Papers by Pracheta Janmeda
International Journal of Drug Development and Research, 2013
Flavonoids profile for the medicinally important plant Euphorbia neriifolia (Sehund) was establis... more Flavonoids profile for the medicinally important plant Euphorbia neriifolia (Sehund) was established by using high performance thin layer chromatography (HPTLC). For this direct and sequential soxhlet extraction were performed and then fractions were concentrated and subjected to HPTLC. The derivatization of developed plates was done by exposing to ammonia and iodine vapours. The plates were photo-documented at UV 366 nm and daylight using photo-documentation chamber. In the current investigation the benzene extract of E. neriifolia leaves illustrated the presence of 5 different types of flavonoids with Rf values ranging from 0.06 to 0.96. The chloroform extract of E. neriifolia demonstrated the presence of 3 to 9 different types of flavonoids with Rf values ranging from 0.03 to 0.96. The ethyl-acetate extract of E. neriifolia demonstrated the presence of 6 to 12 different types of flavonoids with Rf values ranging from 0.2 to 0.97 and ethanol, aqueous and hydro-ethanol extracts of ...
Journal of Oncology
Smoking a cigarette generates over 4000 chemicals that have a deleterious impact on each part of ... more Smoking a cigarette generates over 4000 chemicals that have a deleterious impact on each part of the human body. It produces three main severe effects on the liver organ: oncogenic, immunological, and indirect or direct toxic effects. It results in the production of cytotoxic substances, which raises fibrosis and necro-inflammation. Additionally, it also directs the production of pro-inflammatory cytokines tumour necrosis factor alfa (TNF-α) and interleukins (IL-1β, IL-6) that will be responsible for the chronic liver injury. Furthermore, it gives rise to secondary polycythemia and successively raises the turnover and mass of red cells, which might be a common factor responsible for the development of oxidative stress in the liver due to iron overload. It also produces chemicals that are having oncogenic properties and raises the risk of liver cancer especially in sufferers of chronic hepatitis C. Smoking modulates both humoral and cell-mediated responses by restricting the prolifer...
Research Journal of Biotechnology, 2021
Enhancement in the production of enzyme by utilizing different strains of microbe is one of the m... more Enhancement in the production of enzyme by utilizing different strains of microbe is one of the main prospects in biotechnology. In the present work, ethyl methane sulfonate (EMF) was selected as the chemical mutagen for inducing mutagenesis in fungi. It is a cheap method to induce random mutation as compared to other methods of recombinant technologies. Strain improvement was done by incubating the fungal spore suspension at variable concentrations of EMS i.e. 4% (v/v) and 10% (v/v) for the time period of 60, 90, and 120 min respectively. The set of control was treated with distilled water only. The fungal colonies were found to be maximum in control plate as compared to the EMF exposed plates. The number of fungal colonies was reduced as we raised the exposure time of EMF. Specific activity and the lipase activity of wild strain and hyperproducer were evaluated under the submerged (SmF) and solid-state fermentation (SSF). The wild strain denoted the 3.2 U/ml/min of enzymatic activ...
SUMMARY Nowadays, focus on plant research has increased all over the world. Alternate healthcare ... more SUMMARY Nowadays, focus on plant research has increased all over the world. Alternate healthcare system which has a growing potential as export earner because plant based medicines are affordable offer diversity and have freedom from side effects of synthetic drugs and resistance emergence to pathogenic microbes. Since Sehund is important latex producing medicinal plant of the dominant and large spurge family of Indian subcontinent, a mega-diversity center of the world, it has been chosen for the bio-prospecting of novel drugs. This review aims, (i) to refresh the importance of Sehund to the medicinal plant researchers and (ii) to summarize the folkloric uses, pharmacological activities, and phytoconstituents of Sehund for ancient times.
The Applied Biology & Chemistry Journal
Nanoparticles (1-100 nanometres in size), products of nanotechnology, offer a modern way to trans... more Nanoparticles (1-100 nanometres in size), products of nanotechnology, offer a modern way to transport anti-cancer drugs by acting as transporters of drugs into tumor cells, hence quenching tumor cell proliferation. Such nanoparticles may be formulated to bind to the tumor cell membrane or inhibit specific reactions of tumor biosynthetic pathway by gene repression, or directly bind to the active sites of essential enzymes in the biosynthetic pathway. Consequently, drugs are completely delivered to the desired cancerous cells without system interference. Liposomal doxorubicin and albumin-bound paclitaxel are two examples of nanotechnologically developed drugs for treating cancer. Modern knowledge of nanotechnology opens up new opportunities for innovative research on cancer therapies and administration and helps minimize harm to healthy cells. This review focuses on the doses and routes of administration of these chemotherapeutic agents used in treating cancers.
Environment Conservation Journal
N-nitroso compounds (NOC) are produced by the acid catalyzed reaction of nitrite with certain nit... more N-nitroso compounds (NOC) are produced by the acid catalyzed reaction of nitrite with certain nitrogen compounds. The gastrointestinal tract (GI) is the main site for the formation of reactive nitrogen species (RNS). Bacteria present on the GI tract surface reduced the dietary nitrate or nitrite into Nitric oxide NO and other related compounds. The clinical sign of NOC carcinogenicity varies according to species, dose, and route of administration. Humans are exposed to preformed N-nitroso compounds and endogenous NOC via the environmental food chain. Several NOC are potential human carcinogens, including DENA and NDMA, but evidence from population studies is inconsistent.
Evidence-Based Complementary and Alternative Medicine
The mangrove plants are the potential sources of foods and remedies for people living in the fore... more The mangrove plants are the potential sources of foods and remedies for people living in the forests and nearby communities. Xylocarpus granatum J. Koenig is traditionally used to treat various diseases including diarrhea, cholera, dysentery, fever, malaria, and viral infections, among others. To summarize critically the taxonomy, ethnomedicinal, phytochemistry, and pharmacological activities of X. granatum, information was collected from different databases. An up-to-date search (till June 2020) was carried out with the help of various scientific web resources from databases such as PubMed, Science Direct, Google Scholar, and various patent offices (e.g., WIPO, CIPO, and USPTO) using the keywords “Xylocarpus granatum” and then paired with ethnomedicinal use and phytochemical, phytochemistry, and pharmacological activity (in vitro, ex vivo, and in vivo studies). Findings revealed that seeds, fruits, stem bark, leaf, and twigs of X. granatum exhibited a wide range of key phytochemica...
Environment Conservation Journal
The latest outbreak of a respiratory disease, known as coronavirus disease 19 (COVID-19), is the ... more The latest outbreak of a respiratory disease, known as coronavirus disease 19 (COVID-19), is the third virus spillover from animals to humans in the last two decades. The disease is caused by coronavirus and has converted into an epidemic in recent days. It spread via direct contact or droplets of nasal-discharge from one human-to-another within the mean-incubation period of 6.5 days. Dyspnea, cough and fever are the most common symptoms in the patients of COVID-19, though along with diarrhea in 3% cases. Bilateral pulmonary with ground-glass opacity and consolidation has been observed in 98% cases of the disease by the help of computed tomography. The treatment process of COVID-19 with chloroquine and remdesivir drug is under the clinical trial worldwide and responding well to cure the disease. Under the prevalent circumstances, the main goal is to control the widespread infection of SARS-CoV-2 across the world and to aware the public regarding the possible preventive measures and...
Current Research in Pharmacology and Drug Discovery, 2021
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-coronavirus dise... more The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-coronavirus disease 2019 (COVID-19) has raised a severe global public health issue and creates a pandemic situation. The present work aims to study the molecular-docking and dynamic of three pertinent medicinal plants i.e. Eurycoma harmandiana, Sophora flavescens and Andrographis paniculata phyto-compounds against SARS-COV-2 papain-like protease (PL pro) and main protease (M pro)/3-chymotrypsin-like protease (3CL pro). The interaction of protein targets and ligands was performed through AutoDock-Vina visualized using PyMOL and BIOVIA-Discovery Studio 2020. Molecular docking with canthin-6-one 9-O-beta-glucopyranoside showed highest binding affinity and less binding energy with both PL pro and M pro /3CL pro proteases and was subjected to molecular dynamic (MD) simulations for a period of 100ns. Stability of the protein-ligand complexes was evaluated by different analyses. The binding free energy calcula...
Research Square, 2020
The rapid spread of SARS-CoV-2 has raised a severe global public health issue, where therapy is n... more The rapid spread of SARS-CoV-2 has raised a severe global public health issue, where therapy is not identi ed with speci c drugs or vaccines. The present investigation dealt with the inhibition of various proteins and receptors of virus using phytocompounds of three pertinent medicinal plants i.e. Eurycoma harmandiana, Sophora avescens and Andrographis paniculata. Phytocompounds known to have antiviral properties were screened against the papain-like protease (PL pro) and main protease (M pro) / 3-chymotrypsin-like Protease (3CL pro). Molecular docking with Canthin-6-One 9-O-Beta-Glucopyranoside showed the highest binding a nity and least binding energy with both the proteases viz. PL pro and M pro / 3CL pro. ADMET analysis of the compound suggested that it is having drug-like properties like high gastrointestinal (gi-) absorption, no blood-brain barrier permeability, and high lipophilicity.
Plant Science Today
Trianthema portulacastrum L. ( Biskhapra/ Horse purslane) is widely found in tropical and sub-tro... more Trianthema portulacastrum L. ( Biskhapra/ Horse purslane) is widely found in tropical and sub-tropical countries of the world. This weed automatically spread in cultivated fields. From the ancient time it is used for curative purposes. The plant pertains wide range of applicability and henceforth used as an Ayurvedic herb. The decoction of this herb utilized as a vermifuge, antidote prepared from that helps in treating alcohol poisoning and leaves cure the wound. In the era of phytomedicines lot of work has been done related to its morphology, ethno-pharmacology, medicinal uses, phyto-chemistry and pharmacological properties. Various pharmacological properties like antimicrobial properties, analgesic, anti-inflammatory, anti-diabetic, anti-hyperglycemic, hepato-protective activity makes this plant very renowned amongst researchers as they utilized it somewhat like a panacea. Different parts of plants are utilized for the therapeutic purposes and extract prepared in different solvent...
Arabian Journal of Chemistry, 2014
The flavonoids contained in Euphorbia neriifolia leaves were extracted, identified and characteri... more The flavonoids contained in Euphorbia neriifolia leaves were extracted, identified and characterized. Direct and sequential soxhlet extraction and its concentrated fractions were subjected to thin layer chromatography and high performance thin layer chromatography. The results showed that maximum yield of the flavonoid (6.53 g) was obtained from ethanolic extract. The R f value of isolated flavonoid and phytochemical screening has been compared with standard Quercetin. Characterization of isolated flavonoid was done by IR, 1 H NMR, and MS. On the basis of chemical and spectral analysis structure was elucidated as 2-(3,4-dihydroxy-5-methoxy-phenyl)-3,5-dihydroxy-6,7-dimethoxychromen-4-one, a flavonoid. This compound was isolated for the first time from this plant.
Medicinal plants are considered as a rich resource of ancient medicines and so many of them are u... more Medicinal plants are considered as a rich resource of ancient medicines and so many of them are used as a major ingredient of today's panacea that can be pharmacopoeic, non-pharmacopoeic, or synthetic drugs. They have been used to cure health disorders for the past thousand years. The ubiquitous use of folk remedies and health preparations is outlined in the Vedas and the Bible. Plant safety, quality, and efficacy assurance has now become a major issue in developing and developed countries. Cultural consequence and importance showing great demand for herbal and aromatic plants and following highlight point needed for the immense response in this field like encouraging the cultivation of medicinal plants having large market prospects. Research and evolution need to stand together for improving the manufacturing and efficiency of plants by establishing massive outcome through expanding collaboration between farmers and researchers.
SN Applied Sciences
The present study about Trianthema portulacastrum (Linn.), family Aizoaceae, is an effort to eval... more The present study about Trianthema portulacastrum (Linn.), family Aizoaceae, is an effort to evaluate physicochemical parameters, phytochemical screening, pharmacognostic properties of stem, root, leaf and seed and preparedness of different extracts by solvent system of wide-range polarity. Physicochemical analysis includes loss on dryness, total ash value, water-soluble ash value, acid-insoluble ash value, pH analysis, fluorescence study and extractive values in different solvent systems. All the parts of the plant showed loss on dryness in the range of 2 ± 0.69 to 7 ± 0.73. Foreign organic matters recorded in different parts of plant were in the range of 0.8 ± 0.045 to 2.0 ± 0.081. The total ash values were determined from 9.59 ± 1.87 to 15.54 ± 1.21% (w/w). The water-soluble ash values were from 7.5 ± 1.10 to 9.38 ± 1.54% (w/w), and acid-insoluble ash values were from 0.92 ± 0.84 to 4.19 ± 0.75% (w/w). Macroscopic, powder microscopy, fluorescence studies, extractive values, different properties of extracts and phytochemical screening studies were completed by a series of standard tests. The parameters evaluated under this study will safeguard the authenticity and efficacy of crude drug and provide referential information in distinguishing the drug from its adulterants. The observations of studied parameters will be useful and helpful in setting diagnostic indices for identification and preparation of monograph of this plant and in differentiating root, stem, leaf and seed of this species from closely related species of same genus and family.
Reactive oxygen species (ROS) is a metabolic side product of oxidative stress process, which caus... more Reactive oxygen species (ROS) is a metabolic side product of oxidative stress process, which causes several diseases like atherosclerosis, cancer, etc. In defense of ROS, antioxidants play a key role in combating them. As the process of aging increases, the level of antioxidants in our body decreases and thereby needs utmost attention for its repair process, which is generally administered externally. Plant products serve a best source for controlling these activities by its own metabolic pathway. Studies on the antioxidant activities of Maytenus emarginata leaf extracts are lacking. Antioxidant activity of the methanol extract of Maytenus emarginata was determined by DPPH free radical nitric oxide scavenging assays, superoxide ion scavenging assays, ABTS, and iron chelating methods. Preliminary phytochemical screening revealed that the extract of Maytenus emarginata leaves possesses phenols, flavonoids, steroids, glycosides, saponins, tannins, and triterpenoids. The extract showed significant activities in all antioxidant assays compared to the standard antioxidant (ascorbic acid) in a dose-dependent manner, and remarkable activities to scavenge ROS may be attributed by the presence of the above active compounds in the leaves. The amount of total phenolics and flavonoid contents were also estimated. The DPPH, ABTS, Nitric oxide, superoxide, and iron chelating IC(50) values of the methanolic extracts were 12.44, 24.27, 22.41, 5.85, and 2.74 μg/mL, respectively. The total phenolic content of the methanolic extract was 10.69 mg CA/g, whereas the total flavonoid was 1.56 mg CAE/g. The antioxidant activities were correlated with the total phenolic content. This result suggests that the relatively high antioxidant activity of the methanolic extract compared to standard could be possibly be due to its high phenolic content.
sphinxsai.com
India is one of the 12 mega-diversity countries. It is estimated that around 70,000 plant species... more India is one of the 12 mega-diversity countries. It is estimated that around 70,000 plant species, approximately 7500 species have been recorded to have medicinal value. The 300 species are used by 7800 medicinal drug manufacturing units in India, which consume about 2000 tons of herbs annually. There are estimated to be more than 717,319 registered practitioners of ayurveda, siddha, unani and homeopathy in India and in recent years, the growing demand for herbal products has led to the extinction of many important plant herbs. Ashwagandha is also a rare and endangered plant. Withania somnifera possess good immunomodulatory anti-inflammatory, anti-tumor, antioxidant, anticancer properties and many pharmacologically and medicinally important chemicals, they protect the cells from oxidative damage and diseases. In present paper we have tried to unveil the therapeutic knowledge about Ashwagandha, which is used to exploit novel medicines. Considering its relevance, further research is required to explore the potential from this medicinal herb.
Asian Journal of Biotechnology
Asian Pacific journal of …, Jan 1, 2011
The present study was conducted to investigate the chemopreventive effects of hydro-ethanolic ext... more The present study was conducted to investigate the chemopreventive effects of hydro-ethanolic extract of Euphorbia neriifolia (EN) on N-nitrosodiethylamine (DENA) induced renal cancer in male Swiss albino mice. Animals were pretreated with EN extract (150 and 400 mg/kg body weight; p.o) and butylated hydroxyanisole (BHA) as a standard (0.5% and 1% BHA p.o) both for two week prior to the administration of single dose of DENA (50 mg/kg body weight; p.o). Various in vivo antioxidant biochemical parameters like lipid peroxidation (LPO), superoxide dismutase (SOD), and catalase (CAT) were evaluated to determine the reno-protective and antioxidant activity of EN. DENA increased oxidative stress through increase in LPO and decrease in antioxidant enzymes (SOD, and CAT). The EN extract significantly restored the antioxidant enzyme level in the kidney and exhibited significant dose dependant protective effect against DENA induced nephrotoxicity, which can be mainly attributed to the antioxidant property of the extract. This study rationalized the ethno-medicinal use of EN for protection against renal cancer.
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Papers by Pracheta Janmeda