Meat derived from pasture feeding, is associated with a high level of antioxidants. Antioxidants ... more Meat derived from pasture feeding, is associated with a high level of antioxidants. Antioxidants are incorporated within cell membranes and protect tissues against oxidation from reactive oxygen species. This maintains the overall quality of meat and secondary products. This paper reviews the implications of incorporating natural antioxidants into fresh beef, focusing on the benefits of feeding cattle good quality pasture. Pasture samples typically have higher levels of a-tocopherol, b-carotene, ascorbic acid and glutathione than feedlot samples. These compounds retard lipid and protein oxidation in fresh and stored meat, and preserve the color and odor quality of beef. The significance of antioxidant enzymes is variable, because their behavior depends on individual redox status before slaughter. Understanding total antioxidant activity requires information on antioxidant and pro-oxidant status. With an abundance of pasture, Argentina has a natural advantage in producing meat with a high antioxidant value.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
Objetivos: Presentamos nuestra experiencia en el tratamiento de la estenosis suprapulmonar postéc... more Objetivos: Presentamos nuestra experiencia en el tratamiento de la estenosis suprapulmonar postécnica quirúrgica de switch arterial mediante angioplastia percutánea/stent frente a cirugía.
We present a case of Uhl's anomaly associated with atresia of the pulmonary valve. The rarity... more We present a case of Uhl's anomaly associated with atresia of the pulmonary valve. The rarity of this association is noteworthy, only three cases having previously been published. Our case presents the peculiarity of a patient with a normal tricuspid valve.
We report our experience in balloon atrial septostomy using two-dimensional echocardiography as c... more We report our experience in balloon atrial septostomy using two-dimensional echocardiography as control for the catheter placement and performance of the procedure instead of traditional fluoroscopic control. We carried out atrial septostomy in 12 consecutive neonates with age between 1 and 18 days (mean = 3.6 +/- 5 days) diagnosed as d-transposition of the great arteries (10 cases) and pulmonary atresia with intact ventricular septum (2 cases). The procedures were performed in the catheterization laboratory in 7 cases. In the five remaining cases it was done in the neonatal intensive care unit. We emphasise the good visualization of the full balloon within the left atrium and its relationship with the mitral valve and the pulmonary veins, as well as, the problems found in case number 2 for initial placement of the catheter in the left atrium. An adequate atrial septal defect was achieved in all patients with diameter between 6 and 12 mm (mean = 8.6 +/- 2 mm). There were no complica...
Archives des maladies du coeur et des vaisseaux, 1980
20 patients with endocardial cushion defects were studied. Partial forms of common atrioventricul... more 20 patients with endocardial cushion defects were studied. Partial forms of common atrioventricular canal and those associated with complex congenital malformations were not considered. The right and left ventricular inflow and outflow tracts were measured and average values of inflow tract/outflow tract were calculated for each anatomical form of the disease. We found this ratio to be significantly smaller in Group B2 compared to Group B1 of Enriquez de Salamanca's classification, the first group being considered to be a more severe congenital malformation than the second. The inflow tract/outflow tract ratio of the right ventricle ranged between 80% and 50%, with an average of 62.3% in Group B1 compared to 62% and 24% with an average of 49% in Group B2. Using Studert's test, this difference was shown to be statistically significant (p = 0.02). On the other hand, there was no correlation between the other parameters or between the severity of the anatomical lesions and the ...
Archives des maladies du coeur et des vaisseaux, 1979
Parachute deformity of the tricuspid valve was observed with a severe form of Fallot's Tetral... more Parachute deformity of the tricuspid valve was observed with a severe form of Fallot's Tetralogy with pulmonary atresia in a newborn. Signs of tricuspid stenosis (peripheral oedema, right atrial dilatation, hepatomegaly and conduction defects) were associated with the clinical signs of the principal malformation (severe hypoxaemia). To our knowledge, this is the first reported case of parachute deformity of the tricuspid valve.
Archives des maladies du coeur et des vaisseaux, 1979
Double outlet right ventricle with an intact interventricular septum is an exceedingly rare malfo... more Double outlet right ventricle with an intact interventricular septum is an exceedingly rare malformation. Only six other cases have been found in the world literature. The clinical, electrocardiographic, radiological and anatomical features of this condition were reviewed on the occasion of the diagnosis of a new case. Cardiac catheterisation and angiography performed in only one other case is not definitive in the presence of mitral atresia.
Rat fibroblasts were transfected with a plasmid containing the IE3 gene derived from the temperat... more Rat fibroblasts were transfected with a plasmid containing the IE3 gene derived from the temperature sensitive HSV-1 mutant tsK. Three of four clones expressing biologically active, temperature-sensitive ICP4, formed a substantial number of colonies in soft agar at the permissive temperature. During the first passages of cells, the transformed state of the major proportion of transformed cells was dependent on the continuous activity of ICP4. In a smaller and distinct subpopulation of transformed cells, as well as after longer subcloning of cells, ICP4 was no longer required for the maintenance of the transformed state, pointing to the induction of stable genomic changes by ICP4. Our data show that ICP4 of HSV-1 is involved in transformation of fibroblasts. Transformed cells are, however, subject to intracellular and intercellular control mechanisms.
In vitro culture results in higher oxygen concentrations than in vivo environments, leading to an... more In vitro culture results in higher oxygen concentrations than in vivo environments, leading to an increased level of reactive oxygen species (ROS) that cause lipid peroxidation of cellular membranes. Alpha-tocopherol (active form of vitamin E) is an antioxidant that protects mammalian cells against lipid peroxidation, which is regenerated by ascorbic acid. The aim of this study was to determine the effect of the addition of alpha-tocopherol and/or ascorbic acid to the maturation medium on bovine oocyte in vitro maturation (IVM) and subsequently on in vitro fertilization (IVF) and embryo development. Cumulus-oocyte complexes (COCs) were matured in Medium 199 (control), and with the addition of alpha-tocopherol and/or ascorbic acid. The concentration of alpha-tocopherol in COCs was determined by high-performance liquid chromatography (HPLC). IVF and in vitro culture (IVC) were carried out in modified synthetic oviductal fluid (mSOF). The quantity of alpha-tocopherol naturally present in COCs diminished by half during IVM (p < 0.05), although in the presence of ascorbic acid it remained constant. A greater amount of alpha-tocopherol was detected in COCs matured in medium supplemented with this antioxidant (p < 0.05), but the addition of alpha-tocopherol plus ascorbic acid maintained higher levels of alpha-tocopherol (p < 0.05). Significant differences were not observed in the percentages of nuclear maturation and fertilization among different treatments. The presence of alpha-tocopherol or ascorbic acid in the maturation medium failed to modify the percentage of blastocysts obtained, unlike the addition of both antioxidants when a significant decrease was observed (p < 0.05). Absorbic acid maintained the antioxidant capacity of the alpha-tocopherol incorporated to COC membranes during IVM. The active form of vitamin E during maturation impaired the acquisition of oocyte developmental competence.
The main goal of the present work was to determine the overall antioxidant status in fresh meat f... more The main goal of the present work was to determine the overall antioxidant status in fresh meat from animals fed different diets and to differentiate them through their odour profiles. Attributes were evaluated in beef from pasture or grain-fed animals with (PE and GE) or without supplementation (P and G) with vitamin E (500 UI/head/day).
Buffalo meat production is increasing in Argentina. Information on meat quality and nutritional v... more Buffalo meat production is increasing in Argentina. Information on meat quality and nutritional value will be useful in marketing. This work describes the oxidative stability of the Longissimus dorsi (LD) in relation to consumption of antioxidant vitamins, fatty acid composition and color deterioration during ageing. Vitamins levels found in fresh beef were 4.22 ± 0.93; 0.24 ± 0.05 and 0.25 ± 0.06 lg/ g for a-and c-tocopherol, and b-carotene, respectively. Vitamin loss was almost 90% throughout an ageing period of 25 days at 2°C. Concomitantly, TBARS levels increased from 0.076 ± 0.018 to 0.14 6 ± 0.032 mg MDA/kg beef. Hexanal and pentanal levels were low and no correlations with oxidation were detected (P > 0.05). The predominant color changes in aged beef were reduced redness and yellowness with an increase in lightness (P < 0.05). Vitamin levels and TBARS were used to develop a prediction equation for post-mortem aging.
Argentine meat has been traditionally produced on pasture. However, to comply with some market re... more Argentine meat has been traditionally produced on pasture. However, to comply with some market requirements, grain finishing is becoming more common among producers. The main goal of the present work was to study lipid oxidation in fresh meat from animals fed different diets in relationship with their antioxidant vitamin status. Attributes were evaluated in beef from pasture or grain-fed animals with (PE and GE) or without supplementation (P and G) with vitamin E (500 UI/head/day). Fresh meat produced on grain (G and GE) had higher fat (4.0 ± 1.6 and 4.7 ± 1.4 g/100 g) and cholesterol content (51.0 ± 3.0 and 52.0 ± 4.0 mg/100 g) than meat from pasture (P and PE) fed animals (2.7 ± 1.2 to 2.9 ± 1.1 g/100 g and 48.0 ± 5.0 to 49.0 ± 4.0 mg/100 g of intramuscular fat and cholesterol respectively). Fatty acid composition was clearly affected by diet. Beef from pasture-fed cattle had higher percentage of linolenic acid, less linoleic acid and, overall, higher percentage of polyunsaturated fatty acids than beef from grainfed animals (P < 0.05).
Meat derived from pasture feeding, is associated with a high level of antioxidants. Antioxidants ... more Meat derived from pasture feeding, is associated with a high level of antioxidants. Antioxidants are incorporated within cell membranes and protect tissues against oxidation from reactive oxygen species. This maintains the overall quality of meat and secondary products. This paper reviews the implications of incorporating natural antioxidants into fresh beef, focusing on the benefits of feeding cattle good quality pasture. Pasture samples typically have higher levels of a-tocopherol, b-carotene, ascorbic acid and glutathione than feedlot samples. These compounds retard lipid and protein oxidation in fresh and stored meat, and preserve the color and odor quality of beef. The significance of antioxidant enzymes is variable, because their behavior depends on individual redox status before slaughter. Understanding total antioxidant activity requires information on antioxidant and pro-oxidant status. With an abundance of pasture, Argentina has a natural advantage in producing meat with a high antioxidant value.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
Objetivos: Presentamos nuestra experiencia en el tratamiento de la estenosis suprapulmonar postéc... more Objetivos: Presentamos nuestra experiencia en el tratamiento de la estenosis suprapulmonar postécnica quirúrgica de switch arterial mediante angioplastia percutánea/stent frente a cirugía.
We present a case of Uhl's anomaly associated with atresia of the pulmonary valve. The rarity... more We present a case of Uhl's anomaly associated with atresia of the pulmonary valve. The rarity of this association is noteworthy, only three cases having previously been published. Our case presents the peculiarity of a patient with a normal tricuspid valve.
We report our experience in balloon atrial septostomy using two-dimensional echocardiography as c... more We report our experience in balloon atrial septostomy using two-dimensional echocardiography as control for the catheter placement and performance of the procedure instead of traditional fluoroscopic control. We carried out atrial septostomy in 12 consecutive neonates with age between 1 and 18 days (mean = 3.6 +/- 5 days) diagnosed as d-transposition of the great arteries (10 cases) and pulmonary atresia with intact ventricular septum (2 cases). The procedures were performed in the catheterization laboratory in 7 cases. In the five remaining cases it was done in the neonatal intensive care unit. We emphasise the good visualization of the full balloon within the left atrium and its relationship with the mitral valve and the pulmonary veins, as well as, the problems found in case number 2 for initial placement of the catheter in the left atrium. An adequate atrial septal defect was achieved in all patients with diameter between 6 and 12 mm (mean = 8.6 +/- 2 mm). There were no complica...
Archives des maladies du coeur et des vaisseaux, 1980
20 patients with endocardial cushion defects were studied. Partial forms of common atrioventricul... more 20 patients with endocardial cushion defects were studied. Partial forms of common atrioventricular canal and those associated with complex congenital malformations were not considered. The right and left ventricular inflow and outflow tracts were measured and average values of inflow tract/outflow tract were calculated for each anatomical form of the disease. We found this ratio to be significantly smaller in Group B2 compared to Group B1 of Enriquez de Salamanca's classification, the first group being considered to be a more severe congenital malformation than the second. The inflow tract/outflow tract ratio of the right ventricle ranged between 80% and 50%, with an average of 62.3% in Group B1 compared to 62% and 24% with an average of 49% in Group B2. Using Studert's test, this difference was shown to be statistically significant (p = 0.02). On the other hand, there was no correlation between the other parameters or between the severity of the anatomical lesions and the ...
Archives des maladies du coeur et des vaisseaux, 1979
Parachute deformity of the tricuspid valve was observed with a severe form of Fallot's Tetral... more Parachute deformity of the tricuspid valve was observed with a severe form of Fallot's Tetralogy with pulmonary atresia in a newborn. Signs of tricuspid stenosis (peripheral oedema, right atrial dilatation, hepatomegaly and conduction defects) were associated with the clinical signs of the principal malformation (severe hypoxaemia). To our knowledge, this is the first reported case of parachute deformity of the tricuspid valve.
Archives des maladies du coeur et des vaisseaux, 1979
Double outlet right ventricle with an intact interventricular septum is an exceedingly rare malfo... more Double outlet right ventricle with an intact interventricular septum is an exceedingly rare malformation. Only six other cases have been found in the world literature. The clinical, electrocardiographic, radiological and anatomical features of this condition were reviewed on the occasion of the diagnosis of a new case. Cardiac catheterisation and angiography performed in only one other case is not definitive in the presence of mitral atresia.
Rat fibroblasts were transfected with a plasmid containing the IE3 gene derived from the temperat... more Rat fibroblasts were transfected with a plasmid containing the IE3 gene derived from the temperature sensitive HSV-1 mutant tsK. Three of four clones expressing biologically active, temperature-sensitive ICP4, formed a substantial number of colonies in soft agar at the permissive temperature. During the first passages of cells, the transformed state of the major proportion of transformed cells was dependent on the continuous activity of ICP4. In a smaller and distinct subpopulation of transformed cells, as well as after longer subcloning of cells, ICP4 was no longer required for the maintenance of the transformed state, pointing to the induction of stable genomic changes by ICP4. Our data show that ICP4 of HSV-1 is involved in transformation of fibroblasts. Transformed cells are, however, subject to intracellular and intercellular control mechanisms.
In vitro culture results in higher oxygen concentrations than in vivo environments, leading to an... more In vitro culture results in higher oxygen concentrations than in vivo environments, leading to an increased level of reactive oxygen species (ROS) that cause lipid peroxidation of cellular membranes. Alpha-tocopherol (active form of vitamin E) is an antioxidant that protects mammalian cells against lipid peroxidation, which is regenerated by ascorbic acid. The aim of this study was to determine the effect of the addition of alpha-tocopherol and/or ascorbic acid to the maturation medium on bovine oocyte in vitro maturation (IVM) and subsequently on in vitro fertilization (IVF) and embryo development. Cumulus-oocyte complexes (COCs) were matured in Medium 199 (control), and with the addition of alpha-tocopherol and/or ascorbic acid. The concentration of alpha-tocopherol in COCs was determined by high-performance liquid chromatography (HPLC). IVF and in vitro culture (IVC) were carried out in modified synthetic oviductal fluid (mSOF). The quantity of alpha-tocopherol naturally present in COCs diminished by half during IVM (p &amp;amp;lt; 0.05), although in the presence of ascorbic acid it remained constant. A greater amount of alpha-tocopherol was detected in COCs matured in medium supplemented with this antioxidant (p &amp;amp;lt; 0.05), but the addition of alpha-tocopherol plus ascorbic acid maintained higher levels of alpha-tocopherol (p &amp;amp;lt; 0.05). Significant differences were not observed in the percentages of nuclear maturation and fertilization among different treatments. The presence of alpha-tocopherol or ascorbic acid in the maturation medium failed to modify the percentage of blastocysts obtained, unlike the addition of both antioxidants when a significant decrease was observed (p &amp;amp;lt; 0.05). Absorbic acid maintained the antioxidant capacity of the alpha-tocopherol incorporated to COC membranes during IVM. The active form of vitamin E during maturation impaired the acquisition of oocyte developmental competence.
The main goal of the present work was to determine the overall antioxidant status in fresh meat f... more The main goal of the present work was to determine the overall antioxidant status in fresh meat from animals fed different diets and to differentiate them through their odour profiles. Attributes were evaluated in beef from pasture or grain-fed animals with (PE and GE) or without supplementation (P and G) with vitamin E (500 UI/head/day).
Buffalo meat production is increasing in Argentina. Information on meat quality and nutritional v... more Buffalo meat production is increasing in Argentina. Information on meat quality and nutritional value will be useful in marketing. This work describes the oxidative stability of the Longissimus dorsi (LD) in relation to consumption of antioxidant vitamins, fatty acid composition and color deterioration during ageing. Vitamins levels found in fresh beef were 4.22 ± 0.93; 0.24 ± 0.05 and 0.25 ± 0.06 lg/ g for a-and c-tocopherol, and b-carotene, respectively. Vitamin loss was almost 90% throughout an ageing period of 25 days at 2°C. Concomitantly, TBARS levels increased from 0.076 ± 0.018 to 0.14 6 ± 0.032 mg MDA/kg beef. Hexanal and pentanal levels were low and no correlations with oxidation were detected (P > 0.05). The predominant color changes in aged beef were reduced redness and yellowness with an increase in lightness (P < 0.05). Vitamin levels and TBARS were used to develop a prediction equation for post-mortem aging.
Argentine meat has been traditionally produced on pasture. However, to comply with some market re... more Argentine meat has been traditionally produced on pasture. However, to comply with some market requirements, grain finishing is becoming more common among producers. The main goal of the present work was to study lipid oxidation in fresh meat from animals fed different diets in relationship with their antioxidant vitamin status. Attributes were evaluated in beef from pasture or grain-fed animals with (PE and GE) or without supplementation (P and G) with vitamin E (500 UI/head/day). Fresh meat produced on grain (G and GE) had higher fat (4.0 ± 1.6 and 4.7 ± 1.4 g/100 g) and cholesterol content (51.0 ± 3.0 and 52.0 ± 4.0 mg/100 g) than meat from pasture (P and PE) fed animals (2.7 ± 1.2 to 2.9 ± 1.1 g/100 g and 48.0 ± 5.0 to 49.0 ± 4.0 mg/100 g of intramuscular fat and cholesterol respectively). Fatty acid composition was clearly affected by diet. Beef from pasture-fed cattle had higher percentage of linolenic acid, less linoleic acid and, overall, higher percentage of polyunsaturated fatty acids than beef from grainfed animals (P < 0.05).
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