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Imre Semsei

University of Debrecen, Faculty of Health, Department of Gerontology, Faculty Member

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Address: Faculty of Health, University of Debrecen
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Papers by Imre Semsei

The effects of idebenone on DNA and RNA contents as well as synthesis rates of total and poly(A)+ RNA in brain of normal, old C57BL/6J mice and in experimental partial cerebral ischemia of rats

Archives of Gerontology and Geriatrics, Nov 1, 1990

Summa~ Effects of idebenone on RNA and DNA contents as well as on synthesis rates of total and po... more Summa~ Effects of idebenone on RNA and DNA contents as well as on synthesis rates of total and poly(A) + RNA in the brain were measured in two animal models: (1) Normal young and old, male C57BL/6J mice (6 and 32 months). Idebenone suspended in 5% gum arabic was applied in 50 mg/kg/day dose to old mice for 1 month through a gastric tube. (2) Adult female CFY rats (14 18 months) in which experimental partial cerebral ischemia was induced by bilateral common carotid artery occlusion. Idebenone was administered intraperitoneally in two doses (10 mg/kg and 100 mg/kg body weight) 30 min before the interruption of carotid blood flow. DNA content remained invariate during aging in the brain; idebenone treatment did not exert any influence on this parameter. RNA content as well as total and poly(A) + RNA synthesis rates, which were measured by incorporation of tritiated uridine into RNA, decreased significantly with age in brain. Idebenone treatment did not cause any essential change of the metabolism of RNA under the given conditions. The RNA and DNA contents of brain were influenced neither by experimental partial cerebral ischemia nor by treatment with idebenone during the ischemia. Partial cerebral ischemia decreased the rate of total and poly(A) + RNA synthesis in the brain about 15-45% depending on the methods and basis of expression. This decline could totally be prevented by intraperitoneal application of 10 mg/kg idebenone 30 rain before the onset of the partial ischemia. The dose of 100 mg/kg idebenone also elevated the rate of RNA synthesis; however, this increase remained statistically insignificant.

Tissue and age specific expression of the myc proto-oncogene family throughout the life span of the C57BL/6J mouse strain

PubMed, Apr 1, 1989

The expression of the proto-oncogene myc family (c, L and N) in terms of steady-state mRNA levels... more The expression of the proto-oncogene myc family (c, L and N) in terms of steady-state mRNA levels was determined in seven different normal non-cancerous tissues throughout the life span (seven different ages) of the C57BL/6J male mouse strain. C-myc oncogene expression was highest in prenatal and newborn ages and then decreased to its lowest levels at about 6 months of age. With further increase of age, a progressive pattern of increase in expression of c-myc was found in brain, liver, skin, and small intestine. However, for kidney, spleen and heart, little or no significant change was evident. Significant differential expression of c-myc was found in most tissues in animals of the same age, with highest expression consistently being found in spleen and liver at all ages. For the N-myc and L-myc oncogenes, expression was also highest in prenatal and newborn tissue as compared to the 6-month young adult, but little or no further change was found at older ages. However, substantial tissue-dependent differences in expression were also found, and no expression at all was detected at any age for N-myc in liver and for L-myc in heart, small intestine and liver. Taken together, these results indicate that the expression of the proto-oncogenes c-, L- and N-myc is dependent not only on tissue and embryonic development, as previously shown by other workers, but also on age past the young adult stage of life span. The age-dependent increase in expression of c-myc oncogene found in normal-appearing non-cancerous tissues is of particular interest as possibly reflecting tissue alterations related to both the aging process and the age-dependent increase in cancer incidence.

One gene, two transcripts: isolation of an alternative transcript encoding for the autoantigen La/SS-B from a cDNA library of a patient with primary Sjögrens' syndrome

Journal of Experimental Medicine, Dec 1, 1994

A cDNA library was prepared from peripheral blood lymphocytes of an autoimmune patient with prima... more A cDNA library was prepared from peripheral blood lymphocytes of an autoimmune patient with primary Sj6grens' syndrome. The cDNA library was screened with the patients own autoimmune serum being monospecitic for the nuclear autoantigen La/SS-B. Thereby an alternative type of La mRNA was identified that differed from the known La mRNA due to an exchange of the exon 1. Sequencing of the genomic region between the exons 1 and 2 showed that the alternative 5'-end is a part of the intron. In addition, the presence of an alternative promoter site, which exists within the intron downstream of the exon 1, became evident. In consequence, the alternative La mRNA is the result of a promoter switching combined with an alternative splicing mechanism. In the intron, further transcription factor binding sites, including a NF-xB element, were identified leading to the suggestion that the expression of the gene encoding for the nuclear autoantigen La/SS-B alters in dependence on disease conditions.

In vivo stimulation of nerve cells by phytohemagglutinin. II. Alterations in the rate of total and mRNA synthesis in the brain cortex of old rats

Archives of Gerontology and Geriatrics, Dec 1, 1983

Summaw Bacto-phytohemagglutinin-P (PHA-P) was administered in form of a single intralumbar inject... more Summaw Bacto-phytohemagglutinin-P (PHA-P) was administered in form of a single intralumbar injection of 2 mg/100 g body weight dose to 24-to 28-mth-old female CFY rats. The accuracy of the injection technique was checked by adding 2% lidocaine to the injection mixture, which resulted in a transient and symmetric paralysis of the posterior limbs when reaching the cerebrospinal fluid. The total RNA content of the liver and brain cortex were measured, and phenolic extraction of RNA was performed on the brain cortex. Poly(A) + RNA (mRNA) was separated from the total RNA of the brain cortex by oligo(dT)-cellulose chromatography. Pulse labeling with tritiated uridine was performed 45 min before killing the animals and the incorporation of the radiolabel was measured in the respective RNA classes and corrected for the nucleotide pool size. The rates of total and mRNA synthesis are expressed in percentages of the young untreated rats and compared to old untreated animals. The effects of PHA-P was studied at 4, 10, 20 and 44 h after its injection. A considerable increase of the total RNA content of the brain cortex was measured during the first 10 h of the experiment followed by a slow decrease. However, the RNA content of the brain cortex was still significantly higher at the end of experiment compared to untreated old rats. The rate of total RNA synthesis increased significantly during the first 10 h and remained constantly high until 44 h. The rate of mRNA synthesis increased to a higher extent than that of the total RNA, and also remained high until 44 h. membrane hypothesis of aging; phytohemagglutinin; aging of brain cells; rate of RNA synthesis; rate of mRNA synthesis

Centrophenoxine increases the rates of total and mRNA synthesis in the brain cortex of old rats: An explanation of its action in terms of the membrane hypothesis of aging

Experimental Gerontology, 1984

The rates of total and polyA + RNA (mRNA) synthesis were measured by radioisotope technique in th... more The rates of total and polyA + RNA (mRNA) synthesis were measured by radioisotope technique in the brain cortex of female CFY rats. There was practically no significant difference between the young (1.5 months) and adult (13 months) rats; however, the old group (26 months) displayed a considerable decrease of the rates of synthesis of both classes of RNA studied. Centrophenoxine treatment (100 mg per kg body weight per day, for 2 months) reversed this tendency, and increased significantly the synthesis rates of old rats almost to the adult level. The results are interpreted in terms of the membrane hypothesis of aging, attributing a free-radical scavenger function of the dimethylamino-ethanol incorporated into the nerve cell membrane from the centrophenoxine.

Effects of ionic strength on the activity of superoxide dismutase in vitro

Archives of Gerontology and Geriatrics, Dec 1, 1984

Changes in superoxide dismutase (SOD) activity were studied in vitro at increasing NaC1 or KC1 co... more Changes in superoxide dismutase (SOD) activity were studied in vitro at increasing NaC1 or KC1 concentrations. SOD activity was measured using two different systems of superoxide radical generation: pyrogallol autoxidation, and xanthine-xanthine oxidase reaction. Pyrogallol autoxidation was directly measured by spectrophotometry, whereas in the second case cytochrome c reduction was followed at 550 nm. The inhibition of SOD on those parameters was taken as measure of SOD activity. Increasing concentrations of NaCl and KC1 significantly increased the rate of pyrogallol autoxidation. The inhibitory effect of SOD significantly decreased under the influence of these salts and followed an exponential curve. The two salts studied resulted in essentially identical changes in SOD activity. Increasing concentrations of NaC1 and KCI decreased the rate of cytochrome c reduction in the xanthine-xanthine oxidase system. When correcting the results for these primary effects, SOD activity also displayed in this system an exponential decay with increasing salt concentrations. The results are interpreted in terms of the known charge distribution pattern on the surface of the SOD molecule, and of the age-dependent increase of the intracellular potassium and sodium concentrations in the postmitotic cells. superoxide dismutase; ionic strength dependence of SOD activity; intracellular ionic composition during aging; pyrogallol autoxidation for measuring SOD activity; xanthine-xanthine oxidase reaction; cytochrome c reduction

[The presence of t(14;18) chromosome translocation in various types of diseases]

PubMed, Dec 28, 1997

Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic f... more Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic follicular lymphomas. They are causative factors of lymphomas but the translocation is present in different other types of diseases although the translocation does not belong to the features of these illnesses. Our present work shows the appearance of t(14;18) translocation in lymphocytes of two patients of Sjögren's syndrome, one that of Whipple disease as well as one of healthy donors' lymphocytes using polymerase chain reaction technique presented in one of our previous publication. The translocation occurred in the mbr of bcl-2 gene in all cases showed and the bcl-2 gene was coupled with the immunoglobulin heavy chain gene. These results are definitively positive concerning the fact of translocation as it has been proved by sequencing of the amplification products showed in our earlier and present paper. Because relatively high percentages of Sjögren's syndrome patients develop later on lymphoma, the early detection of the translocation could result in a more successful diagnosis as well as treatment of the disease. The question arises, however, what role the translocation plays in illnesses such as the Whipple disease or what kind of consequences can be drawn from the appearance of the t(14;18) translocation in lymphocytes of healthy donors.

In vivo studies on the age-dependent decrease of the rates of total and mRNA synthesis in the brain cortex of rats

Archives of Gerontology and Geriatrics, 1982

The membrane hypothesis of aging (Zs.-Nagy, I., 1978, J. Theor. Biol. 75, 189-195) attributes the... more The membrane hypothesis of aging (Zs.-Nagy, I., 1978, J. Theor. Biol. 75, 189-195) attributes the primary role in cellular aging to an age-dependent decrease of the passive potassium permeability of the cell membrane which is due most probably to free-radical damage of the membrane components. As a consequence, the intraceilular and intranuclear ionic strength increases resulting in a condensation of the chromatin and a slowing down of the synthetic processes performed by the nucleus. In this concept it was of importance to reveal whether the rates of total and mRNA synthesis display any age-dependent alteration parallel with the change of membrane permeability of the brain nerve cells. Experiments were performed using tritiated uridine incorporation measurements and suitable preparation techniques in young, adult and old rats (1.5, 13 and 25 mth of age, respectively). Comparisons of the incorporation rates revealed a very considerable decrease in the rate of synthesis of both the total and polyadenylated RNA (polyA + RNA) between the ages of 13 and 26 ruth. The old animals displayed only about 55 and 67% of the rate of synthesis for the 2 classes of RNA, respectively, as compared to the young and adult rats, if the results are expressed as dpm/mg RNA. However, the decreases are even more pronounced (34 and 41%) if the results are expressed on a dry weight basis. The results obtained are compatible with the membrane hypothesis of aging. membrane hypothesis of aging; mRNA synthesis in old brain; age-dependence of RNA synthesis; aging of brain

Effect of age on the activity of ceruloplasmin of human blood

Archives of Gerontology and Geriatrics, 1993

Aminoxidase activity of ceruloplasmin was measured in the serum of 120 people (between 45 and 102... more Aminoxidase activity of ceruloplasmin was measured in the serum of 120 people (between 45 and 102 years of age) using the p-phenylenediamine method. A negative linear agecorrelation (-30% for the whole lifespan; P < 0.01) was established in this activity with increasing age, nevertheless the total copper content of blood did not change in the same agerange as measured by an atomic absorption spectrophotometric method. Increasing ionic strength, in vitro, caused an exponential decline in blood ceruloplasmin aminoxidase activity of both middle aged and elderly subjects. The age-dependent decrease in ceruloplasmin activity could have a negative effect on the antioxidant functions of blood, and finally on the aging process itself.

Longevity study in Debrecen IV.: the interaction between the physical / nutritional culture and the general health status in the long-lived elderly

Debrecen longevity study II.: health and free radicals in oldest old residents

A gerontológia oktatása ma Magyarországon

Oktatási kérdések az Idősügyi Nemzeti Stratégia keretében

In vitro studies on the OH• and O2−• free radical scavenger properties of idebenone in chemical systems

Archives of Gerontology and Geriatrics, 1990

OH" free radicals were generated by Fenton reaction in the presence of bovine serum albumin (BSA)... more OH" free radicals were generated by Fenton reaction in the presence of bovine serum albumin (BSA). ]'he decreasing water-solubility of BSA with increasing Fe 2+ concentrations of the system is a sensitive indicator of the cross-linking effects of the OH" free radicals. Idebenone (oxidized form) was solubilized for this experiment in DMSO and added to the system in final concentrations of 0.01 or 0.1%. Neither of these concentrations displayed any protective effect against the insolubilization of BSA. Therefore, oxidized idebenone has to be considered as a substance which reacts with OH" free radicals slower than the BSA itself, i.e., its oxidized form is not an efficient scavenger of this type of free radicals under the given circumstances. The ability of idebenone to scavenge superoxide radicals was tested in (i) the pyrogallol system; and (ii) the xanthine-xanthine oxidase-nitro blue tetrazolium (XXO-NBT) system, ldebenone did not show any 02 " radical scavenging ability as revealed by these two in vitro methods, in the concentration ranges studied (up to 75 or 220/~g/ml, respectively). On the contrary, an increasing O~-" radical generation was observed with increasing concentrations of the drug in both test systems used. The possible biological significance of these observations is discussed in the light of other results like ESR spin trapping and measurements of superoxide dismutase (SOD) activity in various tissues.

A t(14;18) kromoszóma-transzlokáció jelenléte különbözö típusú betegségekben

Orvosi Hetilap, 1997

Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic f... more Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic follicular lymphomas. They are causative factors of lymphomas but the translocation is present in different other types of diseases although the translocation does not belong to the features of these illnesses. Our present work shows the appearance of t(14;18) translocation in lymphocytes of two patients of Sjogren's syndrome, one that of Whipple disease as well as one of healthy donors' lymphocytes using polymerase chain reaction technique presented in one of our previous publication. The translocation occurred in the mbr of bcl-2 gene in all cases showed and the bcl-2 gene was coupled with the immunoglobulin heavy chain gene. These results are definitively positive concerning the fact of translocation as it has been proved by sequencing of the amplification products showed in our earlier and present paper. Because relatively high percentages of Sjogren's syndrome patients de...

Gerontológia I-II

A Sjögren-szindróma szisztematikus megközelítése

Olvasónapló: Őszülő társadalmak

A gerontológia oktatása ma Magyarországon

Az öregedés a gerontológiai elméletek tükrében

The effects of idebenone on DNA and RNA contents as well as synthesis rates of total and poly(A)+ RNA in brain of normal, old C57BL/6J mice and in experimental partial cerebral ischemia of rats

Archives of Gerontology and Geriatrics, Nov 1, 1990

Summa~ Effects of idebenone on RNA and DNA contents as well as on synthesis rates of total and po... more Summa~ Effects of idebenone on RNA and DNA contents as well as on synthesis rates of total and poly(A) + RNA in the brain were measured in two animal models: (1) Normal young and old, male C57BL/6J mice (6 and 32 months). Idebenone suspended in 5% gum arabic was applied in 50 mg/kg/day dose to old mice for 1 month through a gastric tube. (2) Adult female CFY rats (14 18 months) in which experimental partial cerebral ischemia was induced by bilateral common carotid artery occlusion. Idebenone was administered intraperitoneally in two doses (10 mg/kg and 100 mg/kg body weight) 30 min before the interruption of carotid blood flow. DNA content remained invariate during aging in the brain; idebenone treatment did not exert any influence on this parameter. RNA content as well as total and poly(A) + RNA synthesis rates, which were measured by incorporation of tritiated uridine into RNA, decreased significantly with age in brain. Idebenone treatment did not cause any essential change of the metabolism of RNA under the given conditions. The RNA and DNA contents of brain were influenced neither by experimental partial cerebral ischemia nor by treatment with idebenone during the ischemia. Partial cerebral ischemia decreased the rate of total and poly(A) + RNA synthesis in the brain about 15-45% depending on the methods and basis of expression. This decline could totally be prevented by intraperitoneal application of 10 mg/kg idebenone 30 rain before the onset of the partial ischemia. The dose of 100 mg/kg idebenone also elevated the rate of RNA synthesis; however, this increase remained statistically insignificant.

Tissue and age specific expression of the myc proto-oncogene family throughout the life span of the C57BL/6J mouse strain

PubMed, Apr 1, 1989

The expression of the proto-oncogene myc family (c, L and N) in terms of steady-state mRNA levels... more The expression of the proto-oncogene myc family (c, L and N) in terms of steady-state mRNA levels was determined in seven different normal non-cancerous tissues throughout the life span (seven different ages) of the C57BL/6J male mouse strain. C-myc oncogene expression was highest in prenatal and newborn ages and then decreased to its lowest levels at about 6 months of age. With further increase of age, a progressive pattern of increase in expression of c-myc was found in brain, liver, skin, and small intestine. However, for kidney, spleen and heart, little or no significant change was evident. Significant differential expression of c-myc was found in most tissues in animals of the same age, with highest expression consistently being found in spleen and liver at all ages. For the N-myc and L-myc oncogenes, expression was also highest in prenatal and newborn tissue as compared to the 6-month young adult, but little or no further change was found at older ages. However, substantial tissue-dependent differences in expression were also found, and no expression at all was detected at any age for N-myc in liver and for L-myc in heart, small intestine and liver. Taken together, these results indicate that the expression of the proto-oncogenes c-, L- and N-myc is dependent not only on tissue and embryonic development, as previously shown by other workers, but also on age past the young adult stage of life span. The age-dependent increase in expression of c-myc oncogene found in normal-appearing non-cancerous tissues is of particular interest as possibly reflecting tissue alterations related to both the aging process and the age-dependent increase in cancer incidence.

One gene, two transcripts: isolation of an alternative transcript encoding for the autoantigen La/SS-B from a cDNA library of a patient with primary Sjögrens' syndrome

Journal of Experimental Medicine, Dec 1, 1994

A cDNA library was prepared from peripheral blood lymphocytes of an autoimmune patient with prima... more A cDNA library was prepared from peripheral blood lymphocytes of an autoimmune patient with primary Sj6grens' syndrome. The cDNA library was screened with the patients own autoimmune serum being monospecitic for the nuclear autoantigen La/SS-B. Thereby an alternative type of La mRNA was identified that differed from the known La mRNA due to an exchange of the exon 1. Sequencing of the genomic region between the exons 1 and 2 showed that the alternative 5'-end is a part of the intron. In addition, the presence of an alternative promoter site, which exists within the intron downstream of the exon 1, became evident. In consequence, the alternative La mRNA is the result of a promoter switching combined with an alternative splicing mechanism. In the intron, further transcription factor binding sites, including a NF-xB element, were identified leading to the suggestion that the expression of the gene encoding for the nuclear autoantigen La/SS-B alters in dependence on disease conditions.

In vivo stimulation of nerve cells by phytohemagglutinin. II. Alterations in the rate of total and mRNA synthesis in the brain cortex of old rats

Archives of Gerontology and Geriatrics, Dec 1, 1983

Summaw Bacto-phytohemagglutinin-P (PHA-P) was administered in form of a single intralumbar inject... more Summaw Bacto-phytohemagglutinin-P (PHA-P) was administered in form of a single intralumbar injection of 2 mg/100 g body weight dose to 24-to 28-mth-old female CFY rats. The accuracy of the injection technique was checked by adding 2% lidocaine to the injection mixture, which resulted in a transient and symmetric paralysis of the posterior limbs when reaching the cerebrospinal fluid. The total RNA content of the liver and brain cortex were measured, and phenolic extraction of RNA was performed on the brain cortex. Poly(A) + RNA (mRNA) was separated from the total RNA of the brain cortex by oligo(dT)-cellulose chromatography. Pulse labeling with tritiated uridine was performed 45 min before killing the animals and the incorporation of the radiolabel was measured in the respective RNA classes and corrected for the nucleotide pool size. The rates of total and mRNA synthesis are expressed in percentages of the young untreated rats and compared to old untreated animals. The effects of PHA-P was studied at 4, 10, 20 and 44 h after its injection. A considerable increase of the total RNA content of the brain cortex was measured during the first 10 h of the experiment followed by a slow decrease. However, the RNA content of the brain cortex was still significantly higher at the end of experiment compared to untreated old rats. The rate of total RNA synthesis increased significantly during the first 10 h and remained constantly high until 44 h. The rate of mRNA synthesis increased to a higher extent than that of the total RNA, and also remained high until 44 h. membrane hypothesis of aging; phytohemagglutinin; aging of brain cells; rate of RNA synthesis; rate of mRNA synthesis

Centrophenoxine increases the rates of total and mRNA synthesis in the brain cortex of old rats: An explanation of its action in terms of the membrane hypothesis of aging

Experimental Gerontology, 1984

The rates of total and polyA + RNA (mRNA) synthesis were measured by radioisotope technique in th... more The rates of total and polyA + RNA (mRNA) synthesis were measured by radioisotope technique in the brain cortex of female CFY rats. There was practically no significant difference between the young (1.5 months) and adult (13 months) rats; however, the old group (26 months) displayed a considerable decrease of the rates of synthesis of both classes of RNA studied. Centrophenoxine treatment (100 mg per kg body weight per day, for 2 months) reversed this tendency, and increased significantly the synthesis rates of old rats almost to the adult level. The results are interpreted in terms of the membrane hypothesis of aging, attributing a free-radical scavenger function of the dimethylamino-ethanol incorporated into the nerve cell membrane from the centrophenoxine.

Effects of ionic strength on the activity of superoxide dismutase in vitro

Archives of Gerontology and Geriatrics, Dec 1, 1984

Changes in superoxide dismutase (SOD) activity were studied in vitro at increasing NaC1 or KC1 co... more Changes in superoxide dismutase (SOD) activity were studied in vitro at increasing NaC1 or KC1 concentrations. SOD activity was measured using two different systems of superoxide radical generation: pyrogallol autoxidation, and xanthine-xanthine oxidase reaction. Pyrogallol autoxidation was directly measured by spectrophotometry, whereas in the second case cytochrome c reduction was followed at 550 nm. The inhibition of SOD on those parameters was taken as measure of SOD activity. Increasing concentrations of NaCl and KC1 significantly increased the rate of pyrogallol autoxidation. The inhibitory effect of SOD significantly decreased under the influence of these salts and followed an exponential curve. The two salts studied resulted in essentially identical changes in SOD activity. Increasing concentrations of NaC1 and KCI decreased the rate of cytochrome c reduction in the xanthine-xanthine oxidase system. When correcting the results for these primary effects, SOD activity also displayed in this system an exponential decay with increasing salt concentrations. The results are interpreted in terms of the known charge distribution pattern on the surface of the SOD molecule, and of the age-dependent increase of the intracellular potassium and sodium concentrations in the postmitotic cells. superoxide dismutase; ionic strength dependence of SOD activity; intracellular ionic composition during aging; pyrogallol autoxidation for measuring SOD activity; xanthine-xanthine oxidase reaction; cytochrome c reduction

[The presence of t(14;18) chromosome translocation in various types of diseases]

PubMed, Dec 28, 1997

Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic f... more Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic follicular lymphomas. They are causative factors of lymphomas but the translocation is present in different other types of diseases although the translocation does not belong to the features of these illnesses. Our present work shows the appearance of t(14;18) translocation in lymphocytes of two patients of Sjögren's syndrome, one that of Whipple disease as well as one of healthy donors' lymphocytes using polymerase chain reaction technique presented in one of our previous publication. The translocation occurred in the mbr of bcl-2 gene in all cases showed and the bcl-2 gene was coupled with the immunoglobulin heavy chain gene. These results are definitively positive concerning the fact of translocation as it has been proved by sequencing of the amplification products showed in our earlier and present paper. Because relatively high percentages of Sjögren's syndrome patients develop later on lymphoma, the early detection of the translocation could result in a more successful diagnosis as well as treatment of the disease. The question arises, however, what role the translocation plays in illnesses such as the Whipple disease or what kind of consequences can be drawn from the appearance of the t(14;18) translocation in lymphocytes of healthy donors.

In vivo studies on the age-dependent decrease of the rates of total and mRNA synthesis in the brain cortex of rats

Archives of Gerontology and Geriatrics, 1982

The membrane hypothesis of aging (Zs.-Nagy, I., 1978, J. Theor. Biol. 75, 189-195) attributes the... more The membrane hypothesis of aging (Zs.-Nagy, I., 1978, J. Theor. Biol. 75, 189-195) attributes the primary role in cellular aging to an age-dependent decrease of the passive potassium permeability of the cell membrane which is due most probably to free-radical damage of the membrane components. As a consequence, the intraceilular and intranuclear ionic strength increases resulting in a condensation of the chromatin and a slowing down of the synthetic processes performed by the nucleus. In this concept it was of importance to reveal whether the rates of total and mRNA synthesis display any age-dependent alteration parallel with the change of membrane permeability of the brain nerve cells. Experiments were performed using tritiated uridine incorporation measurements and suitable preparation techniques in young, adult and old rats (1.5, 13 and 25 mth of age, respectively). Comparisons of the incorporation rates revealed a very considerable decrease in the rate of synthesis of both the total and polyadenylated RNA (polyA + RNA) between the ages of 13 and 26 ruth. The old animals displayed only about 55 and 67% of the rate of synthesis for the 2 classes of RNA, respectively, as compared to the young and adult rats, if the results are expressed as dpm/mg RNA. However, the decreases are even more pronounced (34 and 41%) if the results are expressed on a dry weight basis. The results obtained are compatible with the membrane hypothesis of aging. membrane hypothesis of aging; mRNA synthesis in old brain; age-dependence of RNA synthesis; aging of brain

Effect of age on the activity of ceruloplasmin of human blood

Archives of Gerontology and Geriatrics, 1993

Aminoxidase activity of ceruloplasmin was measured in the serum of 120 people (between 45 and 102... more Aminoxidase activity of ceruloplasmin was measured in the serum of 120 people (between 45 and 102 years of age) using the p-phenylenediamine method. A negative linear agecorrelation (-30% for the whole lifespan; P < 0.01) was established in this activity with increasing age, nevertheless the total copper content of blood did not change in the same agerange as measured by an atomic absorption spectrophotometric method. Increasing ionic strength, in vitro, caused an exponential decline in blood ceruloplasmin aminoxidase activity of both middle aged and elderly subjects. The age-dependent decrease in ceruloplasmin activity could have a negative effect on the antioxidant functions of blood, and finally on the aging process itself.

Longevity study in Debrecen IV.: the interaction between the physical / nutritional culture and the general health status in the long-lived elderly

Debrecen longevity study II.: health and free radicals in oldest old residents

A gerontológia oktatása ma Magyarországon

Oktatási kérdések az Idősügyi Nemzeti Stratégia keretében

In vitro studies on the OH• and O2−• free radical scavenger properties of idebenone in chemical systems

Archives of Gerontology and Geriatrics, 1990

OH" free radicals were generated by Fenton reaction in the presence of bovine serum albumin (BSA)... more OH" free radicals were generated by Fenton reaction in the presence of bovine serum albumin (BSA). ]'he decreasing water-solubility of BSA with increasing Fe 2+ concentrations of the system is a sensitive indicator of the cross-linking effects of the OH" free radicals. Idebenone (oxidized form) was solubilized for this experiment in DMSO and added to the system in final concentrations of 0.01 or 0.1%. Neither of these concentrations displayed any protective effect against the insolubilization of BSA. Therefore, oxidized idebenone has to be considered as a substance which reacts with OH" free radicals slower than the BSA itself, i.e., its oxidized form is not an efficient scavenger of this type of free radicals under the given circumstances. The ability of idebenone to scavenge superoxide radicals was tested in (i) the pyrogallol system; and (ii) the xanthine-xanthine oxidase-nitro blue tetrazolium (XXO-NBT) system, ldebenone did not show any 02 " radical scavenging ability as revealed by these two in vitro methods, in the concentration ranges studied (up to 75 or 220/~g/ml, respectively). On the contrary, an increasing O~-" radical generation was observed with increasing concentrations of the drug in both test systems used. The possible biological significance of these observations is discussed in the light of other results like ESR spin trapping and measurements of superoxide dismutase (SOD) activity in various tissues.

A t(14;18) kromoszóma-transzlokáció jelenléte különbözö típusú betegségekben

Orvosi Hetilap, 1997

Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic f... more Chromosome translocation of t(14;18) can be detected in most cases of centroblastic/centrocytic follicular lymphomas. They are causative factors of lymphomas but the translocation is present in different other types of diseases although the translocation does not belong to the features of these illnesses. Our present work shows the appearance of t(14;18) translocation in lymphocytes of two patients of Sjogren's syndrome, one that of Whipple disease as well as one of healthy donors' lymphocytes using polymerase chain reaction technique presented in one of our previous publication. The translocation occurred in the mbr of bcl-2 gene in all cases showed and the bcl-2 gene was coupled with the immunoglobulin heavy chain gene. These results are definitively positive concerning the fact of translocation as it has been proved by sequencing of the amplification products showed in our earlier and present paper. Because relatively high percentages of Sjogren's syndrome patients de...

Gerontológia I-II

A Sjögren-szindróma szisztematikus megközelítése

Olvasónapló: Őszülő társadalmak

A gerontológia oktatása ma Magyarországon

Az öregedés a gerontológiai elméletek tükrében