James Hutchins

Centre National de la Recherche Scientifique / French National Centre for Scientific Research, Institute of Human Genetics (IGH), Researcher

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Researcher investigating proteins controlling chromosome duplication and cell division.

Previous postdocs:

2004-2010
Institute of Molecular Pathology (IMP), Vienna
Lab of Jan-Michael Peters

2000-2004
Biomedical Research Centre
University of Dundee
Lab of Paul R. Clarke

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James Elkins

School of the Art Institute of Chicago

Asha Vijayan

Amrita Vishwa Vidyapeetham

Dr.Mahmoud Lotfy

Minufiya University

Rebecca Riggins

Georgetown University

Gwen Robbins Schug

University of North Carolina at Greensboro

William R. Buckley

Paul R Jaschke

Macquarie University

Hanaa Ahmed

Animal Health Research Institute

Richard Cloutier

Université du Québec à Rimouski

Irina Kolesnik

Moscow State University

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Papers by James Hutchins

Biomarkers for polo-like kinase 1 activity

by James Hutchins, O. Hudecz, Björn Hegemann, and Karl Mechtler

Proteomic data on the nuclear interactome of human MCM9

Data in Brief, 2016

We present data relating to the interactome of MCM9 from the nuclei of human cells. MCM9 belongs ... more We present data relating to the interactome of MCM9 from the nuclei of human cells. MCM9 belongs to the AAA+ superfamily, and contains an MCM domain and motifs that may confer DNA helicase activity. MCM9 has been shown to bind MCM8, and has been implicated in DNA replication and homologous recombination. However, the mechanistic basis of MCM9&amp;amp;amp;amp;#39;s role in DNA repair is poorly understood, and proteins with which it interacts were hitherto unknown. We performed tandem affinity purification of MCM9 and its interacting proteins from nuclear extracts of human cells, followed by proteomic analysis, thereby generating a set of mass spectrometry data corresponding to the MCM9 interactome [1]. The proteomic data set comprises 29 mass spectrometry RAW files, deposited to the ProteomeXchange Consortium, and freely available from the PRIDE partner repository with the data set identifier PXD000212. A set of 22 interacting proteins identified from the proteomic data was used to create an MCM9-centered interactive network diagram, using the Cytoscape program. These data allow the scientific community to access, mine and explore the human nuclear MCM9 interactome.

Extracting order from heterogeneity: A report on the EpiGeneSys workshop "Single Cell Epigenetics" in Montpellier, June 11-12, 2015

BioEssays : news and reviews in molecular, cellular and developmental biology, Jan 16, 2015

Understanding epigenetic modifications to chromatin that regulate gene expression and cell-fate d... more Understanding epigenetic modifications to chromatin that regulate gene expression and cell-fate decisions is now possible in single cells thanks to recent technological advances. As interdisciplinary approaches are required to derive biological principles, this workshop brought together some of Europe's leading researchers in single-cell epigenetics to share technologies and biological insights.

MCM9 Is Required for Mammalian DNA Mismatch Repair

Molecular Cell, 2015

DNA mismatch repair (MMR) is an evolutionarily conserved process that corrects DNA polymerase err... more DNA mismatch repair (MMR) is an evolutionarily conserved process that corrects DNA polymerase errors during replication to maintain genomic integrity. In E. coli, the DNA helicase UvrD is implicated in MMR, yet an analogous helicase activity has not been identified in eukaryotes. Here, we show that mammalian MCM9, a protein involved in replication and homologous recombination, forms a complex with MMR initiation proteins (MSH2, MSH3, MLH1, PMS1, and the clamp loader RFC) and is essential for MMR. Mcm9-/- cells display microsatellite instability and MMR deficiency. The MCM9 complex has a helicase activity that is required for efficient MMR since wild-type but not helicase-dead MCM9 restores MMR activity in Mcm9-/- cells. Moreover, MCM9 loading onto chromatin is MSH2-dependent, and in turn MCM9 stimulates the recruitment of MLH1 to chromatin. Our results reveal a role for MCM9 and its helicase activity in mammalian MMR.

What’s that gene (or protein)? Online resources for exploring functions of genes, transcripts, and proteins

The genomic era has enabled research projects that use approaches including genome-scale screens,... more The genomic era has enabled research projects that use approaches including genome-scale screens, microarray analysis, next-generation sequencing, and mass spectrometry-based proteomics to discover genes and proteins involved in biological processes. Such methods generate data sets of gene, transcript, or protein hits that researchers wish to explore to understand their properties and functions and thus their possible roles in biological systems of interest. Recent years have seen a profusion of Internet-based resources to aid this process. This review takes the viewpoint of the curious biologist wishing to explore the properties of protein-coding genes and their products, identified using genome-based technologies. Ten key questions are asked about each hit, addressing functions, phenotypes, expression, evolutionary conservation, disease association, protein structure, interactors, posttranslational modifications, and inhibitors. Answers are provided by presenting the latest publicly available resources, together with methods for hit-specific and data set-wide information retrieval, suited to any genome-based analytical technique and experimental species.

James Hutchins

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