifications of NF-κB, including phosphorylation, acetylation, and methylation, have emerged as important regulatory mechanisms to control the transcriptional outcomes of this important transcription factor. These modifications work independently, sequentially or in combination to modulate the diverse biological functions of NF-κB in cancer and inflammatory response. Here, we describe some experimental methods to detect the in vitro and in vivo phosphorylation and acetylation of NF-κB, specifically focusing on the RelA subunit of NF-κB. These methods include labeling the phospho-or acetyl-groups with radioisotopes in vitro and immunoblotting with site-specific anti-phospho-serine or acetyl-lysine antibodies in culture cells and tissue samples.
With the advent of technology, input output resources and high speed networks the manual examinat... more With the advent of technology, input output resources and high speed networks the manual examination systems of Education field is now replaced by the online examination systems or cloud based online examination systems. Till date, the common problem in manual examination system of assigning tests and evaluating their scores was time consuming and monotonous. This process raises several disadvantages like discrepancy in calculating the scores and displaying the grades of students. These problems of manual examination system can be overcome by cloud based Online Examination System. The literature survey in this domain states that such kind of systems are designed using the technologies like –M-learning algorithm and its Android application, Automatic correction and optimization of MCQ test answer sheets and the usage of smart adaptive algorithms etc. This paper summarizes various tools and algorithms that are used to design and implement online examination system.
Video analytics is used to analyse video streams in near real-time and for identifying characteri... more Video analytics is used to analyse video streams in near real-time and for identifying characteristics, actions or patterns of specific behaviour via analysis of monitored environments. It may include technical functions such as classification and detection of objects, face recognition, vehicle recognition and motion detection using trained models. The technology used to achieve video analytics may include sensors, cameras and connectivity techniques. Video analytics comes with many advantages to businesses, especially in security surveillance systems. This paper presents an overview of a Video Analytics tool OpenVINO developed by Intel®. OpenVINO combines concepts such as Computer Vision and Deep Learning to analyse the video content and to extract meaningful information.
International Journal of Applied Dental Sciences, 2020
Archwires are a very important and integral part of treatment with fixed orthodontic appliances. ... more Archwires are a very important and integral part of treatment with fixed orthodontic appliances. With the development in the field of orthodontics, many newer arch wires have come up that provides a clinician variety of options to choose from. These newer wires provides a lot of advantages over conventional wires in terms of efficiency, total treatment time, and finishing. Moreover, the demand for esthetic treatment options in orthodontics led to the development of esthetic archwires. These new archwires try to combine both esthetics as well as good clinic performance. The knowledge of these archwires is absolutely imperative to keep up with the newer trends.
Abstract Individuals utilize diets as a way to lose weight. Since not all diets work the same nor... more Abstract Individuals utilize diets as a way to lose weight. Since not all diets work the same nor do all people have the same metabolism some approaches may be more deleterious than beneficial. For dietitians the dieters alike, an understanding of the options that exist in addition to what they entail is advantageous for all. However, not all diets are feasible for all individuals, either for financial reasons or due to co-morbidities. Diets can generally be categorized into five groups: carbohydrate-limiting, Caveman-Like, miscellaneous, commercialized, and lifestyle modification. This review explores how diets grouped into the aforementioned categories work, some clinically supported evidence indicating pros and cons, and finally a discussion on how the Mediterranean and DASH Diets seem to show the most promise in terms of weight loss.
Arsenic is a major contaminating heavy metal due to its frequent occurrence and toxicity. Out of ... more Arsenic is a major contaminating heavy metal due to its frequent occurrence and toxicity. Out of different remediation approaches, bioremediation using bacteria has been extensively studied. In this study, 29 bacterial isolates were screened for their arsenic tolerance capacity. One of the isolate, MUM2 showed maximum arsenic tolerance (10mM). Antioxidant enzymes were assayed in this isolate under arsenic stress. Although, the CAT activity was found to increase significantly, the SOD activity showed a significant decrease under 9mM arsenic stress. The results obtained suggest a possible role of CAT in combating arsenic stress. Further studies at genetic level would help in developing highly tolerant strains for remediation of arsenic. V
International Biodeterioration & Biodegradation, 2013
ABSTRACT Phenol is a common pollutant which is found in wastewater of many industries and removal... more ABSTRACT Phenol is a common pollutant which is found in wastewater of many industries and removal of phenol from the industrial effluents is a major challenge. Recently, the use of hairy roots has been probed for the removal of phenol. In the present study, phenol at various concentrations (100–500 mg L−1) was treated with hairy roots of Helianthus annuus (sunflower hairy roots, SHRs). SHRs removed 100 mg L−1 of phenol after 144 h of incubation. The effect of polyethylene glycol (PEG), l-proline and d-glucose on the rate of phenol removal was also studied. l-proline enhanced the removal efficiency of SHRs resulting in the removal of 100 mg L−1 of phenol after 24 h while PEG did not show any effect on removal. Peroxidase activity was induced after 24 h of phenol addition. Phenol metabolism to generate catechol as a major metabolite was confirmed using HPLC and GC–MS analyses. The detection of small amounts of cis-cis muconic acid and fumaric acid in the reaction medium suggests that these metabolites are produced from the ring cleavage of catechol. The phytotoxicity and cytotoxicity results suggest the non-toxic nature of the resulting phenol metabolites.
RUNX3 is a transcription factor with regulatory roles in cell proliferation and development. Whil... more RUNX3 is a transcription factor with regulatory roles in cell proliferation and development. While largely characterized as a tumor suppressor, RUNX3 can also be oncogenic in certain cancers. Many factors account for the tumor suppressor function of RUNX3, which is reflected by its ability to suppress cancer cell proliferation after expression-restoration, and its inactivation in cancer cells. Ubiquitination and proteasomal degradation represent a major mechanism for the inactivation of RUNX3 and the suppression of cancer cell proliferation. On the one hand, RUNX3 has been shown to facilitate the ubiquitination and proteasomal degradation of oncogenic proteins. On the other hand, RUNX3 can be inactivated through the ubiquitin–proteasome system. This review encapsulates two facets of RUNX3 in cancer: how RUNX3 suppresses cell proliferation by facilitating the ubiquitination and proteasomal degradation of oncogenic proteins, and how RUNX3 is degraded itself through interacting RNA-, p...
Posttranslational modifications of NF-κB, including phosphorylation, acetylation, and methylation... more Posttranslational modifications of NF-κB, including phosphorylation, acetylation, and methylation, have emerged as important regulatory mechanisms to control the transcriptional outcomes of this important transcription factor. These modifications work independently, sequentially or in combination to modulate the diverse biological functions of NF-κB in cancer and inflammatory response. Here, we describe some experimental methods to detect the in vitro and in vivo phosphorylation and acetylation of NF-κB, specifically focusing on the RelA subunit of NF-κB. These methods include labeling the phospho-or acetyl-groups with radioisotopes in vitro and immunoblotting with site-specific anti-phospho-serine or acetyl-lysine antibodies in culture cells and tissue samples.
With the advent of technology, input output resources and high speed networks the manual examinat... more With the advent of technology, input output resources and high speed networks the manual examination systems of Education field is now replaced by the online examination systems or cloud based online examination systems. Till date, the common problem in manual examination system of assigning tests and evaluating their scores was time consuming and monotonous. This process raises several disadvantages like discrepancy in calculating the scores and displaying the grades of students. These problems of manual examination system can be overcome by cloud based Online Examination System. The literature survey in this domain states that such kind of systems are designed using the technologies like –M-learning algorithm and its Android application, Automatic correction and optimization of MCQ test answer sheets and the usage of smart adaptive algorithms etc. This paper summarizes various tools and algorithms that are used to design and implement online examination system.
Video analytics is used to analyse video streams in near real-time and for identifying characteri... more Video analytics is used to analyse video streams in near real-time and for identifying characteristics, actions or patterns of specific behaviour via analysis of monitored environments. It may include technical functions such as classification and detection of objects, face recognition, vehicle recognition and motion detection using trained models. The technology used to achieve video analytics may include sensors, cameras and connectivity techniques. Video analytics comes with many advantages to businesses, especially in security surveillance systems. This paper presents an overview of a Video Analytics tool OpenVINO developed by Intel®. OpenVINO combines concepts such as Computer Vision and Deep Learning to analyse the video content and to extract meaningful information.
International Journal of Applied Dental Sciences, 2020
Archwires are a very important and integral part of treatment with fixed orthodontic appliances. ... more Archwires are a very important and integral part of treatment with fixed orthodontic appliances. With the development in the field of orthodontics, many newer arch wires have come up that provides a clinician variety of options to choose from. These newer wires provides a lot of advantages over conventional wires in terms of efficiency, total treatment time, and finishing. Moreover, the demand for esthetic treatment options in orthodontics led to the development of esthetic archwires. These new archwires try to combine both esthetics as well as good clinic performance. The knowledge of these archwires is absolutely imperative to keep up with the newer trends.
Abstract Individuals utilize diets as a way to lose weight. Since not all diets work the same nor... more Abstract Individuals utilize diets as a way to lose weight. Since not all diets work the same nor do all people have the same metabolism some approaches may be more deleterious than beneficial. For dietitians the dieters alike, an understanding of the options that exist in addition to what they entail is advantageous for all. However, not all diets are feasible for all individuals, either for financial reasons or due to co-morbidities. Diets can generally be categorized into five groups: carbohydrate-limiting, Caveman-Like, miscellaneous, commercialized, and lifestyle modification. This review explores how diets grouped into the aforementioned categories work, some clinically supported evidence indicating pros and cons, and finally a discussion on how the Mediterranean and DASH Diets seem to show the most promise in terms of weight loss.
Arsenic is a major contaminating heavy metal due to its frequent occurrence and toxicity. Out of ... more Arsenic is a major contaminating heavy metal due to its frequent occurrence and toxicity. Out of different remediation approaches, bioremediation using bacteria has been extensively studied. In this study, 29 bacterial isolates were screened for their arsenic tolerance capacity. One of the isolate, MUM2 showed maximum arsenic tolerance (10mM). Antioxidant enzymes were assayed in this isolate under arsenic stress. Although, the CAT activity was found to increase significantly, the SOD activity showed a significant decrease under 9mM arsenic stress. The results obtained suggest a possible role of CAT in combating arsenic stress. Further studies at genetic level would help in developing highly tolerant strains for remediation of arsenic. V
International Biodeterioration & Biodegradation, 2013
ABSTRACT Phenol is a common pollutant which is found in wastewater of many industries and removal... more ABSTRACT Phenol is a common pollutant which is found in wastewater of many industries and removal of phenol from the industrial effluents is a major challenge. Recently, the use of hairy roots has been probed for the removal of phenol. In the present study, phenol at various concentrations (100–500 mg L−1) was treated with hairy roots of Helianthus annuus (sunflower hairy roots, SHRs). SHRs removed 100 mg L−1 of phenol after 144 h of incubation. The effect of polyethylene glycol (PEG), l-proline and d-glucose on the rate of phenol removal was also studied. l-proline enhanced the removal efficiency of SHRs resulting in the removal of 100 mg L−1 of phenol after 24 h while PEG did not show any effect on removal. Peroxidase activity was induced after 24 h of phenol addition. Phenol metabolism to generate catechol as a major metabolite was confirmed using HPLC and GC–MS analyses. The detection of small amounts of cis-cis muconic acid and fumaric acid in the reaction medium suggests that these metabolites are produced from the ring cleavage of catechol. The phytotoxicity and cytotoxicity results suggest the non-toxic nature of the resulting phenol metabolites.
RUNX3 is a transcription factor with regulatory roles in cell proliferation and development. Whil... more RUNX3 is a transcription factor with regulatory roles in cell proliferation and development. While largely characterized as a tumor suppressor, RUNX3 can also be oncogenic in certain cancers. Many factors account for the tumor suppressor function of RUNX3, which is reflected by its ability to suppress cancer cell proliferation after expression-restoration, and its inactivation in cancer cells. Ubiquitination and proteasomal degradation represent a major mechanism for the inactivation of RUNX3 and the suppression of cancer cell proliferation. On the one hand, RUNX3 has been shown to facilitate the ubiquitination and proteasomal degradation of oncogenic proteins. On the other hand, RUNX3 can be inactivated through the ubiquitin–proteasome system. This review encapsulates two facets of RUNX3 in cancer: how RUNX3 suppresses cell proliferation by facilitating the ubiquitination and proteasomal degradation of oncogenic proteins, and how RUNX3 is degraded itself through interacting RNA-, p...
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