primer3_core
tool to design flanking oligo nucleotides for DNA amplification
Install
- All systems
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curl cmd.cat/primer3_core.sh
- Debian
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apt-get install primer3 - Ubuntu
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apt-get install primer3 - Kali Linux
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apt-get install primer3 - Fedora
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dnf install primer3 - Windows (WSL2)
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sudo apt-get updatesudo apt-get install primer3 - OS X
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brew install primer3 - Raspbian
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apt-get install primer3
primer3
tool to design flanking oligo nucleotides for DNA amplification
Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.