Papers by Mansurah Abdulazeez
African Journal of Biochemistry Research, Oct 31, 2013
This study was aimed at investigating the angiotensin converting enzyme (ACE) inhibitory effect, ... more This study was aimed at investigating the angiotensin converting enzyme (ACE) inhibitory effect, hypolidemic and antioxidant properties of combined extract of Occimum gratissimum and Vernonia amygdalina in streptozotocin-induced diabetic rats. Thirty (30) apparently healthy male Wistar rats were grouped into: control, diabetic, diabetic + insulin, diabetic + 100 mg/kg and diabetic + 250 mg/kg extract groups. All rats except those in the control group were made diabetic using a single intraperitoneal injection of 60 mg/kg streptozotocin. Administration of extract began four days after induction of diabetes and lasted for four weeks. Results show a significant (P<0.05) increase in serum and liver triacyglycerol, total cholesterol and low density lipoprotein levels upon induction of diabetes, which reduced significantly (P<0.05) in rats given insulin and the extract. HDL-C levels remained unchanged in both liver and serum of diabetic rats when compared to control rats. The serum levels of sulfuroxide dismutase (SOD) and catalase increased significantly (P<0.05) in rats given insulin and the extract, while thiobarbituric acid reactive substances decreased significantly (P<0.05) compared to diabetic rats. The angiotensin converting enzyme (ACE) activity in lungs, kidney and heart of diabetic rats were significantly (P<0.05) higher than that of control rats. Also kidney and liver functions were affected on induction of diabetes with a significant increase in levels of urea and aspartate aminotransferase (AST). All of the above factors reduced significantly (P<0.05) when rats were given either insulin or the extract. In conclusion, this study has shown that a combination of the aqueous leaf extracts of Vernonia amygdalina and Ocimum gratissimum inhibits ACE activity, lowers blood glucose and lipid levels; and is a good antioxidant.
Biomedicine & Pharmacotherapy, 2022
The plant Peristrophe bicalyculata (Retz) Nees is used for the treatment of cancer. While its lea... more The plant Peristrophe bicalyculata (Retz) Nees is used for the treatment of cancer. While its leaf extracts have been shown to inhibit the growth of some cancer cells, there is little information supporting the constituents' anti-tumour potential. This study, therefore, investigated the effects of the plant's leaf extracts on cancer cells and the associated cellular/molecular mechanisms. Extracts were prepared using hexane (PBH), chloroform (PBC), ethyl acetate (PBE) and methanol (PBM) and constituents were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Their cytotoxic effects on human cervical (HeLa) and lung cancer (MRC5-SV2) cells were assessed using the MTT and LDH release assays. Reactive oxygen species (ROS) production was assessed using 2',7'-dichlorofluorescein diacetate (DCFDA) and mitochondrial membrane potential by staining with JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide). Caspase activation was determined using a Caspase-Glo-3/7 assay, and DNA damage by the Comet assay. Changes to mRNA expression were assessed using Quantitative Real-Time PCR. PBC, PBE and PBM reduced cell viability and induced LDH release, with IC50 values (48 h, MTT, in μg/ml), respectively, of 6.21 ± 0.70, 23.39 ± 3.92, and 22.43 ± 3.58 (HeLa); and 1.98 ± 0.33, 8.57 ± 1.91 and 28.24 ± 5.57 (MRC5-SV2). PBC induced ROS, while PBC, PBE and PBM impaired mitochondrial membrane potential and induced caspase 3/7 activation. PBC and PBE induced DNA damage, and PBE induced caspase-3 mRNA expression. Constituents of the extracts included derivatives of gallic acid, dipeptides, diterpenoids and flavones. We conclude that P. bicalyculata contains cytotoxic principles that could be potential leads for developing novel anti-cancer agents.
African Journal of Biotechnology, 2009
This study was performed to determine the effects of fermented seeds of Carica papaya on litters ... more This study was performed to determine the effects of fermented seeds of Carica papaya on litters of female Wistar rats, and ascertain its safety as a food condiment. Animals in group one served as control, and were given only water; those in groups two and three were given the aqueous extract of the fermented seeds of C. papaya at 500 and 1500 mg/kg, respectively, from gestation day six to gestation day 15, kept till terms and allowed to litter. Results showed that the percentage increase in weight of rats in the control group was not significant (P and litter body weight in rats within all the groups, compared to the control group. Also, the anogenital distance (AGD) to crown rump length (CRL) ratio in rats dosed with the fermented extract of C. papaya seeds at 500 and 1500 mg/kg was not significantly different from the control group. In conclusion, this study has shown that the fermented seeds of C. papaya seeds does not affect the litters and may be safe as a food condiment.
The aqueous, butanolic and methanolic fractions of Peristrophe bicalyculata were accessed for the... more The aqueous, butanolic and methanolic fractions of Peristrophe bicalyculata were accessed for their effect on the arterial blood pressure, liver enzymes and some kidney parameters in hypertensive rats. Forty rats were divided into nine groups of five rats each: control, hypertensive control, Standard (enalapril 3.8 mg/kg), methanolic extract (100 and 200 mg/kg), butanolic extract (100 and 200 mg/kg) and aqueous extract (100 and 200 mg/kg). All rats, except control group were made hypertensive using the Goldbatt Two Kidney One Clip (2K1C) method. Administration of extracts began four weeks after induction of hypertension, and lasted for two weeks. Blood pressure was determined before inducing hypertension, and then weekly after the induction. Liver and kidney parameters were determined at the end of experiment. Results showed a significant decrease (p<0.05) in blood pressure of the rats given the standard and all extracts compared to the hypertensive control group. The serum level...
Journal of Drug Delivery and Therapeutics, May 15, 2018
The use of plant products in the management of diabetes has gained ground in pharmacotherapy. It ... more The use of plant products in the management of diabetes has gained ground in pharmacotherapy. It becomes imperative to evaluate the antidiabetic effects of fruit extracts of Ziziphus mauritiana (HMZM), Ziziphus spina christi (HMZS) and Glibenclamide on blood glucose, total protein, albumin and lipid profile in alloxan induced diabetic rats. 68 albino rats weighing 70-130g were used in this study. 26 rats were used for acute toxicities of Ziziphus mauritiana and Ziziphus spina christi. 42 rats of 7 groups of 6 rats each were used to test antidiabetic effects of Ziziphus mauritiana and Ziziphus spina christi plant extracts. Group 1 served as the negative control, groups 2-7 were intraperitoneally administered 360mg/kg of alloxan in normal saline respectively. Group 2 served as positive control, groups 3 and 4; 5 and 6 were respectively administered daily dose of 200 and 400mg/kg of HMZM and HMZS, group 7 was administered 0.21mg/kg of glibenclamide. Results showed Ziziphus mauritiana and Ziziphus spina christi non toxic at dose of 5000mg/kg. 48 hours after alloxan administration, blood glucose levels were found to be significantly higher (P<0.05) in groups 2-7 compared to group 1, thus confirming induction of diabetes. In groups 3-7, on 7 th and 14 th days of administration of extracts and Glibenclamide, there was a significantly lower (p<0.05) serum glucose, triacylglycerol, High density Lipoprotein, Low density lipoprotein and significantly higher (P > 0.05) serum level of total protein and albumin compared to group 2. The results obtained showed a significantly lower (P < 0.05) serum level of glucose. The effects of HMZM and HMZS fruit extracts on blood glucose, total protein, albumin and lipid profile was dose dependent. Conclusively, this study has demonstrated the antidiabetic effects of HMZM and HMZS with HMZS having a more pronounced effect on Blood glucose and lipid profile.
Asian Journal of Biotechnology and Bioresource Technology, 2018
Background: Alteration of redox status is a fundamental process in the manifestation of many dise... more Background: Alteration of redox status is a fundamental process in the manifestation of many diseases including malaria. Studies have demonstrated that peptides of the renin-angiotensinaldosterone system (RAAS), such as Angiotensin Converting Enzyme and Angiotensin II, play a significant role in the pathogenesis of malaria infection by inducing the generation of reactive oxygen species. The present study was aimed at determining the redox status of Plasmodium berghei-infected mice treated with captopril, an angiotensin-converting enzyme inhibitor. Methodology: Five groups of eight mice each, categorised as control (not infected with P. berghei, not treated), malaria control (P. berghei-infected, not treated), Standard control (P. berghei-infected, treated with 0.03 mg/kg of standard drug, Lonart (Arthemeter 20 mg + Lumefantrine 120 mg), captopril low dose (P. berghei-infected, treated with 0.03 mg/kg captopril) and captopril high dose (P. berghei-infected, treated at with 0.09mg/kg captopril). The mice were treated for 14 days and were sacrificed on the 15 th day. Blood samples were collected to determine the levels of reduced Original Research Article
Journal of Applied Life Sciences International, 2018
Background: Despite all efforts to establish the pathogenesis of malaria, it is far from being un... more Background: Despite all efforts to establish the pathogenesis of malaria, it is far from being understood, and resistance to existing drugs remain a problem in several strains of Plasmodium. Different complications arise during malaria infection, and among these is the alteration in haematological parameters. This study was undertaken to evaluate the effect of captopril, an angiotensin converting enzyme (ACE) inhibitor, on some haematological parameters and DNA fragmentation in blood of Plasmodium berghei-infected mice. Methodology: Forty (40) apparently healthy mice were divided into five groups of eight mice each: control (not infected, not treated), malaria control (P. berghei-infected, not treated), Standard control (P. berghei-infected, treated with 0.03 mg/kg Lonart: Artemether 20 mg, Lumefantrine 120 mg), captopril low dose (P. berghei-infected, treated with 0.03 mg/kg captopril) and captopril high dose (P. berghei-infected, treated at with 0.09 mg/kg captopril). The mice were treated for 14 days, and parasitemia monitored every other day. The mice were sacrificed on the 15 th day and blood Original Research Article Abdulazeez et al.; JALSI, 17(1): 1-7, 2018; Article no.JALSI.40708 2 obtained for biochemical analysis: plasma full blood count and DNA fragmentation. Results: Induction of malaria significantly (P&lt;0.05) decreased the red blood cell, haematocrit and platelet count, while haemoglobin level increased significantly (P&lt;0.05) in malaria control mice compared to mice in the control group. Also, the plasma mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration decreased significantly (P&lt;0.05) in malaria control group compared to the control group. Treatment with the standard drug, lonart and captopril improved all the haematological parameters, while haematological parameters of mice treated with 0.09 mg/kg of captopril were brought back to normal. With respect to DNA fragmentation, treatment of P. berghei-infected mice with lonart and captopril significantly (P&lt;0.05) increased percentage DNA fragmentation compared to mice in control and malaria control groups. Conclusion: This result shows that captopril, especially at high concentration, may play a protective role against malaria infection.
Nigerian Journal of Basic and Clinical Sciences, 2017
Background: Changes in blood cell profile were common findings in malaria. In the rural community... more Background: Changes in blood cell profile were common findings in malaria. In the rural community of Kano State, Nigeria, information on haematological changes in human malaria was scanty in spite of their role in the pathophysiology of malaria. This cross-sectional study was undertaken to determine blood cell profiles in malaria patients attending a rural hospital in malariaendemic region. Methods: Blood samples (3 ml each) were collected in EDTA-containers from 150 randomly selected outpatients attending Gaya General Hospital, screened for malaria using RDT kit (CareStart Malaria HRP 2, Access Bio Inc., USA) based on Histidine-rich protein 2 (PfHRP-2), and blood cell profiles determined using automated Sysmex haematologic analyser. Data on sociodemographics and medical history related to the study objectives, such as taking antimalarial regimen and/or haematinic, and direct involvement in blood transfusion, were obtained by questionnaire administration supplemented with oral interview.
Journal of Acute Disease, 2017
The journal implements double-blind peer review practiced by specially invited international edit... more The journal implements double-blind peer review practiced by specially invited international editorial board members.
Journal of Biologically Active Products from Nature, 2016
Abstract Angiotensin converting enzyme (ACE) plays an important physiological role in the regulat... more Abstract Angiotensin converting enzyme (ACE) plays an important physiological role in the regulation of blood pressure by converting angiotensin I to angiotensin II, a potent vasoconstrictor; hence, its inhibition is a major target in the prevention and treatment of hypertension. In this study a novel ACE inhibitory peptide from Heterobronchus bidorsalis (cat fish) was isolated, purified and characterized. The peptide was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography (sephadex G-50). The amino acid sequence of the seventeen amino acid peptide was found to be KHRDTSEPGACVMILYF with a final inhibitory activity of 0.07 μmol/ml and a purification fold of 2.93 having a percentage yield of 34 %. The peptide had an optimum temperature and pH of 37°C and 8.3, respectively. The ACE inhibitory activity of the peptide reduced in the presence of sodium, calcium, magnesium, and potassium ions, while EDTA increased it when compared to the control. Treatment with trypsin also increased the inhibitory activity of the peptide. The peptide exhibited a non-competitive-type of inhibition, indicating that it binds to a site other than the active site. In conclusion, peptides from H. bidorsalis may be promising ACE inhibitors that can serve as nutraceuticals or drugs for blood pressure regulation in hypertensive patients.
Romanian Journal of Physics, Feb 1, 2013
The anticancer activity of leaf extracts of Peristrophe bicalyculata was evaluated. Results showe... more The anticancer activity of leaf extracts of Peristrophe bicalyculata was evaluated. Results showed that the methanolic ethylacetate fraction with an IC 50 of 15.60 ± 0.52 µg/ml was potentially very toxic against human mouth epidermal carcinoma (KB) cells. After partial purification of the methanolic ethylacetate fraction, seven fractions were obtained; five of which were inactive (IC 50 values above 500 μg/ml) and one moderately toxic (IC 50 = 235 ± 33.05 µg/ml), hence not considered. The IC 50 value of the most cytotoxic fraction (fraction 5) (3.50 ± 0.21 µg/ml) was not different from that of cisplastin (3.32 ± 0.09 µg/ml), but was significantly (P<0.05) higher than the value obtained for doxorubicin (0.43 ± 0.02 µg /ml) and vincristine (0.05 ± 0.01µg/ml). The partially-purified fraction induced apoptosis in KB cells after 24 and 48 hours in a dose-dependent manner. The percentage of apoptotic cells in cultures treated with this fraction was significantly (P<0.05) higher than that in control cultures, but lower than cultures treated with cisplastin. The GC-MS analysis of the fraction indicated an abundance of andrographolide 2(3H)-furanone and aromadendrene oxide, both of which possess anticancer properties. The results provide scientific evidence supporting the anticancer activity of the methanolic ethylacetate fraction of the plant. In conclusion, the methanolic ethylacetate fraction of Peristrophe bicalyculata is a potential source of chemotherapeutic agent.
The Body Mass Indices (BMI) and serum concentrations of High Density Lipoprotein (HDL), Total Cho... more The Body Mass Indices (BMI) and serum concentrations of High Density Lipoprotein (HDL), Total Cholesterol (TC), Total Sialic Acid (TSA) and Free Sialic Acid (FSA) were determined in thirty (30) apparently healthy students of Ahmadu Bello University, Zaria. Statistical analysis showed that the serum concentrations of TSA and FSA were significantly different (p<0.05) in males and females. Also, the Atherosclerotic Indices (AI) and serum concentrations of TC in students that take alcohol were significantly higher (p<0.05) than those that do not. These concentration values were however within normal range. A positive correlation was obtained between HDL and TC in all the students except those that consume alcohol. Between TC and TSA, a positive correlation was obtained in all the students, while between BMI and TSA, it was also positive except for those that do not take alcohol. A negative relationship existed between BMI and HDL in all the students, while between BMI and TC, all the correlations were positive except in males and smokers. It was also discovered that females and students that consume alcohol in Ahmadu Bello University, Zaria, have an increased risk to cardiovascular diseases.
Acta poloniae pharmaceutica
Hypertension is an important public health issue in both developed and developing countries due t... more Hypertension is an important public health issue in both developed and developing countries due to its high incidence and morbidity. This has motivated researchers especially in developing countries to search for strategies for the treatment using different plant parts. The use of the aqueous decoction of the leaves of Peristiophe bicalyculata in the treatment of hypertension has been documented. This study was designed to carry out a bioassay-guided isolation of the antihypertensive components of the leaves of Peristrophe bicalyculata in L-NAME hypertensive rats, determine the angiotensin-converting enzyme inhibitory activity of the extracts and fractions obtained and identify the constituent(s) present. From our results, L-NAME hypertensive rats given the cold water extract had significantly (p < 0.05) lower mean arterial blood pressure (MABP) with longer duration of action than other extracts. Also, the angiotensin-converting enzyme inhibitory activity of the cold water extrac...
Essential Oils in Food Preservation, Flavor and Safety, 2016
Abstract Black pepper (Piper nigrum L.) is one of the most widely used spices, and the constituen... more Abstract Black pepper (Piper nigrum L.) is one of the most widely used spices, and the constituent parts of its essential oil contribute to its value. Although traditionally used as a culinary ingredient, fragrant aromatic, and medicine, it is presently used in the food, cosmetic, and pharmaceutical industries. The recognition of the antioxidant and antimicrobial properties of the essential oil of black pepper fostered research and proposals for its potential use as a natural food preservative. This is because existing methods of food preservation involving the use of synthetic preservatives have been reported to have undesirable effects on health and sometimes do not completely eliminate microorganisms. Essential oil of black pepper has been used to preserve orange juice and pork. Also its effect on meat spoilage organisms has been extensively studied, prompting the call for further studies and its subsequent adoption as a natural preservative.
Journal of Medical Sciences(Faisalabad), 2013
Asian Pacific Journal of Tropical Biomedicine, 2013
Anti-trypanosomal activity, Trypanosoma brucei brucei, Peristrophe bicalyculata, Lipoproteins Obj... more Anti-trypanosomal activity, Trypanosoma brucei brucei, Peristrophe bicalyculata, Lipoproteins Objective: To investigate the in vitro and in vivo effect of whole plant extracts of Peristrophe bicalyculata on Trypanosoma brucei brucei-infected rats. Methods: The experiment was divided into two phases: In the first phase, the anti-trypanosomal activity of the hot water, cold water, methanol and butanol extracts of the whole plant were determined by incubating with Trypanosoma brucei brucei. The cold water extract was partially-purified and the antitrypanosomal activity of the fractions determined. In the second phase, Trypanosoma brucei brucei-infected rats were treated with fraction 2c for nine days. Packed cell volume (PCV), high density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol (TC), triacylglycerol (TAG), aspartate aminotransferase, alanine aminotransferases (ALT), alkaline phosphatase (ALP), total and direct bilirubin levels were determined at the end of the experiment. Results: Cold water extract immobilized 90% of the parasites after 60 min of incubation, and fraction 2c completely immobilized the parasites after 35 min. It significantly increased PCV in Trypanosoma brucei brucei-infected rats. Decreased TC, TAG, HDL and LDL levels of infected rats increased significantly when rats were treated with the fraction, while elevated levels of total bilirubin and ALT also decreased. The difference in urea, direct bilirubin and ALP was not significant when infected rats were compared to rats in other groups. Conclusions: The ability of the plant to ameliorate the infection-induced biochemical changes calls for detailed investigation of the potentials of the plant for antitrypanosomiasis drug delivery.
Journal of Complementary and Alternative Medical Research, 2018
Aim: This study was carried out to determine the serum lipid profile and electrolyte level and th... more Aim: This study was carried out to determine the serum lipid profile and electrolyte level and their association with severity of malaria among patients attending Muhammadu Abdullahi Wase Specialist Hospital, Kano State, Nigeria. Methodology: Blood samples were obtained from four hundred (400) subjects: Two hundred and forty (240) pathological samples collected from malaria-infected patients and one hundred and sixty (160) from apparently healthy persons of the same age range with no evidence of malaria infection. Blood samples were screened for P. falciparum infection using both thin and thick film method, and severity of malaria was classified as described by World Health Organization. Also, the electrolyte
Journal of Ethnopharmacology
Essential Oils in Food Preservation, Flavor and Safety, 2016
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Papers by Mansurah Abdulazeez