This study evaluated the effects of the probiotic Bacillus spp. isolated from African nightcrawle... more This study evaluated the effects of the probiotic Bacillus spp. isolated from African nightcrawler (Eudrilus eugeniae) on the growth and survival performances of Nile tilapia (Oreochromis niloticus). A total of 400 Nile tilapia fingerlings (1.76 ± 0.07 g) were equally and randomly distributed into 20 glass aquaria and divided into five experimental groups with four replicates. Each experimental group was fed with a commercial diet (control) supplemented (at 10 8 CFU·g −1) with one of the four Bacillus spp. (ANSCI9, BFAR9, RM3, and RM10) at five percent of their body weight. After 30 days of feeding, the surviving fish were challenged with Aeromonas hydrophila to observe their survival. The treatment groups supplemented with BFAR9 (2.73 ± 0.26 g) and RM10 (3.15 ± 0.30 g) showed significantly higher (P < 0.05) absolute growth than the control (2.20 ± 0.16 g) group. Furthermore, those given with RM10 had better (P < 0.05) specific growth rate (SGR) (1.60 ± 0.10%·day −1) relative growth rate (RGR) (181.39 ± 18.16%) and feed conversion ratio (FCR) (1.99 ± 0.13) compared to the control group (SGR = 1.29 ± 0.07%·day −1 ; RGR = 129.84 ± 9.77%; FCR = 2.60 ± 0.16). The challenge test revealed that all Bacillus spp.-treated groups showed significantly higher (P < 0.05) survival rates (ANSCI9 = 82.5 ± 8.16%; BFAR9 = 80.0 ± 10.00%; RM3 = 77.5 ± 20.82%; RM10 = 85.0 ± 10.00%) than the control group (55.00 ± 19.15%), with the highest relative level of protection recorded for RM10 (66.67%). These results revealed that the probiotic Bacillus spp. isolated from E. eugeniae improved the growth, feed utilization, waste excretion, and the disease resistance of Nile tilapia.
Journal of Experimental Biology and Agricultural Sciences, Feb 28, 2022
This study was carried out to evaluate the effect of two probiotic Bacillus spp. (RM10 and BFAR9)... more This study was carried out to evaluate the effect of two probiotic Bacillus spp. (RM10 and BFAR9) on the growth, length-weight relationship (LWR), and condition factor (k) of Nile tilapia (Oreochromis niloticus). For this, one hundred thirty-five fingerlings (1.12±0.08 g weight and 1.26±0.15 cm length) were divided into three groups (Control, RM10, and BFAR9) and distributed into nine circular concrete tanks. The fish were fed with commercial (control) and Bacillus spp. supplemented diets at 5% of body weight for 56 days. The results of the study revealed better (P<0.05) growth concerning average body weight (ABW-17.12±0.71g), specific growth rate (SGR-4.89±0.22 g•day-1), absolute growth (AG-16.02±0.78 g), and feed conversion ratio (FCR-1.31±0.09) in the group fed with Bacillus sp. RM10 as compared to the control (ABW-13.25±2.34g; SGR-4.41±0.17g•day-1 ; AG-12.13±2.25g; FCR-1.62±0.11). The LWR in all experimental treatments showed a significant correlation (P<0.05) with an R 2 value of 0.988, 0.966, and 0.979 for Control, RM10, and BFAR9, respectively. The k value revealed that all treatments are in good condition as k value is greater than 1 (1.913, 2.038, and 1.896 for control, RM10, and BFAR9 respectively). The result of the current study revealed that application of Bacillus sp. RM10 improves the growth and feed utilization in Nile tilapia.
Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ... more Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ideal and favorable site for bacterial growth and activity. Hence, in our present study, we isolated and screened potential probiotic bacteria from African nightcrawler (Eudrilus eugeniae). Among 45 bacterial isolates, four (ANSCI9, BFAR9, RM3, and RM10) were selected based on their hydrophobicity, hydrolytic enzyme production, pH and fsh bile tolerance, aggregation, and antimicrobial properties. The selected isolates showed good hydrophobicity (≥30%) and enzyme production (≥10 mm clearing zones), tolerance to pH and fsh bile, and inhibitory properties against pathogenic microorganisms. The isolates were identifed as Bacillus sp. RM3 (MH919306), Bacillus sp. RM10 (MH919308), Bacillus sp. ANSCI9 (MH919310) and Bacillus sp. BFAR9 (MH919302). These isolates were individually incorporated in the diets of Nile tilapia (Oreochromis niloticus) fngerlings for 14 days to assess their biosafety. The results showed that the survival rates in all treated groups (98.75±2.5 to 100.00±0.0%) were not signifcantly diferent (P&lt;0.05) from the control group (commercial diet) (96.25±2.5%), suggesting that isolates have no adverse efect on the host. This study revealed the presence of potential probiotic microorganisms in E. eugeniae that are benefcial to the aquaculture industry.
Journal of Advanced Veterinary and Animal Research, 2020
ABSTRACT Objective: Acute hepatopancreatic necrosis disease is an emerging infectious disease of ... more ABSTRACT Objective: Acute hepatopancreatic necrosis disease is an emerging infectious disease of Penaeus species. The causative agent is Vibrio species, which dispels binary toxin similar to pirA and pirB, which causes mortality in infected shrimp. The aim of this research was to investigate the evolutionary relationship of pirA and pirB homologous genes present in this Asia and Costa Rica in silico. Materials and methods: The sequences for in silico analysis were all retrieved from the Basic Local Alignment Search Tool Nucleotide (BLASTN) tool of the National Center for Biotechnology Center. For pirA, a total of 25 sequences submitted from different Asian countries and Costa Rica were retrieved for analysis. Meanwhile, for pirB, a total of 11 sequences submitted from five Asian countries were retrieved. Sequences were aligned using the CLUSTAL W alignment tool under Molecular Evolutionary Genetics Analysis (MEGA) 7 software. The evolutionary history was then estimated using the Unweighted Pair Group Method with Arithmetic mean (UPGMA) method, whereas the evolutionary distances were determined using the maximum composite likelihood model with 1,000 bootstrap replications. Results and Discussion: The results show that, among 27 DNA sequences analyzed for pirA gene, three groups were generated, while for pirB, 13 DNA sequences yielded only one group. The analysis revealed low genetic variation among isolates for both pirA and pirB genes. Conclusion: This result suggests that the low frequency of polymorphism and geographic location cannot be attributed to the differences in V. parahaemolyticus isolates in Asian countries relative to Costa Rican isolates in pirA and pirB genes. ARTICLE HISTORY
World Journal of Microbiology and Biotechnology, Jan 2, 2020
Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ... more Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ideal and favorable site for bacterial growth and activity. Hence, in our present study, we isolated and screened potential probiotic bacteria from African nightcrawler (Eudrilus eugeniae). Among 45 bacterial isolates, four (ANSCI9, BFAR9, RM3, and RM10) were selected based on their hydrophobicity, hydrolytic enzyme production, pH and fsh bile tolerance, aggregation, and antimicrobial properties. The selected isolates showed good hydrophobicity (≥30%) and enzyme production (≥10 mm clearing zones), tolerance to pH and fsh bile, and inhibitory properties against pathogenic microorganisms. The isolates were identifed as Bacillus sp. RM3 (MH919306), Bacillus sp. RM10 (MH919308), Bacillus sp. ANSCI9 (MH919310) and Bacillus sp. BFAR9 (MH919302). These isolates were individually incorporated in the diets of Nile tilapia (Oreochromis niloticus) fngerlings for 14 days to assess their biosafety. The results showed that the survival rates in all treated groups (98.75±2.5 to 100.00±0.0%) were not signifcantly diferent (P<0.05) from the control group (commercial diet) (96.25±2.5%), suggesting that isolates have no adverse efect on the host. This study revealed the presence of potential probiotic microorganisms in E. eugeniae that are benefcial to the aquaculture industry.
International Journal of Fisheries and Aquatic Studies, 2019
The study was conducted in order to provide preliminary information on the physico-chemical, biol... more The study was conducted in order to provide preliminary information on the physico-chemical, biological and bacteriological status of Nabao Lake in Cabiao, Nueva Ecija Philippines. The lake was categorized as hypereutrophic based upon low Secchi disc visibility reading (< 100 cm), low level of DO (<5 ppm) and high level of phosphorus (>0.1 ppm). Identified algal genera in the lake such as Ankistrodesmus, Chlorella, Closterium, Synedra, Euglena and Phacus were considered to be indicators of organic pollution. The water and soil of the lake had a very high total bacterial count (TBB) and total coliform count (TCC).
International Journal of Agricultural Technology, 2019
The present study evaluated the effect of varying levels of garlic (Allium sativum) inclusion on ... more The present study evaluated the effect of varying levels of garlic (Allium sativum) inclusion on the growth, feed utilization and survival of red tilapia (Oreochromis sp.). One hundred twenty red tilapias with initial average body weight of 7.36 ± 0.75 g were randomly introduced into 12 aquaria in 4 groups with 10 fish per aquarium. Treatments were fed with experimental diets for six weeks. The various levels of garlic powder (0, 1%, 1.5% and 2%) was added to commercial diet. At the end of the experiment, growth, survival rates, and feed utilization were evaluated. There was no significant difference in the growth of the fish. The highest survival rate was recorded from 1.5% garlic supplementation that showed significant difference compared to the control diet (P < 0.05). In terms of feed utilization, lowest feed conversion ratio (FCR) was recorded from 1% garlic inclusion that showed significant difference compared to the control diet (P < 0.05). This study showed that addition of 1% to 1.5% garlic powder in the diet improved the feed utilization and survival rate of red tilapia.
A 35 day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal PWC... more A 35 day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal PWC on the growth, feed utilization, survival and waste excretion of red tilapia Oreochromis sp. . Ninety red tilapias with an initial weight of 7.32 ± 1.31 g were acclimatized and randomly introduced into 9 aquaria in 3 groups with 10 fish per aquarium. Different levels of PWC 0 T0 , 1 T1 , and 2 T2 were incorporated in the diets of red tilapia for four weeks. After the experiment, average body weight ABW , specific growth rate SGR , relative growth rate RGR weight gain WG , feed conversion ratio FCR and survival rate SR of the experimental fish were computed. No significant differences were observed on feed utilization, survival, and waste excretion of the fish. Furthermore, ABW and WG of the experimental fish did not show any significant difference, however, significantly higher P 0.05 SGR and RGR were observed from T1 compared to the other treatments. Consequently, this study showed that supplementation of 1 PWC in the diet is the most suitable in improving the growth performance of red tilapia. Jaypee. S. Samson ""Oral Administration of Pulverized Wood Charcoal on Growth, Feed Utilization, Survival and Waste Excretion of Red Tilapia (Oreochromis Sp)"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23724.pdf
International Journal of Trend in Scientific Research and Development, 2019
A 35-day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal (PW... more A 35-day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal (PWC) on the growth, feed utilization, survival and waste excretion of red tilapia (Oreochromis sp.). Ninety red tilapias with an initial weight of 7.32 ± 1.31 g were acclimatized and randomly introduced into 9 aquaria in 3 groups with 10 fish per aquarium. Different levels of PWC (0 (T0), 1% (T1), and 2% (T2)) were incorporated in the diets of red tilapia for four weeks. After the experiment, average body weight (ABW), specific growth rate (SGR), relative growth rate (RGR) weight gain (WG), feed conversion ratio (FCR) and survival rate (SR) of the experimental fish were computed. No significant differences were observed on feed utilization, survival, and waste excretion of the fish. Furthermore, ABW and WG of the experimental fish did not show any significant difference, however, significantly higher (P < 0.05) SGR and RGR were observed from T1 compared to the other treatments. Consequently, this study showed that supplementation of 1% PWC in the diet is the most suitable in improving the growth performance of red tilapia.
Due to the emergence of antibiotic-resistant pathogens, probiotics in aquaculture are used for th... more Due to the emergence of antibiotic-resistant pathogens, probiotics in aquaculture are used for the prevention of infectious microbial diseases and substitute for antibiotics and chemotherapeutics. In this study, we evaluated the effect of probiotic Bacillus spp. isolated from African nightcrawler (Eudrilus eugeniae) on the growth, feed utilization, and disease resistance of Nile tilapia (Oreochromis niloticus). Four probiotic strains of Bacillus spp. (ANSCI9, BFAR9, RM3, and RM10) were individually incorporated in the commercial diet (control) at 108 CFU g-1of feed. The experimental fish were fed at 5% of their body weight for 30 days, and subjected to a 14-day Aeromonas hydrophila challenge test afterward. The results showed the probiotic-treated groups have higher (P<0.05) average body weight (ABW) (4.51 ± 0.34 g) than the control (3.89 ± 0.17 g). The BFAR9 (2.73 ± 0.26 g) and RM10 (3.15 ± 0.30 g) showed higher (P<0.05) absolute growth (AG) than the control (2.20 ± 0.16 g). Furthermore, RM10 had higher (P<0.05) specific growth rate (SGR) (1.60 ± 0.10 % day-1) and relative growth rate (RGR) (181.39 ± 18.16 %) than the control (SGR=1.29 ± 0.07 % day-1 ; RGR=129.84 ± 9.77 %). Consequently, RM10 had significantly lower (P<0.05) feed conversion ratio (FCR) (1.99 ± 0.13) than the control (2.60 ± 0.16). The challenge test revealed that the probiotic-treated groups have higher (P<0.05) survival (81.25 ± 9.57 %) than the control (55.00 ± 19.15 %). These results revealed that the probiotic Bacillus spp. isolated from E. eugeniae improved the growth, feed utilization, and the disease resistance of Nile tilapia.
This study evaluated the effects of the probiotic Bacillus spp. isolated from African nightcrawle... more This study evaluated the effects of the probiotic Bacillus spp. isolated from African nightcrawler (Eudrilus eugeniae) on the growth and survival performances of Nile tilapia (Oreochromis niloticus). A total of 400 Nile tilapia fingerlings (1.76 ± 0.07 g) were equally and randomly distributed into 20 glass aquaria and divided into five experimental groups with four replicates. Each experimental group was fed with a commercial diet (control) supplemented (at 10 8 CFU·g −1) with one of the four Bacillus spp. (ANSCI9, BFAR9, RM3, and RM10) at five percent of their body weight. After 30 days of feeding, the surviving fish were challenged with Aeromonas hydrophila to observe their survival. The treatment groups supplemented with BFAR9 (2.73 ± 0.26 g) and RM10 (3.15 ± 0.30 g) showed significantly higher (P < 0.05) absolute growth than the control (2.20 ± 0.16 g) group. Furthermore, those given with RM10 had better (P < 0.05) specific growth rate (SGR) (1.60 ± 0.10%·day −1) relative growth rate (RGR) (181.39 ± 18.16%) and feed conversion ratio (FCR) (1.99 ± 0.13) compared to the control group (SGR = 1.29 ± 0.07%·day −1 ; RGR = 129.84 ± 9.77%; FCR = 2.60 ± 0.16). The challenge test revealed that all Bacillus spp.-treated groups showed significantly higher (P < 0.05) survival rates (ANSCI9 = 82.5 ± 8.16%; BFAR9 = 80.0 ± 10.00%; RM3 = 77.5 ± 20.82%; RM10 = 85.0 ± 10.00%) than the control group (55.00 ± 19.15%), with the highest relative level of protection recorded for RM10 (66.67%). These results revealed that the probiotic Bacillus spp. isolated from E. eugeniae improved the growth, feed utilization, waste excretion, and the disease resistance of Nile tilapia.
Journal of Experimental Biology and Agricultural Sciences, Feb 28, 2022
This study was carried out to evaluate the effect of two probiotic Bacillus spp. (RM10 and BFAR9)... more This study was carried out to evaluate the effect of two probiotic Bacillus spp. (RM10 and BFAR9) on the growth, length-weight relationship (LWR), and condition factor (k) of Nile tilapia (Oreochromis niloticus). For this, one hundred thirty-five fingerlings (1.12±0.08 g weight and 1.26±0.15 cm length) were divided into three groups (Control, RM10, and BFAR9) and distributed into nine circular concrete tanks. The fish were fed with commercial (control) and Bacillus spp. supplemented diets at 5% of body weight for 56 days. The results of the study revealed better (P<0.05) growth concerning average body weight (ABW-17.12±0.71g), specific growth rate (SGR-4.89±0.22 g•day-1), absolute growth (AG-16.02±0.78 g), and feed conversion ratio (FCR-1.31±0.09) in the group fed with Bacillus sp. RM10 as compared to the control (ABW-13.25±2.34g; SGR-4.41±0.17g•day-1 ; AG-12.13±2.25g; FCR-1.62±0.11). The LWR in all experimental treatments showed a significant correlation (P<0.05) with an R 2 value of 0.988, 0.966, and 0.979 for Control, RM10, and BFAR9, respectively. The k value revealed that all treatments are in good condition as k value is greater than 1 (1.913, 2.038, and 1.896 for control, RM10, and BFAR9 respectively). The result of the current study revealed that application of Bacillus sp. RM10 improves the growth and feed utilization in Nile tilapia.
Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ... more Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ideal and favorable site for bacterial growth and activity. Hence, in our present study, we isolated and screened potential probiotic bacteria from African nightcrawler (Eudrilus eugeniae). Among 45 bacterial isolates, four (ANSCI9, BFAR9, RM3, and RM10) were selected based on their hydrophobicity, hydrolytic enzyme production, pH and fsh bile tolerance, aggregation, and antimicrobial properties. The selected isolates showed good hydrophobicity (≥30%) and enzyme production (≥10 mm clearing zones), tolerance to pH and fsh bile, and inhibitory properties against pathogenic microorganisms. The isolates were identifed as Bacillus sp. RM3 (MH919306), Bacillus sp. RM10 (MH919308), Bacillus sp. ANSCI9 (MH919310) and Bacillus sp. BFAR9 (MH919302). These isolates were individually incorporated in the diets of Nile tilapia (Oreochromis niloticus) fngerlings for 14 days to assess their biosafety. The results showed that the survival rates in all treated groups (98.75±2.5 to 100.00±0.0%) were not signifcantly diferent (P&lt;0.05) from the control group (commercial diet) (96.25±2.5%), suggesting that isolates have no adverse efect on the host. This study revealed the presence of potential probiotic microorganisms in E. eugeniae that are benefcial to the aquaculture industry.
Journal of Advanced Veterinary and Animal Research, 2020
ABSTRACT Objective: Acute hepatopancreatic necrosis disease is an emerging infectious disease of ... more ABSTRACT Objective: Acute hepatopancreatic necrosis disease is an emerging infectious disease of Penaeus species. The causative agent is Vibrio species, which dispels binary toxin similar to pirA and pirB, which causes mortality in infected shrimp. The aim of this research was to investigate the evolutionary relationship of pirA and pirB homologous genes present in this Asia and Costa Rica in silico. Materials and methods: The sequences for in silico analysis were all retrieved from the Basic Local Alignment Search Tool Nucleotide (BLASTN) tool of the National Center for Biotechnology Center. For pirA, a total of 25 sequences submitted from different Asian countries and Costa Rica were retrieved for analysis. Meanwhile, for pirB, a total of 11 sequences submitted from five Asian countries were retrieved. Sequences were aligned using the CLUSTAL W alignment tool under Molecular Evolutionary Genetics Analysis (MEGA) 7 software. The evolutionary history was then estimated using the Unweighted Pair Group Method with Arithmetic mean (UPGMA) method, whereas the evolutionary distances were determined using the maximum composite likelihood model with 1,000 bootstrap replications. Results and Discussion: The results show that, among 27 DNA sequences analyzed for pirA gene, three groups were generated, while for pirB, 13 DNA sequences yielded only one group. The analysis revealed low genetic variation among isolates for both pirA and pirB genes. Conclusion: This result suggests that the low frequency of polymorphism and geographic location cannot be attributed to the differences in V. parahaemolyticus isolates in Asian countries relative to Costa Rican isolates in pirA and pirB genes. ARTICLE HISTORY
World Journal of Microbiology and Biotechnology, Jan 2, 2020
Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ... more Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ideal and favorable site for bacterial growth and activity. Hence, in our present study, we isolated and screened potential probiotic bacteria from African nightcrawler (Eudrilus eugeniae). Among 45 bacterial isolates, four (ANSCI9, BFAR9, RM3, and RM10) were selected based on their hydrophobicity, hydrolytic enzyme production, pH and fsh bile tolerance, aggregation, and antimicrobial properties. The selected isolates showed good hydrophobicity (≥30%) and enzyme production (≥10 mm clearing zones), tolerance to pH and fsh bile, and inhibitory properties against pathogenic microorganisms. The isolates were identifed as Bacillus sp. RM3 (MH919306), Bacillus sp. RM10 (MH919308), Bacillus sp. ANSCI9 (MH919310) and Bacillus sp. BFAR9 (MH919302). These isolates were individually incorporated in the diets of Nile tilapia (Oreochromis niloticus) fngerlings for 14 days to assess their biosafety. The results showed that the survival rates in all treated groups (98.75±2.5 to 100.00±0.0%) were not signifcantly diferent (P<0.05) from the control group (commercial diet) (96.25±2.5%), suggesting that isolates have no adverse efect on the host. This study revealed the presence of potential probiotic microorganisms in E. eugeniae that are benefcial to the aquaculture industry.
International Journal of Fisheries and Aquatic Studies, 2019
The study was conducted in order to provide preliminary information on the physico-chemical, biol... more The study was conducted in order to provide preliminary information on the physico-chemical, biological and bacteriological status of Nabao Lake in Cabiao, Nueva Ecija Philippines. The lake was categorized as hypereutrophic based upon low Secchi disc visibility reading (< 100 cm), low level of DO (<5 ppm) and high level of phosphorus (>0.1 ppm). Identified algal genera in the lake such as Ankistrodesmus, Chlorella, Closterium, Synedra, Euglena and Phacus were considered to be indicators of organic pollution. The water and soil of the lake had a very high total bacterial count (TBB) and total coliform count (TCC).
International Journal of Agricultural Technology, 2019
The present study evaluated the effect of varying levels of garlic (Allium sativum) inclusion on ... more The present study evaluated the effect of varying levels of garlic (Allium sativum) inclusion on the growth, feed utilization and survival of red tilapia (Oreochromis sp.). One hundred twenty red tilapias with initial average body weight of 7.36 ± 0.75 g were randomly introduced into 12 aquaria in 4 groups with 10 fish per aquarium. Treatments were fed with experimental diets for six weeks. The various levels of garlic powder (0, 1%, 1.5% and 2%) was added to commercial diet. At the end of the experiment, growth, survival rates, and feed utilization were evaluated. There was no significant difference in the growth of the fish. The highest survival rate was recorded from 1.5% garlic supplementation that showed significant difference compared to the control diet (P < 0.05). In terms of feed utilization, lowest feed conversion ratio (FCR) was recorded from 1% garlic inclusion that showed significant difference compared to the control diet (P < 0.05). This study showed that addition of 1% to 1.5% garlic powder in the diet improved the feed utilization and survival rate of red tilapia.
A 35 day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal PWC... more A 35 day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal PWC on the growth, feed utilization, survival and waste excretion of red tilapia Oreochromis sp. . Ninety red tilapias with an initial weight of 7.32 ± 1.31 g were acclimatized and randomly introduced into 9 aquaria in 3 groups with 10 fish per aquarium. Different levels of PWC 0 T0 , 1 T1 , and 2 T2 were incorporated in the diets of red tilapia for four weeks. After the experiment, average body weight ABW , specific growth rate SGR , relative growth rate RGR weight gain WG , feed conversion ratio FCR and survival rate SR of the experimental fish were computed. No significant differences were observed on feed utilization, survival, and waste excretion of the fish. Furthermore, ABW and WG of the experimental fish did not show any significant difference, however, significantly higher P 0.05 SGR and RGR were observed from T1 compared to the other treatments. Consequently, this study showed that supplementation of 1 PWC in the diet is the most suitable in improving the growth performance of red tilapia. Jaypee. S. Samson ""Oral Administration of Pulverized Wood Charcoal on Growth, Feed Utilization, Survival and Waste Excretion of Red Tilapia (Oreochromis Sp)"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23724.pdf
International Journal of Trend in Scientific Research and Development, 2019
A 35-day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal (PW... more A 35-day feeding experiment was conducted to evaluate the effects of pulverized wood charcoal (PWC) on the growth, feed utilization, survival and waste excretion of red tilapia (Oreochromis sp.). Ninety red tilapias with an initial weight of 7.32 ± 1.31 g were acclimatized and randomly introduced into 9 aquaria in 3 groups with 10 fish per aquarium. Different levels of PWC (0 (T0), 1% (T1), and 2% (T2)) were incorporated in the diets of red tilapia for four weeks. After the experiment, average body weight (ABW), specific growth rate (SGR), relative growth rate (RGR) weight gain (WG), feed conversion ratio (FCR) and survival rate (SR) of the experimental fish were computed. No significant differences were observed on feed utilization, survival, and waste excretion of the fish. Furthermore, ABW and WG of the experimental fish did not show any significant difference, however, significantly higher (P < 0.05) SGR and RGR were observed from T1 compared to the other treatments. Consequently, this study showed that supplementation of 1% PWC in the diet is the most suitable in improving the growth performance of red tilapia.
Due to the emergence of antibiotic-resistant pathogens, probiotics in aquaculture are used for th... more Due to the emergence of antibiotic-resistant pathogens, probiotics in aquaculture are used for the prevention of infectious microbial diseases and substitute for antibiotics and chemotherapeutics. In this study, we evaluated the effect of probiotic Bacillus spp. isolated from African nightcrawler (Eudrilus eugeniae) on the growth, feed utilization, and disease resistance of Nile tilapia (Oreochromis niloticus). Four probiotic strains of Bacillus spp. (ANSCI9, BFAR9, RM3, and RM10) were individually incorporated in the commercial diet (control) at 108 CFU g-1of feed. The experimental fish were fed at 5% of their body weight for 30 days, and subjected to a 14-day Aeromonas hydrophila challenge test afterward. The results showed the probiotic-treated groups have higher (P<0.05) average body weight (ABW) (4.51 ± 0.34 g) than the control (3.89 ± 0.17 g). The BFAR9 (2.73 ± 0.26 g) and RM10 (3.15 ± 0.30 g) showed higher (P<0.05) absolute growth (AG) than the control (2.20 ± 0.16 g). Furthermore, RM10 had higher (P<0.05) specific growth rate (SGR) (1.60 ± 0.10 % day-1) and relative growth rate (RGR) (181.39 ± 18.16 %) than the control (SGR=1.29 ± 0.07 % day-1 ; RGR=129.84 ± 9.77 %). Consequently, RM10 had significantly lower (P<0.05) feed conversion ratio (FCR) (1.99 ± 0.13) than the control (2.60 ± 0.16). The challenge test revealed that the probiotic-treated groups have higher (P<0.05) survival (81.25 ± 9.57 %) than the control (55.00 ± 19.15 %). These results revealed that the probiotic Bacillus spp. isolated from E. eugeniae improved the growth, feed utilization, and the disease resistance of Nile tilapia.
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Papers by Jaypee Samson
no adverse efect on the host. This study revealed the presence of potential probiotic microorganisms in E. eugeniae that are benefcial to the aquaculture industry.
Paper URL: https://www.ijtsrd.com/biological-science/allied-sciences/23724/oral-administration-of-pulverized-wood-charcoal-on-growth-feed-utilization-survival-and-waste-excretion-of-red-tilapia-oreochromis-sp/jaypee-s-samson
Drafts by Jaypee Samson
Nile tilapia (Oreochromis niloticus). Four probiotic strains of Bacillus spp. (ANSCI9, BFAR9, RM3, and RM10) were individually incorporated in the commercial diet (control) at 108 CFU g-1of feed. The experimental fish were fed at 5% of their body weight for 30 days, and subjected to a 14-day Aeromonas hydrophila challenge test afterward. The results showed the probiotic-treated groups have higher (P<0.05) average body weight (ABW) (4.51 ± 0.34 g) than the control (3.89 ± 0.17 g). The BFAR9 (2.73 ± 0.26 g) and RM10 (3.15 ± 0.30 g) showed higher (P<0.05) absolute
growth (AG) than the control (2.20 ± 0.16 g). Furthermore, RM10 had higher (P<0.05) specific growth rate (SGR) (1.60 ± 0.10 % day-1) and relative growth rate (RGR) (181.39 ± 18.16 %) than the control (SGR=1.29 ± 0.07 % day-1
; RGR=129.84 ± 9.77 %). Consequently, RM10 had
significantly lower (P<0.05) feed conversion ratio (FCR) (1.99 ± 0.13) than the control (2.60 ±
0.16). The challenge test revealed that the probiotic-treated groups have higher (P<0.05) survival (81.25 ± 9.57 %) than the control (55.00 ± 19.15 %). These results revealed that the probiotic Bacillus spp. isolated from E. eugeniae improved the growth, feed utilization, and the disease resistance of Nile tilapia.
no adverse efect on the host. This study revealed the presence of potential probiotic microorganisms in E. eugeniae that are benefcial to the aquaculture industry.
Paper URL: https://www.ijtsrd.com/biological-science/allied-sciences/23724/oral-administration-of-pulverized-wood-charcoal-on-growth-feed-utilization-survival-and-waste-excretion-of-red-tilapia-oreochromis-sp/jaypee-s-samson
Nile tilapia (Oreochromis niloticus). Four probiotic strains of Bacillus spp. (ANSCI9, BFAR9, RM3, and RM10) were individually incorporated in the commercial diet (control) at 108 CFU g-1of feed. The experimental fish were fed at 5% of their body weight for 30 days, and subjected to a 14-day Aeromonas hydrophila challenge test afterward. The results showed the probiotic-treated groups have higher (P<0.05) average body weight (ABW) (4.51 ± 0.34 g) than the control (3.89 ± 0.17 g). The BFAR9 (2.73 ± 0.26 g) and RM10 (3.15 ± 0.30 g) showed higher (P<0.05) absolute
growth (AG) than the control (2.20 ± 0.16 g). Furthermore, RM10 had higher (P<0.05) specific growth rate (SGR) (1.60 ± 0.10 % day-1) and relative growth rate (RGR) (181.39 ± 18.16 %) than the control (SGR=1.29 ± 0.07 % day-1
; RGR=129.84 ± 9.77 %). Consequently, RM10 had
significantly lower (P<0.05) feed conversion ratio (FCR) (1.99 ± 0.13) than the control (2.60 ±
0.16). The challenge test revealed that the probiotic-treated groups have higher (P<0.05) survival (81.25 ± 9.57 %) than the control (55.00 ± 19.15 %). These results revealed that the probiotic Bacillus spp. isolated from E. eugeniae improved the growth, feed utilization, and the disease resistance of Nile tilapia.