Hazem Orabi

Assiut University, Urology, Faculty Member

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Vivekanand Jha

Fortis Escorts Heart Institute

Javid Dar

King Saud University

Seter Siziya

Copperbelt University

Samir Orabi

Alexandria University

Fezal Ozdemir

Ege University

Juanita Crook

University of British Columbia

Gouda M El-labban

Suez Canal University

Richard Matasic

University of medicine Zagreb

Anthony McCall

University of Virginia

Anurag Srivastava

All India Institute of Medical Sciences, New Delhi

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Papers by Hazem Orabi

Urology and Nephrology: Regenerative Medicine Applications

Encyclopedia of Biomedical Engineering, 2019

Utilization of focal therapy for patients discontinuing active surveillance of prostate cancer: Recommendations of an international Delphi consensus

Urologic Oncology: Seminars and Original Investigations, 2021

BACKGROUND With the advancement of imaging technology, focal therapy (FT) has been gaining accept... more BACKGROUND With the advancement of imaging technology, focal therapy (FT) has been gaining acceptance for the treatment of select patients with localized prostate cancer (CaP). We aim to provide details of a formal physician consensus on the utilization of FT for patients with CaP who are discontinuing active surveillance (AS). METHODS A 3-stage Delphi consensus on CaP and FT was conducted. Consensus was defined as agreement by ≥80% of physicians. An in-person meeting was attended by 17 panelists to formulate the consensus statement. RESULTS Fifty-six respondents participated in this interdisciplinary consensus study (82% urologist, 16% radiologist, 2% radiation oncology). The participants confirmed that there is a role for FT in men discontinuing AS (48% strongly agree, 39% agree). The benefit of FT over radical therapy for men coming off AS is: less invasive (91%), has a greater likelihood to preserve erectile function (91%), has a greater likelihood to preserve urinary continence (91%), has fewer side effects (86%), and has early recovery post-treatment (80%). Patients will need to undergo mpMRI of the prostate and/or a saturation biopsy to determine if they are potential candidates for FT. Our limitations include respondent's biases and that the participants of this consensus may not represent the larger medical community. CONCLUSIONS FT can be offered to men coming off AS between the age of 60 to 80 with grade group 2 localized cancer. This consensus from a multidisciplinary, multi-institutional, international expert panel provides a contemporary insight utilizing FT for CaP in select patients who are discontinuing AS.

Immunocompetent Human 3D Organ-Specific Hormone-Responding Vaginal Mucosa Model of HIV-1 Infection

Tissue Engineering Part C: Methods, 2021

The lack of appropriate experimental models often limits our ability to investigate the establish... more The lack of appropriate experimental models often limits our ability to investigate the establishment of infections in specific tissues. To reproduce the structural and spatial organization of vaginal mucosae to study human immunodeficiency virus type-1 (HIV-1) infection, we used the self-assembly technique to bioengineer tridimensional vaginal mucosae using human cells extracted from HIV-1-negative healthy pre- and postmenopausal donors. We produced a stroma, free of exogenous material, that can be adapted to generate near-to-native vaginal tissue with the best complexity obtain with seeded epithelial cells on the organ-specific stroma. The autologous engineered tissues had mechanical properties close to native mucosa and shared similar glycogen production, which declined in reconstructed tissues of the postmenopausal donor. The in vitro engineered tissues were also rendered immune-competent by adding human monocyte-derived macrophages on the epithelium or in the stroma layers. The model was infected with HIV-1, and viral replication and transcytosis were observed when immunocompetent reconstructed vaginal mucosa tissue has incorporated Monocyte-Derived Macrophages in the stroma and infected with free HIV-1 GFP viral particles. These data illustrate a natural permissiveness of immunocompetent untransformed human vaginal mucosae to HIV-1 infection. This model offers a physiological tool to explore viral load, HIV-1 transmission in an environment that may contribute to the virus's propagation, and new anti-viral treatments in vitro.

Scaffolds for tissue engineering of the urethra

Handbook of Tissue Engineering Scaffolds: Volume Two, 2019

Abstract Urethra is the site of many congenital and acquired diseases, and it requires reconstruc... more Abstract Urethra is the site of many congenital and acquired diseases, and it requires reconstruction with different tissue transfer. The lack of available tissues and complications associated with their harvest paved the way for urethral bioengineering to emerge as future for complex and long urethral segment replacement. Tissue engineering of urethra includes two main approaches: acellular and cell-seeded scaffolds. Urethral scaffolds are the cornerstone of urethral tissue engineering. In this chapter, we will discuss types of urethral scaffolds, bioengineering strategies, advancements, and challenges and clinical trials in this growing field.

Red Blood Cell Distribution Width Is Associated with All-cause Mortality but Not Adverse Cancer-specific Outcomes in Men with Clinically Localized Prostate Cancer Treated with Radical Prostatectomy: Findings Based on a Multicenter Shared Equal Access Regional Cancer Hospital Registry

European Urology Open Science, 2022

Molecular biomarkers in the context of focal therapy for prostate cancer: recommendations of a Delphi Consensus from the Focal Therapy Society

Minerva Urology and Nephrology, 2021

A lagarta Helicoverpa armigera ocorre desde a emergência das plantas até a fase reprodutiva da cu... more A lagarta Helicoverpa armigera ocorre desde a emergência das plantas até a fase reprodutiva da cultura da soja, podendo causar perdas expressivas. Como praga inicial, podem ser encontradas tanto lagartas neonatas como lagartas mais desenvolvidas, estas últimas provenientes da cultura anterior. O uso de cultivares Bt e de inseticidas em tratamento de sementes são alternativas para manejo da praga no início do desenvolvimento da soja, porém existem dúvidas sobre a eficácia destes métodos de controle. Dessa forma, objetivou-se avaliar o efeito protetivo de inseticidas aplicados às sementes e da proteína Cry 1Ac, em relação ao ataque da lagarta H. armigera, como praga inicial em plantas de soja. Experimentos foram conduzidos em laboratório, em delineamento inteiramente casualizado, com seis tratamentos: quatro inseticidas aplicados às sementes e testemunha (sem inseticida), em soja cultivar convencional (BMX Alvo), e a proteína Cry 1Ac em soja cultivar Bt (DM 5958 IPRO), sem inseticida. Os resultados comprovam que, em condições de laboratório, os inseticidas ciantraniliprole (60 g i.a./100 kg de sementes) e clorantraniliprole (62,5 g i.a./100 kg de sementes) protegem a soja em relação às injúrias das lagartas de H. armigera de 3º-4º ínstares, em cotilédones e folhas unifolioladas e trifolioladas, aos 5 dias após a emergência das plantas. A proteína Cry 1Ac protege as plantas de soja desse tipo de injúria causado tanto por lagartas de 3º-4º e como 5º-6º ínstares, aos 5 e 10 dias após a emergência. Palavras-chave: controle de pragas; diamidas antranílicas; Helicoverpa armigera; SOJA BT.

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Oncological and Functional Outcomes After Salvage Prostate Cryotherapy For The Management of Primary Brachytherapy Versus Cryotherapy Failures: A Propensity Score Matched Comparison

Cryobiology, 2021

Comparative Study Between Salvage Cryoablation of The Prostate After Primary Radiotherapy Failure and After Primary Cryotherapy Failure For Clinically Localized Prostate Cancer

Cryobiology, 2021

INTRODUCTION AND OBJECTIVE: Salvage cryoablative therapy for biopsy-proven recurrence after prima... more INTRODUCTION AND OBJECTIVE: Salvage cryoablative therapy for biopsy-proven recurrence after primary radiation or ablation treatment failure for clinically localized prostate cancer (PCa) is common. In this observational study, we aim to explore biochemical recurrence-free survival when salvage cryotherapy is performed after primary cryotherapy failure versus primary external beam radiotherapy failure in patients initially presenting with clinically localized prostate cancer. METHODS: After IRB approval, data for patients treated with salvage cryotherapy after primary external beam radiotherapy (group A) or after primary cryotherapy (group B) were collected from The Duke Prostate Cancer database and the Cryo On-Line Data (COLD) registry. Biochemical recurrence (BCR), using Phoenix criteria, after 2 and 5 years were the primary oncological outcomes. Urinary incontinence, erectile dysfunction, fistula and retention were considered as secondary functional outcomes and were assessed 12 months after salvage cryotherapy. We estimated the association between treatment and biochemical progression-free survival (BPFS) using inverse probability weighted (IPTW) Cox proportional hazards regression. Propensity score analysis, adjusting for Gleason, risk, and PSA, was implemented to account for non-random assignment of primary treatment. To test for differences in the secondary functional outcomes between treatment modalities we used Pearson's c test or Fishers exact test, corrected for IPTW. RESULTS: A total of 515 unweighted subjects met inclusion criteria and had complete data for the primary analysis.Those in group B showed an increase in 2-year BCR (HR 1.94; 95% CI, 1.1e1.34) when compared to group A. The same association was seen in 5-year BCR (HR: 1.66; 95% CI,1.01-2.72).There was no statistical difference between the 2 groups regarding the functional outcomes, although incontinence and erectile dysfunction was higher group A than group B. CONCLUSIONS: Risk of biochemical recurrence for salvage cryotherapy after primary cryoablation is higher than following primary external beam radiotherapy. This should be taken in consideration when selecting primary in-situ therapy for PCa. Further work with larger number of patients is needed to assess reasons for failure including whether they are in field or out of field of treatment.

Considerations of germline testing in prostate cancer screening

The Canadian journal of urology, 2019

Prostate cancer screening remains controversial in the medical field. While screening men above 5... more Prostate cancer screening remains controversial in the medical field. While screening men above 50 years can impose overdiagnosis and overtreatment, targeted screening of males with pathologic variants of genetic mutations is evolving and viewed as sensible. Identifying such patients requires genetic testing in males having family history of prostate cancer or certain ethnicity. Such strategies will likely occur as routine practice once favorable results of ongoing studies assessing genetic predisposition are released.

MP70-13 COMPARATIVE Study Between Salvage Cryoablation of the Prostate After Primary Radiotherapy Failure and After Primary Cryotherapy Failure for Clinically Localized Prostate Cancer

Journal of Urology, 2020

Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model

Frontiers in Bioengineering and Biotechnology, 2021

The use of autologous tissue grafts for tunica albuginea repair in Peyronie’s disease and congeni... more The use of autologous tissue grafts for tunica albuginea repair in Peyronie’s disease and congenital chordee is often restricted by limited tissue availability and donor site morbidity, therefore new biomaterial options are needed. In this study, bi-layer silk fibroin (BLSF) scaffolds were investigated to support functional tissue regeneration of tunica albuginea in a rabbit corporoplasty model. Eighteen adult male, New Zealand white rabbits were randomized to nonsurgical controls (NSC, N = 3), or subjected to corporoplasty with BLSF grafts (N = 5); decellularized small intestinal submucosa (SIS) matrices (N = 5); or autologous tunica vaginalis (TV) flaps (N = 5). End-point evaluations were cavernosography, cavernosometry, histological, immunohistochemical, and histomorphometric assessments. Maximum intracorporal pressures (ICP) following papaverine-induced erection were similar between all groups. Eighty percent of rabbits repaired with BLSF scaffolds or TV flaps achieved full rigi...

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Abstracts - USICON 2018

CT-Assisted Virtual Endoscopy: An Innovative Tool for Detecting Tissue Pathologies

The Journal of Urology, 2008

THE JOURNAL OF UROLOGY ® 679 more reliable than the examination supine as demonstrated by the gre... more

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1007 Immediate and Delayed Intracavernous (Ic) Injection of Adipose-Derived Stromal Vascular Fraction (SVF) Enhance Recovery of Erectile Function in Cavernous Nerve (CN) Injured Rats

The Journal of Urology, 2012

1014 Adipose Derived Stem Cells Ameliorate Diabetic Erectile Dysfunction in Three Dimensional Culture Model of Cavernous Tissue

The Journal of Urology, 2013

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20 Tissue Engineering Smooth Muscle from Adipose Tissue Derived Stem Cells for Genitourinary Reconstruction

The Journal of Urology, 2013

stain, and transmission electron microscopy (TEM). Finally, differentiated (4.5ϫ10 5 cells) and n... more stain, and transmission electron microscopy (TEM). Finally, differentiated (4.5ϫ10 5 cells) and non-induced USC (4.5ϫ10 5 cells) were seeded on collagen-coated inserts for barrier function assessmenton day 7 and 14. RESULTS: Morphologically, urothelial differentiated USC formed cobblestone-like cells. These differentiated USCs expressed transcripts and proteins of urothelial-specific (Uroplakin-III,-Ia) and general epithelial cell markers (CK7, CK13, CK20 and AE1/AE3) in a time-dependent manner. Expression of these markers also depended on the amount of EGF in the media. In an assay of barrier function, urothelial differentiated USC expressed tight junction gene and protein markers (ZO-1 and E-cadherin). TEM demonstrated the ultrastructural aspects of urothelial differentiated USC, including tight junction formation between neighboring cells. Using a fluorescent tracer on differentiated cells cultured on inserts, at least a 50% decrease in leakage of the tracer across the insert occurred after 3h in vitro, indicating that these cells could perform a barrier function similar to the normal urothelial cells. CONCLUSIONS: USC can be efficiently differentiated into functional urothelial cells with barrier microstructures, and these cells may be a potential cell source for tissue-engineered urethras.

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Association of lower urinary tract symptoms and erectile dysfunction: pathophysiological aspects and implications for clinical management

International Journal of Impotence Research, 2011

There is strong evidence from multiple epidemological studies that lower urinary tract symptoms (... more There is strong evidence from multiple epidemological studies that lower urinary tract symptoms (LUTS) and erectile dysfunction (ED) are correlated, independent of age or comorbidities as diabetes or hypertension. Although a direct causal relationship is not established yet, four pathophysio logical mechanisms can explain the relationship. These include alteration in nitric oxide bioavailability, a1-adrenergic receptor hyperactivity, pelvic atherosclerosis and sex hormones. This association has different clinical implications on the management of both disorders. Men seeking care for one condition should always be screened for complaints of the other condition. Sexual function should be assessed and discussed with the patient when choosing the appropriate management strategy for LUTS, as well as when evaluating the patient's response to treatment. Multiple large clinical trials have shown an improvement in LUTS after phosphodiesterase-5 (PDE5)-inhibitor treatment. PDE5 inhibitors show promise as a future treatment for LUTS, either in conjunction with existing therapies or as a primary treatment. There may be a potential therapeutic role for testosterone in LUTS treatment in cases of testosterone deficiency that needs to be investigated. Much further investigation is required, but it is evident that the association between LUTS and ED is fundamental for future therapies and possible preventative strategies.

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$Research paper thumbnail of RE Re Do urethroplasty after multiple failed surgeries of pelvic fracture urethral injury$

RE Re Do urethroplasty after multiple failed surgeries of pelvic fracture urethral injury

World Journal of Urology, 2019

We quantify surgical success rate in the management of pelvic fracture urethral injury (PFUI) wit... more We quantify surgical success rate in the management of pelvic fracture urethral injury (PFUI) with repeat urethroplasty in the setting of two or more failed prior urethroplasties. A retrospective analysis was completed of a single surgeon urethroplasty database from Jan 1, 2012 to June 31, 2018. Patients with a history of PFUI recurrent urethral stricture despite two or more failed prior urethroplasty procedures were included. We identified 87 patients that had two more more failed prior urethroplasties. These had 2 main categories. One requiring anastomotic urethroplasty and other requiring substitution urethroplasty. Total success rate was 74.75% for anastomotic group and 84.61% for substitution group with a median follow-up of 34 months (range 6–60). Overall success rate for re redo Urethroplasty was 82.70%. Bulbar urethral ischemic necrosis was identified in 14 of 64 patients (21.9%). In these cases urethral substitution measures were performed including 12 with preputial flap and tubularization, 1 sigmoid colon substitution, medial thigh flap. No significant difference was observed between the success or failure group with respect to age, BMI, stricture length, number of prior urethroplasty procedure or endoscopic procedures or comorbidities. Our findings demonstrate that high success rates can be achieved for repeat urethroplasty in recurrent PFUI urethral stricture after two or more failed prior urethroplasty procedures. Bulbar urethral ischemic necrosis is a common finding in this patient population. Patients should be managed at a tertiary high volume referral center.

ASCs characteristics and prospectives for regenerative medicine

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Tissue Engineering in Urethral Reconstruction

Textbook of Male Genitourethral Reconstruction

Urethra is the site of many congenital and acquired diseases and it requires reconstruction with ... more Urethra is the site of many congenital and acquired diseases and it requires reconstruction with different tissue transfer. The lack of available tissues and complications associated with their harvest opened the venue for tissue engineering of urethra to emerge as a hope for those cases needing extensive urethral reconstruction. Tissue engineering of urethra adopts 2 main approaches: acellular and cell-seeded scaffolds. Urethral scaffolds are the cornerstone of urethral tissue engineering. We will discuss in this chapter updates regarding urethral scaffolds, bioengineering strategies and clinical trials in this growing field.

Urology and Nephrology: Regenerative Medicine Applications

Encyclopedia of Biomedical Engineering, 2019

Utilization of focal therapy for patients discontinuing active surveillance of prostate cancer: Recommendations of an international Delphi consensus

Urologic Oncology: Seminars and Original Investigations, 2021

Immunocompetent Human 3D Organ-Specific Hormone-Responding Vaginal Mucosa Model of HIV-1 Infection

Tissue Engineering Part C: Methods, 2021

Scaffolds for tissue engineering of the urethra

Handbook of Tissue Engineering Scaffolds: Volume Two, 2019

European Urology Open Science, 2022

Molecular biomarkers in the context of focal therapy for prostate cancer: recommendations of a Delphi Consensus from the Focal Therapy Society

Minerva Urology and Nephrology, 2021

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Oncological and Functional Outcomes After Salvage Prostate Cryotherapy For The Management of Primary Brachytherapy Versus Cryotherapy Failures: A Propensity Score Matched Comparison

Cryobiology, 2021

Comparative Study Between Salvage Cryoablation of The Prostate After Primary Radiotherapy Failure and After Primary Cryotherapy Failure For Clinically Localized Prostate Cancer

Cryobiology, 2021

Considerations of germline testing in prostate cancer screening

The Canadian journal of urology, 2019

MP70-13 COMPARATIVE Study Between Salvage Cryoablation of the Prostate After Primary Radiotherapy Failure and After Primary Cryotherapy Failure for Clinically Localized Prostate Cancer

Journal of Urology, 2020

Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model

Frontiers in Bioengineering and Biotechnology, 2021

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Abstracts - USICON 2018

CT-Assisted Virtual Endoscopy: An Innovative Tool for Detecting Tissue Pathologies

The Journal of Urology, 2008

THE JOURNAL OF UROLOGY ® 679 more reliable than the examination supine as demonstrated by the gre... more

Download

1007 Immediate and Delayed Intracavernous (Ic) Injection of Adipose-Derived Stromal Vascular Fraction (SVF) Enhance Recovery of Erectile Function in Cavernous Nerve (CN) Injured Rats

The Journal of Urology, 2012

1014 Adipose Derived Stem Cells Ameliorate Diabetic Erectile Dysfunction in Three Dimensional Culture Model of Cavernous Tissue

The Journal of Urology, 2013

Download

20 Tissue Engineering Smooth Muscle from Adipose Tissue Derived Stem Cells for Genitourinary Reconstruction

The Journal of Urology, 2013

Download

Association of lower urinary tract symptoms and erectile dysfunction: pathophysiological aspects and implications for clinical management

International Journal of Impotence Research, 2011

Download

$Research paper thumbnail of RE Re Do urethroplasty after multiple failed surgeries of pelvic fracture urethral injury$

RE Re Do urethroplasty after multiple failed surgeries of pelvic fracture urethral injury