β-Glucan is an essential component of the cell walls of grains such as oats and barley. 1,3-1,4-β... more β-Glucan is an essential component of the cell walls of grains such as oats and barley. 1,3-1,4-β-D-glucan 4-glucanhydrolase (β-glucanase or lichenase) (EC 3.2.1.73) is an enzyme with the ability to hydrolyze β-glucans. In this research, β-Glucan which is a good source of feed additive fi sh probiotics, was used in order to benefi t from feed quality in fi shery products, to increase live weight gain and to strengthen the immune system. In this study, recombinant vector pNW33N carrying the β-(1,3-1,4) glucanase (lichenase) gene of Streptococcus bovis genome was transferred to Bacillus subtilis RSKK246 (CMCase+) strain by electroporation. Subsequently, electrotransformation was performed on LB-agar plates containing lichenan and enzymatic activity regions of recombinant B. subtilis RSKK246 colonies were observed by staining with Congo red. In addition, the DNA from the recombinant plasmid pNW33N+Lichenase (pNW33NLic) was cut on both the BamHI and HindIII endonucleases and observed on the lichenase gene (1800 bp) agarose gel. On the other hand, the protein band corresponding to 26 kDa of the recombinant enzyme was observed by zymogram analysis. These results indicate that the β-(1,3-1,4) glucanase gene has been successfully expressed to the B. subtilis strain RSKK246.
Giriş: Silaj, yeşil yem bitkilerinin anaerobik koşullar altında fermente edilerek saklanmasıyla e... more Giriş: Silaj, yeşil yem bitkilerinin anaerobik koşullar altında fermente edilerek saklanmasıyla elde edilen, besin madde bakımından oldukça zengin ve tercih edilebilir bir kaba yem kaynağıdır. Silaj açıldıktan sonra hava ile teması sonucu, silaj aerobik küf ve maya kontaminasyonuna maruz kalmakta ve sonuçta ciddi ekonomik kayıplar ve sağlık açısından riskler ortaya çıkmaktadır. Bu çalışmada Cellulosimicrobium cellulans’a ait β-1,3-glukanaz geninin Lactobacillus plantarum’da klonlanması ile aerobik bozulmayı önleyen rekombinant silaj inokülantının geliştirilmesi amaçlanmıştır. Gereçler ve Yöntem: Bu çalışmada β-1,3-glukanaz gen kaynağı olarak C. cellulans bakterisi kullanılmıştır. Gen PCR yöntemi ile bakterinin genomundan amplifiye edilmiştir. Klonlama vektörleri olarak pUC18 ve pTE353-2 plazmit DNA’ları kullanılmıştır. Geni taşıyan rekombinant plazmit DNA’ların Escherichia coli ve L. plantarum’a aktarılmasında sırasıyla CaCl2 transformasyonu ve elektroporasyon teknikleri kullanılmış...
Lysozyme (EC.3.2.1.17) is an enzyme that catalyses the hydrolysis of beta-1,4 glucosidic linkages... more Lysozyme (EC.3.2.1.17) is an enzyme that catalyses the hydrolysis of beta-1,4 glucosidic linkages between N-acetyl glucosamine (NAG) and N-acetyl muramic acid (NAM) in the peptidoglucan heteropolymers of prokaryotic cell walls. In this study, lysozyme enzyme found in chicken egg white was treated under different pH conditions (pH 4.0, 6.0, 8.0, 10.0, 12.0) and the results were observed as electrophoretic. Electrophoretic results revealed that egg white lysozyme was best yielded at pH 4.0. As a result of dropping the egg white solution on the Bacillus subtilis spreaded on the LB-agar plates with a glass rod, it was observed that the bacteria could not developed due to the lysozyme of egg white. On the other hand, genomic and plasmid DNAs were isolated from the bacteria in DNA isolation studies in which the egg white solution was replaced by commercial lysozyme. The results showed that, in the absence of commercial lysozyme under laboratory conditions, chicken egg white can be used as...
Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi
To obtain high efficiency from animals, it is necessary to maximize the ability to benefit from f... more To obtain high efficiency from animals, it is necessary to maximize the ability to benefit from feed, as well as to protect animal health. One of the important applications in this direction is feed additives. Feed additives are plant and animal products and microorganisms that increase fish production and welfare, improve digestion and digestive system microflora and contribute to the preservation of nutrients and feed. Although plant and animal tissues are used in enzyme production, the use of microorganisms is in the first place. After the use of molecular genetic techniques, the genes responsible for enzyme production were cloned in microorganisms, making it possible to produce enzymes more purely and cheaply on an industrial scale.
Bu çalışmayla, tütsülenmiş ve marine edilmiş gökkuşağı alabalığının (Oncorhynchus mykiss) buzdola... more Bu çalışmayla, tütsülenmiş ve marine edilmiş gökkuşağı alabalığının (Oncorhynchus mykiss) buzdolabında depolama süresince elektroforetik protein proflindeki değişimlerin belirlenmesi amaçlanmıştır. Çiğ, tütsülenmiş ve marine edilmiş alabalık örneklerinin , 9 haftalık buzdolabında depolama süresince kas proteinlerindeki değişimler sodyum dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE) kullanılarak incelenmiştir. SDS-PAGE ve densitometrik analiz sonuçlarına göre, tütsülenmiş ve marine edilmiş alabalık kaslarının, aktin bandlarında önemli bir değişimin olmadığı, buna karşılık bazı protein banlarının sayısında ve yoğunluğunda bir azalmanın meydana geldiği gözlemlenmiştir. Depolamanın 9. haftasında, 205 kDa molekül ağırlığındaki myosin ağır zinciri (MHC), gözden kaybolmuştur. Ayrıca tütsülenmiş ve marine edilmiş örneklerin protein yüzdeleri de haftalık olarak önemli oranda değişim göstermiştir (P<0.01). Çiğ, tütsülenmiş ve marine edilmiş örneklerin protein yüzdeleri sırasıyl...
In this study, serin esterase gene from Spirulina platensis amplified by
PCR using primer sequen... more In this study, serin esterase gene from Spirulina platensis amplified by
PCR using primer sequences. The amplified gene cloned into the pBR325 vector,
thus, entegrative vectors for S. platensis were constructed. The new entegrative
vector were identified as pBR325SE2. Bacteriophage T4 lysosyme gene was
cloned into pBR325SE2 and the new vector harbouring lysozyme gene was
identified as pBR325SL. pBR325SL was transferred into competent E. coli and S.
platensis respectively. Recombinant S. platensis/pBR325SL grown in the Spirulina
medium with cml while there was no non-transformant Spirulina in the same
medium but intensive non-transformant growth was observed in the medium
without cml. While the presence of lysozyme gene was demonstrated in pBR325SL
isolated from E. coli by PCR, but it couldn’t be demonstrated in S.
platensis/pBR325SL because of insufficient Spirulina in the medium with cml
growth for DNA isolation
TEZ6224Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2007.Kaynakça (s.72-76) var.xiii, 84 s. : r... more TEZ6224Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2007.Kaynakça (s.72-76) var.xiii, 84 s. : rnk. ; 29 cm.In this study, serin esterase gene from Spirulina platensis amplified by PCR using two different primer sequences. The amplified gene cloned into the pBR325 vector in two different way, depending on differences between primers, thus, two entegrative vectors for S. platensis were constructed. The new entegrative vectors were identified as pBR325SE1 and pBR325SE2 respectively. While pBR325SE1 and pBR325SE2 were transferred into competent E. coli, besides this, pBR325SE1 was electroporated into S. platensis. Recombinant S. platensis/pBR325SE1 were grown in the Spirulina medium with chloramphenicol (cml) while there wasn't any non-transformant Spirulina in the same medium but intensive non-transformant growth was observed in the medium without cml. Bacteriophage T4 lysosyme gene was cloned into pBR325SE2 and the new vector harbouring lysozyme gene was identified as pBR325SL. ...
The aim of this study was to compare Bayesian and Classical estimation for describing the growth ... more The aim of this study was to compare Bayesian and Classical estimation for describing the growth curves of red mullet (Mullus barbatus barbatus Linnaeus, 1758). Classical nonlinear regression method and Bayesian estimation method were used to obtain the estimation of the components of the von Bertalanffy growth model. The estimated parameters showed that Bayesian estimation is better than Classical nonlinear regression in estimating growth parameters and reducing variation of growth model parameters.
Objective: This study presents 660 length-weight relationships gathered from literature related t... more Objective: This study presents 660 length-weight relationships gathered from literature related to 225 fish species, throughout Turkish marine waters. Methods: All length-weight relationships presented here are the product of published 27 length-weight relationship studies conducted during 1997-2011 in Turkish marine waters. Summary of length-weight relationship parameters (a and b), was presented and both of regression parameters was compared with genus, season and study area using analysis of variance (ANOVA) and analysis covariance (ANCOVA). Correlation between parameters a and b was calculated across species. Results and Discussion: Overall, 13 out of the 660 length-weight relationships refer to Mullus barbatus (2%), 12 (1.8%) to Diplodus annularis and 12 (1.8%) to Arnoglossus laterna. In terms2.9%) relationships, respectively. Most r 2 values being greater than 0.80 (28 of 660 relationships were smaller than 0.80). Across species mean of b (3.016) is not significantly larger th...
Abstract The aim of this study was to determine the effects of different cooking methods on the e... more Abstract The aim of this study was to determine the effects of different cooking methods on the electrophoretic patterns of rainbow trout (Oncorhynchus mykiss) fillets using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Raw rainbow trout were ...
In order to put cultured species on the market with high quality and few casualties, many importa... more In order to put cultured species on the market with high quality and few casualties, many important studies are carried out. Most of the researches are conducted in the development of feed and feed ingredients 30-60% of the production cost in farming. Therefore, in aquaculture, an interest in alternative feed ingredients is moving at a very fast rate. In this context, the use of enzymes, probiotics and prebiotics in animal feed has steadily increased in recent years with reasons such as effective control of fish diseases and prevention of infection, strengthening the immune system of fish, increase of the digestibility, reduction of the feed cost, reduction of larval-term mortality, provision of increase in growth, live weight gain, and getting rid of the negative effects of stress.
DESCRIPTION Türkiye’nin Akdeniz kıyılarında yayılış gösteren 26 lesepsiyen türe ait yayınlanmış 1... more DESCRIPTION Türkiye’nin Akdeniz kıyılarında yayılış gösteren 26 lesepsiyen türe ait yayınlanmış 11 çalışmadan toplam 63 boy ağırlık ilişkisi Google Scholar, Academia edu, Researchgate ve Scopus gibi veri tabanı/arama motorlarından elde edilmiş ve incelenmiştir.
The aim of this study is to compare Bayesian and Classical estimation for describing the growth c... more The aim of this study is to compare Bayesian and Classical estimation for describing the growth curves of Brushtooth Lizardfish (Saurida lessepsianus Russell, Golani & Tikochinski 2015). Classical nonlinear regression method and Bayesian estimation method were used to obtain the estimation of the components of the von Bertalanffy growth model. The estimated parameters of the von Bertalanffy equation via two methods showed that Bayesian estimation is much better than Classical nonlinear regression in estimating growth parameters and reducing variation of growth model parameters.
Spirulinaisphotosynthetic,�filamentous,� spiral-shaped,�multicellularandgreen-bluemicroalga.�Thet... more Spirulinaisphotosynthetic,�filamentous,� spiral-shaped,�multicellularandgreen-bluemicroalga.�Thetwomostimportantspeciesofwhichare� Spirulina maxima and Spirulina platensis. Since its chemical composition includes proteins, carbohydrates, vitamins, minerals, essential fatty acids andpigmentslikechlorophylla, carotenes and phycocyanin, it is considered an excellent food and feed. It also used in medicine and cosmetic industries.�Becauseofitsnutritional,�medicalandbiologicalvalue,�geneticstudiesonSpirulinahavebeenincreasedallovertheworldtodevelop� newstrainsgainednewproperties.
The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of th... more The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in two tilapia species, Oreochromis aureus and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435 and 342 bp, respectively) were amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate restriction endonuclease enzymes and analysed using agarose gel electrophoresis. Link of the (Abstract) Paper: https://www.wflpublisher.com/Abstract/3998 Link of the website: https://www.wflpublisher.com
This study aimed to determine the effects on protein of frozen storage time (-20 °C), and on prop... more This study aimed to determine the effects on protein of frozen storage time (-20 °C), and on properties of raw, mar-inated and smoked rainbow trouts (Oncorhynchus mykiss). Therefore, the fish were smoked and marinated. All filleted fish samples including also the raw ones were vacuum-packed, subjected to 9 months of frozen storage and analyzed at 1-month intervals. Changes in muscle proteins of raw, smoked and marinated trout samples (Oncorhynchus mykiss) have been examined using sodium dodecyl sul-phate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE and densitometric analysis revealed that intensity and the number of protein bands were not changed until the 9 th month of raw and marinating process while the bands of some protein patterns denaturated when trout muscle was subjected to 9 months of smoking process. My-osin heavy chain (MHC) band with molecular weights of 205 kDa and actin band with molecular weights of 45 kDa appeared during 9 months of raw, smoking and marinating process. Similarly, other main myofibrillar proteins, tropo-myosin (37.7 kDa), troponin-T (32.9 kDa), myosin light chain 1 (27.2 kDa), and myosin light chain 2 (20.4 kDa) also did not show any differences during 9-months storage. Subsequently, there were significant monthly changes in the protein percentages caused by smoking and marinating processes excluding the raw ones (P&lt;0.01).
Heritability estimates were determined for six phenotypes in Oreochromis niloticus for 180, 210 a... more Heritability estimates were determined for six phenotypes in Oreochromis niloticus for 180, 210 and 300 days old: body weight (BW), total length (TL), standard length (SL), body height (BH), caudal tail height (CTH) and head length (HL). Heritability estimates were calculated by half-sib analysis from an experiment that contained 3 paternal half-sib families and 11 full-sib families. Heritabilities ranged from 0.00-0.88. These results suggest that because heritabilities of BW, TL, SL, BH, CTH and HL for sires in Oreochromis niloticus for 180 and 210 days old are low, family selection or progeny testing can proposed for selection. But, therefore heritabilities of all traits both male and female for 300 days old are high, selection will propose for are mass selection.
This study aims to determine the factors affecting von Bertalanffy growth factors and to demonstr... more This study aims to determine the factors affecting von Bertalanffy growth factors and to demonstrate the relationships between these factors. Accordingly, 65 sets of growth data belonging to 43 studies on the subject of the growth of four Mullidae species prevalent in the Mediterranean (Mullus barbatus, Mullus surmuletus, Upeneus pori and Upeneus moluccensis) and the Black Sea (M. barbatus, M. surmuletus). It was discovered that the growth parameters, theoretically affected by similar factors, are not affected by every factor at the same time. It was also discovered that the sample structure given in the studies also affects the biological validity of the parameter estimations.
The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of th... more The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in two tilapia species, Oreochromis aureus and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435 and 342 bp, respectively) were amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate restriction endonuclease enzymes and analysed using agarose gel electrophoresis.
β-Glucan is an essential component of the cell walls of grains such as oats and barley. 1,3-1,4-β... more β-Glucan is an essential component of the cell walls of grains such as oats and barley. 1,3-1,4-β-D-glucan 4-glucanhydrolase (β-glucanase or lichenase) (EC 3.2.1.73) is an enzyme with the ability to hydrolyze β-glucans. In this research, β-Glucan which is a good source of feed additive fi sh probiotics, was used in order to benefi t from feed quality in fi shery products, to increase live weight gain and to strengthen the immune system. In this study, recombinant vector pNW33N carrying the β-(1,3-1,4) glucanase (lichenase) gene of Streptococcus bovis genome was transferred to Bacillus subtilis RSKK246 (CMCase+) strain by electroporation. Subsequently, electrotransformation was performed on LB-agar plates containing lichenan and enzymatic activity regions of recombinant B. subtilis RSKK246 colonies were observed by staining with Congo red. In addition, the DNA from the recombinant plasmid pNW33N+Lichenase (pNW33NLic) was cut on both the BamHI and HindIII endonucleases and observed on the lichenase gene (1800 bp) agarose gel. On the other hand, the protein band corresponding to 26 kDa of the recombinant enzyme was observed by zymogram analysis. These results indicate that the β-(1,3-1,4) glucanase gene has been successfully expressed to the B. subtilis strain RSKK246.
Giriş: Silaj, yeşil yem bitkilerinin anaerobik koşullar altında fermente edilerek saklanmasıyla e... more Giriş: Silaj, yeşil yem bitkilerinin anaerobik koşullar altında fermente edilerek saklanmasıyla elde edilen, besin madde bakımından oldukça zengin ve tercih edilebilir bir kaba yem kaynağıdır. Silaj açıldıktan sonra hava ile teması sonucu, silaj aerobik küf ve maya kontaminasyonuna maruz kalmakta ve sonuçta ciddi ekonomik kayıplar ve sağlık açısından riskler ortaya çıkmaktadır. Bu çalışmada Cellulosimicrobium cellulans’a ait β-1,3-glukanaz geninin Lactobacillus plantarum’da klonlanması ile aerobik bozulmayı önleyen rekombinant silaj inokülantının geliştirilmesi amaçlanmıştır. Gereçler ve Yöntem: Bu çalışmada β-1,3-glukanaz gen kaynağı olarak C. cellulans bakterisi kullanılmıştır. Gen PCR yöntemi ile bakterinin genomundan amplifiye edilmiştir. Klonlama vektörleri olarak pUC18 ve pTE353-2 plazmit DNA’ları kullanılmıştır. Geni taşıyan rekombinant plazmit DNA’ların Escherichia coli ve L. plantarum’a aktarılmasında sırasıyla CaCl2 transformasyonu ve elektroporasyon teknikleri kullanılmış...
Lysozyme (EC.3.2.1.17) is an enzyme that catalyses the hydrolysis of beta-1,4 glucosidic linkages... more Lysozyme (EC.3.2.1.17) is an enzyme that catalyses the hydrolysis of beta-1,4 glucosidic linkages between N-acetyl glucosamine (NAG) and N-acetyl muramic acid (NAM) in the peptidoglucan heteropolymers of prokaryotic cell walls. In this study, lysozyme enzyme found in chicken egg white was treated under different pH conditions (pH 4.0, 6.0, 8.0, 10.0, 12.0) and the results were observed as electrophoretic. Electrophoretic results revealed that egg white lysozyme was best yielded at pH 4.0. As a result of dropping the egg white solution on the Bacillus subtilis spreaded on the LB-agar plates with a glass rod, it was observed that the bacteria could not developed due to the lysozyme of egg white. On the other hand, genomic and plasmid DNAs were isolated from the bacteria in DNA isolation studies in which the egg white solution was replaced by commercial lysozyme. The results showed that, in the absence of commercial lysozyme under laboratory conditions, chicken egg white can be used as...
Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi
To obtain high efficiency from animals, it is necessary to maximize the ability to benefit from f... more To obtain high efficiency from animals, it is necessary to maximize the ability to benefit from feed, as well as to protect animal health. One of the important applications in this direction is feed additives. Feed additives are plant and animal products and microorganisms that increase fish production and welfare, improve digestion and digestive system microflora and contribute to the preservation of nutrients and feed. Although plant and animal tissues are used in enzyme production, the use of microorganisms is in the first place. After the use of molecular genetic techniques, the genes responsible for enzyme production were cloned in microorganisms, making it possible to produce enzymes more purely and cheaply on an industrial scale.
Bu çalışmayla, tütsülenmiş ve marine edilmiş gökkuşağı alabalığının (Oncorhynchus mykiss) buzdola... more Bu çalışmayla, tütsülenmiş ve marine edilmiş gökkuşağı alabalığının (Oncorhynchus mykiss) buzdolabında depolama süresince elektroforetik protein proflindeki değişimlerin belirlenmesi amaçlanmıştır. Çiğ, tütsülenmiş ve marine edilmiş alabalık örneklerinin , 9 haftalık buzdolabında depolama süresince kas proteinlerindeki değişimler sodyum dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE) kullanılarak incelenmiştir. SDS-PAGE ve densitometrik analiz sonuçlarına göre, tütsülenmiş ve marine edilmiş alabalık kaslarının, aktin bandlarında önemli bir değişimin olmadığı, buna karşılık bazı protein banlarının sayısında ve yoğunluğunda bir azalmanın meydana geldiği gözlemlenmiştir. Depolamanın 9. haftasında, 205 kDa molekül ağırlığındaki myosin ağır zinciri (MHC), gözden kaybolmuştur. Ayrıca tütsülenmiş ve marine edilmiş örneklerin protein yüzdeleri de haftalık olarak önemli oranda değişim göstermiştir (P<0.01). Çiğ, tütsülenmiş ve marine edilmiş örneklerin protein yüzdeleri sırasıyl...
In this study, serin esterase gene from Spirulina platensis amplified by
PCR using primer sequen... more In this study, serin esterase gene from Spirulina platensis amplified by
PCR using primer sequences. The amplified gene cloned into the pBR325 vector,
thus, entegrative vectors for S. platensis were constructed. The new entegrative
vector were identified as pBR325SE2. Bacteriophage T4 lysosyme gene was
cloned into pBR325SE2 and the new vector harbouring lysozyme gene was
identified as pBR325SL. pBR325SL was transferred into competent E. coli and S.
platensis respectively. Recombinant S. platensis/pBR325SL grown in the Spirulina
medium with cml while there was no non-transformant Spirulina in the same
medium but intensive non-transformant growth was observed in the medium
without cml. While the presence of lysozyme gene was demonstrated in pBR325SL
isolated from E. coli by PCR, but it couldn’t be demonstrated in S.
platensis/pBR325SL because of insufficient Spirulina in the medium with cml
growth for DNA isolation
TEZ6224Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2007.Kaynakça (s.72-76) var.xiii, 84 s. : r... more TEZ6224Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2007.Kaynakça (s.72-76) var.xiii, 84 s. : rnk. ; 29 cm.In this study, serin esterase gene from Spirulina platensis amplified by PCR using two different primer sequences. The amplified gene cloned into the pBR325 vector in two different way, depending on differences between primers, thus, two entegrative vectors for S. platensis were constructed. The new entegrative vectors were identified as pBR325SE1 and pBR325SE2 respectively. While pBR325SE1 and pBR325SE2 were transferred into competent E. coli, besides this, pBR325SE1 was electroporated into S. platensis. Recombinant S. platensis/pBR325SE1 were grown in the Spirulina medium with chloramphenicol (cml) while there wasn't any non-transformant Spirulina in the same medium but intensive non-transformant growth was observed in the medium without cml. Bacteriophage T4 lysosyme gene was cloned into pBR325SE2 and the new vector harbouring lysozyme gene was identified as pBR325SL. ...
The aim of this study was to compare Bayesian and Classical estimation for describing the growth ... more The aim of this study was to compare Bayesian and Classical estimation for describing the growth curves of red mullet (Mullus barbatus barbatus Linnaeus, 1758). Classical nonlinear regression method and Bayesian estimation method were used to obtain the estimation of the components of the von Bertalanffy growth model. The estimated parameters showed that Bayesian estimation is better than Classical nonlinear regression in estimating growth parameters and reducing variation of growth model parameters.
Objective: This study presents 660 length-weight relationships gathered from literature related t... more Objective: This study presents 660 length-weight relationships gathered from literature related to 225 fish species, throughout Turkish marine waters. Methods: All length-weight relationships presented here are the product of published 27 length-weight relationship studies conducted during 1997-2011 in Turkish marine waters. Summary of length-weight relationship parameters (a and b), was presented and both of regression parameters was compared with genus, season and study area using analysis of variance (ANOVA) and analysis covariance (ANCOVA). Correlation between parameters a and b was calculated across species. Results and Discussion: Overall, 13 out of the 660 length-weight relationships refer to Mullus barbatus (2%), 12 (1.8%) to Diplodus annularis and 12 (1.8%) to Arnoglossus laterna. In terms2.9%) relationships, respectively. Most r 2 values being greater than 0.80 (28 of 660 relationships were smaller than 0.80). Across species mean of b (3.016) is not significantly larger th...
Abstract The aim of this study was to determine the effects of different cooking methods on the e... more Abstract The aim of this study was to determine the effects of different cooking methods on the electrophoretic patterns of rainbow trout (Oncorhynchus mykiss) fillets using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Raw rainbow trout were ...
In order to put cultured species on the market with high quality and few casualties, many importa... more In order to put cultured species on the market with high quality and few casualties, many important studies are carried out. Most of the researches are conducted in the development of feed and feed ingredients 30-60% of the production cost in farming. Therefore, in aquaculture, an interest in alternative feed ingredients is moving at a very fast rate. In this context, the use of enzymes, probiotics and prebiotics in animal feed has steadily increased in recent years with reasons such as effective control of fish diseases and prevention of infection, strengthening the immune system of fish, increase of the digestibility, reduction of the feed cost, reduction of larval-term mortality, provision of increase in growth, live weight gain, and getting rid of the negative effects of stress.
DESCRIPTION Türkiye’nin Akdeniz kıyılarında yayılış gösteren 26 lesepsiyen türe ait yayınlanmış 1... more DESCRIPTION Türkiye’nin Akdeniz kıyılarında yayılış gösteren 26 lesepsiyen türe ait yayınlanmış 11 çalışmadan toplam 63 boy ağırlık ilişkisi Google Scholar, Academia edu, Researchgate ve Scopus gibi veri tabanı/arama motorlarından elde edilmiş ve incelenmiştir.
The aim of this study is to compare Bayesian and Classical estimation for describing the growth c... more The aim of this study is to compare Bayesian and Classical estimation for describing the growth curves of Brushtooth Lizardfish (Saurida lessepsianus Russell, Golani & Tikochinski 2015). Classical nonlinear regression method and Bayesian estimation method were used to obtain the estimation of the components of the von Bertalanffy growth model. The estimated parameters of the von Bertalanffy equation via two methods showed that Bayesian estimation is much better than Classical nonlinear regression in estimating growth parameters and reducing variation of growth model parameters.
Spirulinaisphotosynthetic,�filamentous,� spiral-shaped,�multicellularandgreen-bluemicroalga.�Thet... more Spirulinaisphotosynthetic,�filamentous,� spiral-shaped,�multicellularandgreen-bluemicroalga.�Thetwomostimportantspeciesofwhichare� Spirulina maxima and Spirulina platensis. Since its chemical composition includes proteins, carbohydrates, vitamins, minerals, essential fatty acids andpigmentslikechlorophylla, carotenes and phycocyanin, it is considered an excellent food and feed. It also used in medicine and cosmetic industries.�Becauseofitsnutritional,�medicalandbiologicalvalue,�geneticstudiesonSpirulinahavebeenincreasedallovertheworldtodevelop� newstrainsgainednewproperties.
The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of th... more The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in two tilapia species, Oreochromis aureus and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435 and 342 bp, respectively) were amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate restriction endonuclease enzymes and analysed using agarose gel electrophoresis. Link of the (Abstract) Paper: https://www.wflpublisher.com/Abstract/3998 Link of the website: https://www.wflpublisher.com
This study aimed to determine the effects on protein of frozen storage time (-20 °C), and on prop... more This study aimed to determine the effects on protein of frozen storage time (-20 °C), and on properties of raw, mar-inated and smoked rainbow trouts (Oncorhynchus mykiss). Therefore, the fish were smoked and marinated. All filleted fish samples including also the raw ones were vacuum-packed, subjected to 9 months of frozen storage and analyzed at 1-month intervals. Changes in muscle proteins of raw, smoked and marinated trout samples (Oncorhynchus mykiss) have been examined using sodium dodecyl sul-phate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE and densitometric analysis revealed that intensity and the number of protein bands were not changed until the 9 th month of raw and marinating process while the bands of some protein patterns denaturated when trout muscle was subjected to 9 months of smoking process. My-osin heavy chain (MHC) band with molecular weights of 205 kDa and actin band with molecular weights of 45 kDa appeared during 9 months of raw, smoking and marinating process. Similarly, other main myofibrillar proteins, tropo-myosin (37.7 kDa), troponin-T (32.9 kDa), myosin light chain 1 (27.2 kDa), and myosin light chain 2 (20.4 kDa) also did not show any differences during 9-months storage. Subsequently, there were significant monthly changes in the protein percentages caused by smoking and marinating processes excluding the raw ones (P&lt;0.01).
Heritability estimates were determined for six phenotypes in Oreochromis niloticus for 180, 210 a... more Heritability estimates were determined for six phenotypes in Oreochromis niloticus for 180, 210 and 300 days old: body weight (BW), total length (TL), standard length (SL), body height (BH), caudal tail height (CTH) and head length (HL). Heritability estimates were calculated by half-sib analysis from an experiment that contained 3 paternal half-sib families and 11 full-sib families. Heritabilities ranged from 0.00-0.88. These results suggest that because heritabilities of BW, TL, SL, BH, CTH and HL for sires in Oreochromis niloticus for 180 and 210 days old are low, family selection or progeny testing can proposed for selection. But, therefore heritabilities of all traits both male and female for 300 days old are high, selection will propose for are mass selection.
This study aims to determine the factors affecting von Bertalanffy growth factors and to demonstr... more This study aims to determine the factors affecting von Bertalanffy growth factors and to demonstrate the relationships between these factors. Accordingly, 65 sets of growth data belonging to 43 studies on the subject of the growth of four Mullidae species prevalent in the Mediterranean (Mullus barbatus, Mullus surmuletus, Upeneus pori and Upeneus moluccensis) and the Black Sea (M. barbatus, M. surmuletus). It was discovered that the growth parameters, theoretically affected by similar factors, are not affected by every factor at the same time. It was also discovered that the sample structure given in the studies also affects the biological validity of the parameter estimations.
The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of th... more The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in two tilapia species, Oreochromis aureus and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435 and 342 bp, respectively) were amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate restriction endonuclease enzymes and analysed using agarose gel electrophoresis.
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Papers by Makbule Baylan
PCR using primer sequences. The amplified gene cloned into the pBR325 vector,
thus, entegrative vectors for S. platensis were constructed. The new entegrative
vector were identified as pBR325SE2. Bacteriophage T4 lysosyme gene was
cloned into pBR325SE2 and the new vector harbouring lysozyme gene was
identified as pBR325SL. pBR325SL was transferred into competent E. coli and S.
platensis respectively. Recombinant S. platensis/pBR325SL grown in the Spirulina
medium with cml while there was no non-transformant Spirulina in the same
medium but intensive non-transformant growth was observed in the medium
without cml. While the presence of lysozyme gene was demonstrated in pBR325SL
isolated from E. coli by PCR, but it couldn’t be demonstrated in S.
platensis/pBR325SL because of insufficient Spirulina in the medium with cml
growth for DNA isolation
PCR using primer sequences. The amplified gene cloned into the pBR325 vector,
thus, entegrative vectors for S. platensis were constructed. The new entegrative
vector were identified as pBR325SE2. Bacteriophage T4 lysosyme gene was
cloned into pBR325SE2 and the new vector harbouring lysozyme gene was
identified as pBR325SL. pBR325SL was transferred into competent E. coli and S.
platensis respectively. Recombinant S. platensis/pBR325SL grown in the Spirulina
medium with cml while there was no non-transformant Spirulina in the same
medium but intensive non-transformant growth was observed in the medium
without cml. While the presence of lysozyme gene was demonstrated in pBR325SL
isolated from E. coli by PCR, but it couldn’t be demonstrated in S.
platensis/pBR325SL because of insufficient Spirulina in the medium with cml
growth for DNA isolation