Mayank Gangwar

Banaras hundu university, Pharmacology, Senior Research Fellow-CSIR

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Phone: +919458429841
Address: Dept of Pharmacology, Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi- 221005 (India)

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Papers by Mayank Gangwar

Effect of a Novel Ashwagandha-based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocyte Cells: A Potential Immunomodulator

Background: Herbomineral formulations are momentous in an audience of worldwide by virtue of thei... more Background: Herbomineral formulations are momentous in an audience
of worldwide by virtue of their holistic approach to life. These formulations
are widely used as complementary therapies in immunocompromised
patients including cancer. Still, there is the need of cost-effective and safe
herbomineral-based formulation that can modulate immune response by the
regulation of cytokines cascades. Objective: Current study, we investigated
immunomodulatory effect of TEBEH in LPS-induced cytokines expression
levels in mouse splenocytes in vitro. Materials and Methods: The most
effective and safe concentrations of TEBEH were chosen by determining
the cell viability of splenocytes using MTT assay. The pro-inflammatory
cytokines such as TNF-α, IL-1β, MIP-1α, and IFN-γ were measured in cell
supernatants using ELISA. Results: MTT data showed TEBEH formulation
was found safe up to 10.53 μg/mL. At noncytotoxic concentrations
(0.00001053–10.53 μg/mL), TEBEH significantly (P ≤ 0.001) inhibited the
expressions of TNF-α, IL-1β, and MIP-1α in mouse splenocytes as compared
with vehicle control. Conclusion: In summary, TEBEH may indeed promote
an anti-inflammatory environment by suppression of pro-inflammatory
cytokines. These observations indicated that TEBEH has potential effects
in downregulating the immune system and might be developed as a useful
anti-inflammatory product for various inflammatory disorders.

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Alternative & Integrative Medicine

ABSTRACT

Qualitative phytochemical and physiochemical analysis of Cassia fistula L. fruit

Medicinal Plants International Journal of Phytomedicines and Related Industries, 2014

Pharmacological evaluation of Mallotus philippinensis (Lam.) Muell.-Arg. fruit hair extract for Anti-Inflammatory, Analgesic and Hypnotic Activity

Journal of Intercultural Ethnopharmacology, 2016

Recently, we observed wound healing activity of 50% ethanol extract of Mallotus philippinensis Mu... more Recently, we observed wound healing activity of 50% ethanol extract of Mallotus philippinensis Muell. Arg (MP) fruit hairs extract (MPE). In several intestinal infections, localized inflammation is of common occurrence and hence we evaluated the anti-inflammatory, analgesic and hypnotic activity of MPE in different rat experimental models. Anti-inflammatory activity was evaluated by carrageenan (acute) and turpentine oil induced formalin (subacute) induced paw edema and while granuloma pouch (subacute) in rats. Analgesic and hypnotic activity of MPE was undertaken by tail-flick, hot-plate, and acetic acid-induced writhing tests while pentobarbitone-induced hypnotic potentiation in rats. MPE at a dose of 200 mg/kg at 3 h after their administration showed inhibition of formalin-induced paw edema by 41.60% (P &amp;amp;amp;amp;amp;amp;lt; 0.001) and carrageenan-induced paw edema by 55.30% (P &amp;amp;amp;amp;amp;amp;lt; 0.001). After 7 days of treatments, MPE showed 38.0% (P &amp;amp;amp;amp;amp;amp;lt; 0.001) inhibition against formalin-induced paw edema and reduced weight of turpentine-induced granuloma pouch by 29.6% (P &amp;amp;amp;amp;amp;amp;lt; 0.01) and volume of exudates by 26.1% (P &amp;amp;amp;amp;amp;amp;lt; 0.01), respectively. MPE (200 mg/kg) showed dose-dependent elevation in pain threshold and peak analgesic effect at 120 min as evidenced by increased latency period in tail flick method and increased reaction time in the hot-plate test while the reduction in the number of acetic acid-induced writhes by 45.7% (P &amp;amp;amp;amp;amp;amp;lt; 0.001). The pentobarbitone-induced hypnosis model showed potentiation, as defined by increased duration of sleep in treated group rats as compared to control. Thus, the study revealed MPE is effective in reducing acute and subacute inflammation and showed effective and similar analgesic activity. This seemed to be safe in the treatment of pain and inflammation.

Qualitative characterization of phytochemicals and in vitro antimicrobial evaluation of leaf extract of couroupita guianensis aubl. - A threatened medicinal tree

International Journal of Pharmacy and Pharmaceutical Sciences

ChemInform Abstract: Chemical Constituents of Anisodus luridus Roots and Their Antimicrobial Screening

ChemInform, 2015

Design, Synthesis and Mode of Action of some new 2-(4'-aminophenyl)benzothiazole Derivatives as Potent Antimicrobial Agents

Letters in Drug Design & Discovery, 2015

Synthesis, DNA cleavage and antimicrobial activity of 4-thiazolidinones-benzothiazole conjugates

Indian journal of experimental biology

Antimicrobial screening of several novel 4-thiazolidinones with benzothiazole moiety has been per... more Antimicrobial screening of several novel 4-thiazolidinones with benzothiazole moiety has been performed. These compounds were evaluated for antimicrobial activity against a panel of bacterial and fungal strains. The strains were treated with these benzothiazole derivatives at varying concentrations, and MIC's were calculated. Structures of these compounds have been determined by spectroscopic studies viz., FT-IR, 1H NMR, 13C NMR and elemental analysis. Significant antimicrobial activity was observed for some members of the series, and compounds viz. 3-(4-(benzo[d]thiazol-2-yl) phenyl-2-(4-methoxyphenyl)thiazolidin-4-one and 3-(4-(benzo[d]thiazol-2-yl)phenyl)-2-(4-hydroxy phenyl)thiazolidin-4-one were found to be the most active against E.coli and C. albicans with MIC values in the range of 15.6-125 microg/ml. Preliminary study of the structure-activity relationship revealed that electron donating groups associated with thiazolidine bearing benzothiazole rings had a great effect ...

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Phytochemical and physiochemical analysis of Cassia fistula L. fruit

The fruit pulp of Cassia fistula Linn. is known to have purgative properties. A specific process,... more The fruit pulp of Cassia fistula Linn. is known to have purgative properties. A specific process, i.e., keeping well ripe fruits buried within sand for seven days and then drying in sun prior to its medicinal use, has been mentioned in Charaka Samhita. To explore the effect of above classical processing, a comparative physiochemical and phytochemical evaluation of classically processed (PD) and non-processed (NPD) fruit pulp of Cassia fistula has been done together with HPTLC. Results of the study showed presence of alkaloid, glycosides, steroids, triterpenes, phenolic compounds, tannins, proteins, amino acids and carbohydrats in both types of samples. The extractive values of PD and NPD were 18.25 % w/v and 15.65% w/v, respectively. The difference in physiochemical analysis of PD and NPD was also recorded and the HPTLC of PD sample showed an extra peak around Rf 0.76 in comparison to NPD. These results signify appreciable changes in classically processed sample of Cassia fistula fr...

Effects of Azardirachta indica on Vascular Endothelial Growth Factor and Cytokines in Diabetic Deep Wound

Planta Medica, 2015

A chronic, unhealed diabetic wound is one of the severe complications of diabetes mellitus. Azadi... more A chronic, unhealed diabetic wound is one of the severe complications of diabetes mellitus. Azadirachta indica has been reported to have antidiabetic and antiapoptotic properties. The present work incorporates the healing potential of 50 % ethanol A. indica leaves extract against deep surgical wounds in streptozotocin-induced mild diabetic rats. A. indica leaves extract (500 mg/kg) was administered orally, once daily for ten days. Serum glucose, cholesterol, and triglycerides as well as body weight, food, and water intake, and tissue antioxidants (catalase, superoxide dismutase and reduced glutathione), free radicals (lipid peroxidation and nitric oxide), myeloperoxidase, total collagens (hydroxyproline, hexuronic acid and hexosamine), protein, vascular endothelial growth factor, and cytokines (tumor necrotic factor-α and interleukin-1β) were estimated. Histology was done for connective tissue formation and inflammatory and healing in deep granulation tissue after A. indica leaves extract treatment. Diabetic rats showed an increase in serum glucose, cholesterol, and triglycerides levels, food and water intake, and granular tissue free radicals, myeloperoxidase, and cytokines, but a decrease in body weight, total collagen, and vascular endothelial growth factor levels. A. indica leaves extract reversed the increased serum glucose, cholesterol, and triglycerides, food and water intake, and tissue free radicals, myeloperoxidase and, cytokines, but increased body weight, tissue antioxidants, total collagen, and vascular endothelial growth factor contents. The results thus indicated an improvement in wound healing by A. indica leaves extract in diabetic rats through enhanced angiogenesis mediated through the inhibition of hyperglycemia, oxidative stress, and down- and upregulation of inflammatory mediators and growth factor expression.

Mallotus philippinensis Muell. Arg fruit glandular hairs extract promotes wound healing on different wound model in rats

BMC Complementary and Alternative Medicine, 2015

Antimicrobial Activity of Phytochemicals Isolated from Selaginella bryopteris

Chemistry of Natural Compounds, 2015

ABSTRACT The heterosporous fern genus Selaginella (Selaginellaceae) consists of more than 700 spe... more ABSTRACT The heterosporous fern genus Selaginella (Selaginellaceae) consists of more than 700 species [1] and grows mainly in warm and moist climates with a worldwide distribution. More than 62 Selaginella species were reported from India [2, 3]. The genus Selaginella is a rich source of biflavonoids, which are dimers of flavonoids linked by a C-O-C or C-C bond [4]. Studies have shown that the genus Selaginella is a rich source of steroids [5], biflavonoids [6], alkaloids [7], secolignans [8], neolignans, and caffeoyl derivatives [9]. In the traditional system of Indian medicine, S. bryopteris is used as a tonic for regeneration of energy and vitality [10]. Extracts of S. bryopteris were shown to have antioxidant [11], anticarcinogenic [12], antiplasmodial, and leishmanicidal activity [6].In the present study, systematic chemical investigation of the whole plant of S. bryopteris was done, which lead to the isolation and characterization of a sterol, a lignan, two biflavonoids, a phenolic compou ...

Mallotus philippinensis Muell. Arg (Euphorbiaceae): Ethnopharmacology and Phytochemistry Review

BioMed Research International, 2014

Mallotus philippinensis Muell. Arg (Euphorbiaceae) are widely distributed perennial shrub or smal... more Mallotus philippinensis Muell. Arg (Euphorbiaceae) are widely distributed perennial shrub or small tree in tropical and subtropical region in outer Himalayas regions with an altitude below 1,000 m and are reported to have wide range of pharmacological activities. Mallotus philippinensis species are known to contain different natural compounds, mainly phenols, diterpenoids, steroids, flavonoids, cardenolides, triterpenoids, coumarins, isocoumarins, and many more especially phenols; that is, bergenin, mallotophilippinens, rottlerin, and isorottlerin have been isolated, identified, and reported interesting biological activities such as antimicrobial, antioxidant, antiviral, cytotoxicity, antioxidant, anti-inflammatory, immunoregulatory activity protein inhibition against cancer cell. We have selected all the pharmacological aspects and toxicological and all its biological related studies. The present review reveals that Mallotus philippinensis is a valuable source of medicinally important natural molecules and provides convincing support for its future use in modern medicine. However, the existing knowledge is very limited about Mallotus philippinensis and its different parts like steam, leaf, and fruit. Further, more detailed safety data pertaining to the acute and subacute toxicity and cardio- and immunotoxicity also needs to be generated for crude extracts or its pure isolated compounds. This review underlines the interest to continue the study of this genus of the Euphorbiaceae.

Effect of Mangifera Indica Seed Kernel Extract on Rat Wound Healing

Osmium tetroxide catalysed oxidation of sorbitol and mannitol with hexacyanoferrate (III) in aqueous alkaline medium

Synthesis, DNA cleavage and antimicrobial activity of 4-thiazolidinones-benzothiazole conjugates

Indian J Exp Biol, 2014

Qualitative phytochemical and physiochemical analysis of Cassia fistula L. fruit

Medicinal Plants - International Journal of Phytomedicines and Related Industries, 2014

In-vitro scolicidal activity of Mallotus philippinensis (Lam.) Muell Arg. fruit glandular hair extract against hydatid cyst Echinococcus granulosus

Asian Pacific Journal of Tropical Medicine, 2013

To investigate new scolicidal agent from natural resources to cope with the side effects associat... more To investigate new scolicidal agent from natural resources to cope with the side effects associated with synthetic drugs in Echinococcosis. The scolicidal potential of methanolic fruit powder extract (10 and 20 mg/mL) of Mallotus philippinensis (M. philippinensis) was investigated. Viability of protoscoleces was confirmed by trypan blue exclusion method, where mortality was observed at concentration of 10 and 20 mg/mL in 60 min treatment against Echinococcus granulosus (E. granulosus), under in-vitro conditions with reference to the known standard drug Praziquantel®. At concentration 10 and 20 mg/mL, the mortality rate was observed 97% and 99% respectively for 60 min treatment; while up to 93% mortality was observed with 20 mg/mL for only 10 min treatment. The concentration above 20 mg/mL for above 2 h showed 100% mortality, irrespective of further incubation. As compared with the standard anti-parasitic drug Praziquantel our extract has significant scolicidal activity with almost no associated side effects.

Engineered Nanomaterials for Purification and Desalination of Palatable Water

Kharisov/Nanomaterials for Environmental Protection, 2014

ABSTRACT

Anticestodal Activity of Endophytic Pestalotiopsis sp. on Protoscoleces of Hydatid Cyst Echinococcus granulosus

BioMed Research International, 2013

Surgery is still the main treatment in hydatidosis caused by Echinococcus, which is a global heal... more Surgery is still the main treatment in hydatidosis caused by Echinococcus, which is a global health problem in human and animals. So, there is need for some natural protoscolicidal agents for instillation to prevent their reoccurrence at therapeutic doses. In this present investigation, anticestodal activity of one of the endophytic fungi Pestalotiopsis sp. from Neem plant was observed on protoscoleces of hydatid cysts of Echinococcus granulosus. Viability of protoscoleces was confirmed by 0.1% aqueous eosin red stain method, where mortality was observed at different concentrations with respect to time. An average anticestodal activity was observed with different endophytic fungal strains, that is, Nigrospora (479 ± 2.9), Colletotrichum (469 ± 25.8), Fusarium (355 ± 14.5), and Chaetomium (332 ± 28.3) showing 64 to 70% protoscolicidal activity, except Pestalotiopsis sp. (581 ± 15.0), which showed promising scolicidal activity up to 97% mortality just within 30 min of incubation. These species showed significant reduction in viability of protoscoleces. This is the first report on the scolicidal activity of endophytic Pestalotiopsis sp. We conclude that ultrastructural changes in protoscoleces were due to endophytic extract suggesting that there may be some bioactive compounds that have selective action on the tegument layer of protoscoleces. As compared with that of standard drug used, endophytic species of Neem plant shows significant anticestodal activity.

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Effect of a Novel Ashwagandha-based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocyte Cells: A Potential Immunomodulator