Amylase is exploited in textile, leather, wood, paper and detergent industries for desizing and w... more Amylase is exploited in textile, leather, wood, paper and detergent industries for desizing and washing of fabrics. Previously, it has been immobilized on to various compatible supports by several methods to increase its thermal and storage stability. Its immobilization onto bovine serum albumin (BSA) was found to be very significant to prepare its BSA loaded nanoparticles (BSANPs). In this study, Pearl millet amylase loaded jasmine oil driven BSA nanoparticles (BSANPs) were used and made bio-active with 35U of alkaline protease for their biodegradation. Then, these bio-active enzyme-BSANPs were used as nano-wool shampoo additives with chosen wool shampoo named, Wool & Cashmere Shampoo to test their stain removal efficacy from stained woolen fabric without affecting the delicacy and softness of the chosen fabric. In this study, chosen stains were comprised of daily routine stains of boot polish and hair color dye cream named, Kiwi Liquid boot polish Black and Loreal Paris Casting Crème Gloss Black respectively. Sometimes, these stains are very difficult to remove from the woolen fabric or required couple of pre-washing practices. So, keeping this domestic or industrial washing problem, selected stains are chosen very thoughtfully. And, from this study, an effective washing data was carried out to wash off the selected stains form woolen/cashmere/merino/mohair fabric when washed with chosen wool shampoo along with bio-active prepared jasmine oil driven amylase bound BSANPs solution as compared their washing with chosen wool shampoo alone.
Proteases are enzymes with highly specialized proteolytic functions. They are ubiquitous in occur... more Proteases are enzymes with highly specialized proteolytic functions. They are ubiquitous in occurrence, being found in all living organisms, and are essential for cell growth and differentiation. They not only have several physiological functions and roles in the living beings but are also of great importance in various industries as well thus providing a lot of economic benefits.
Alkaline phosphatase was isolated and purified from Solanum tuberosum (potato tubers) by a stream... more Alkaline phosphatase was isolated and purified from Solanum tuberosum (potato tubers) by a streamline method without the use of proteolytic and lipolytic enzymes and butanol. The study features are the use of a buffer solutions, ammonium sulphate precipitation at low temperature to remove impurity and the use of polyethylene glycol solution with potassium phosphate solution to concentrate the partially purified Solanum tuberosum , Colocasia esculenta (Taro), Punica granatum (pomegranate) and Phaseolus vulgaris (French beans) alkaline phosphatase by bi-phase extraction method. Enzyme activity of the extracted & purified Solanum tuberosum, Colocasia esculenta (Taro), Punica granatum (pomegranate) and Phaseolus vulgaris (French beans) alkaline phosphatase was done by para-nitro-phenyl-phosphate method (p-NPP method) and absorbance was taken at 410nm.
Alkaline phosphatise (ALP, ALKP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phos... more Alkaline phosphatise (ALP, ALKP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. This enzyme serves many important roles in human beings and also in various industries and therefore has a wide range of applications. This review aims to study this enzyme in detail laying special emphasis on it's mechanism, sources and industrial applications.
In present work, Cicer arietinum amylase was encapsulated by emulsification through covalent coup... more In present work, Cicer arietinum amylase was encapsulated by emulsification through covalent coupling by glutaraldehyde into chemically modified bovine serum albumin. Biodegradation of coconut oil driven emulsified bovine serum albumin encapsulated Cicer arietinum amylase was carried out by the alkaline protease for its controlled and sustained release of encapsulated enzyme from prepared microparticles of encapsulated Cicer arietinum amylase and its stability increased up to 6 months as compared to free enzyme. Its biodegradation was carried out by the using different concentration of alkaline protease (5U, 10U, 15U, 20U, 25U, 30U, 35U, 40U). Further, this coconut oil driven chemically modified bovine serum albumin microparticles of encapsulated Cicer arietinum amylase with alkaline protease were used with detergents for washing of stained cloths which have rust, gel pen ink, grease and chocolate strains. These chosen strains are very commonly present on uniforms of school going ch...
Amylase is known for starch saccharification and catalyses the starch hydrolysis producing glucos... more Amylase is known for starch saccharification and catalyses the starch hydrolysis producing glucose and limit dextrin which has wide range of applications in fermentation, textile, leather, detergents, food and paper industries. In the present work, amylase was extracted from Pennisetum glaucum (pearl millet) was entrapped in bovine serum albumin (BSA) nanoparticles in which emulsification was done by mustard oil and covalently coupled by using well known cross linking agents, glutaraldehyde. Bovine serum albumin is considered the most biodegradable, biocompatible and non-toxic biological matrix material for the preparation of enzyme bound nanospheres to enhance thermal stability, storage stability and suitability of bound enzymes as compared to free enzymes toward persisting ambient conditions. Formation of nanoparticles was confirmed by Dynamic Light scattering (DLS) and determination of exact size was observed by Scanning Electron Microscopy (SEM). Thermal stability of entrapped P...
Nanotechnology is the multidisciplinary emerging advanced technology that has vast array and pote... more Nanotechnology is the multidisciplinary emerging advanced technology that has vast array and potential to revolutionize the society
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
In present work, Cicer arietinum amylase was encapsulated into coconut driven emulsified bovine s... more In present work, Cicer arietinum amylase was encapsulated into coconut driven emulsified bovine serum albumin nanoparticle through glutaraldehyde. Biodegradation of coconut oil driven emulsified bovine serum albumin encapsulated Cicer arietinum amylase was carried out by using different units of alkaline protease (5U, 10U, 15U, 20U, 25U, 30U, 35U, 40U) to study their controlled and sustained release of encapsulated enzyme from prepared nanoparticles and hence, storage stability was enhanced up to 8 months as compared to free enzyme. Further, this coconut oil driven emulsified bovine serum albumin nanoparticles of encapsulated Cicer arietinum amylase with alkaline protease were used with detergents (aerial, surf excel, wheel and tide) for washing of stained cloth pieces which have coffee, tea, Pomegranate and turmeric stains. These chosen strains are very Common in our daily life and are very tough upon drying, hence, not to be easily washed off with well-known brand detergents upon ...
Amylase plays vital role in food, textile, leather, pharmaceutical and detergent industries due t... more Amylase plays vital role in food, textile, leather, pharmaceutical and detergent industries due to its efficacy and industrial viability. In our work, amylase was extracted from Pearl millet (Pennisetum glaucum) and encapsulated in bovine serum albumin nanoparticles by chemical modification with almond oil, n-butanol and glutaraldehyde. Hence, bovine serum albumin was found to be favourable matrix for encapsulating extracted amylase due to its well known exploitable properties e.g. biocompatibility, non-antigenicity and non-toxicity. Biodegradation of almond oil driven enzyme loaded bovine serum albumin nanoparticles was performed by using different units of alkaline protease () to study controlled release of encapsulated amylase. Kinetic parameters were also studied for Pearl millet extracted amylase and encapsulated amylase for their comparative analysis for optimal pH, incubation time, substrate concentration, CaCl 2 concentration and temperature for maximal enzyme activity which...
Targeted drug delivery is anadvanced method of delivering drugs to the patients in such a targete... more Targeted drug delivery is anadvanced method of delivering drugs to the patients in such a targeted sequences that increases the concentration of delivered drug to the targeted body part of interest only (organs/tissues/ cells) which in turn improves efficacy of treatment by reducing side effects of drug administration. Basically, targeted drug delivery is to assist the drug molecule to reach preferably to the desired site. The inherent advantage of this technique leads to administration of required drug with its reduced dose and reducedits side effect. This inherent advantage of targeted drug delivery system is under high consideration of research and development in clinical and pharmaceutical fields as backbone of therapeutics & diagnostics too. Various drug carrierwhich can be used in this advanced delivery system are soluble polymers, biodegradable microsphere polymers (synthetic and natural), neutrophils, fibroblasts, artificial cells, lipoproteins, liposomes, micelles and immune micelle. The goal of a targeted drug delivery system is to prolong, localize, target and have a protected drug interaction with the diseased tissue.
Amylases are enzymes which hydrolyzes starch as reserve carbohydrate in plants and glycogen as re... more Amylases are enzymes which hydrolyzes starch as reserve carbohydrate in plants and glycogen as reserve carbohydrate in animals into reducing fermentable sugars, mainly maltose, and reducing limit dextrins. Amylases are classified as saccharifying (β-amylase) and as dextrinizing (α-amylases). The α-amylases are found in all types of organs and tissues, whereas β-amylase is found almost exclusively in higher plants such as in seeds, cereals, yeasts, molds, and bacteria. The highest concentrations of amylase are found in the saliva and pancreas in animals. Salivary amylase is also known as ptyalin and is found in humans, the ape, pig, guinea pig, squirrel, mouse and rat. Salivary amylase plays a very important role in digestion of food that contains much starch but little sugar, such as rice and potato. The pancreatic amylase (α-amylase) hydrolyzes dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose as power source of energy for smooth f...
Amylase is exploited in textile, leather, wood, paper and detergent industries for desizing and w... more Amylase is exploited in textile, leather, wood, paper and detergent industries for desizing and washing of fabrics. Previously, it has been immobilized on to various compatible supports by several methods to increase its thermal and storage stability. Its immobilization onto bovine serum albumin (BSA) was found to be very significant to prepare its BSA loaded nanoparticles (BSANPs). In this study, Pearl millet amylase loaded jasmine oil driven BSA nanoparticles (BSANPs) were used and made bio-active with 35U of alkaline protease for their biodegradation. Then, these bio-active enzyme-BSANPs were used as nano-wool shampoo additives with chosen wool shampoo named, Wool & Cashmere Shampoo to test their stain removal efficacy from stained woolen fabric without affecting the delicacy and softness of the chosen fabric. In this study, chosen stains were comprised of daily routine stains of boot polish and hair color dye cream named, Kiwi Liquid boot polish Black and Loreal Paris Casting Crème Gloss Black respectively. Sometimes, these stains are very difficult to remove from the woolen fabric or required couple of pre-washing practices. So, keeping this domestic or industrial washing problem, selected stains are chosen very thoughtfully. And, from this study, an effective washing data was carried out to wash off the selected stains form woolen/cashmere/merino/mohair fabric when washed with chosen wool shampoo along with bio-active prepared jasmine oil driven amylase bound BSANPs solution as compared their washing with chosen wool shampoo alone.
Proteases are enzymes with highly specialized proteolytic functions. They are ubiquitous in occur... more Proteases are enzymes with highly specialized proteolytic functions. They are ubiquitous in occurrence, being found in all living organisms, and are essential for cell growth and differentiation. They not only have several physiological functions and roles in the living beings but are also of great importance in various industries as well thus providing a lot of economic benefits.
Alkaline phosphatase was isolated and purified from Solanum tuberosum (potato tubers) by a stream... more Alkaline phosphatase was isolated and purified from Solanum tuberosum (potato tubers) by a streamline method without the use of proteolytic and lipolytic enzymes and butanol. The study features are the use of a buffer solutions, ammonium sulphate precipitation at low temperature to remove impurity and the use of polyethylene glycol solution with potassium phosphate solution to concentrate the partially purified Solanum tuberosum , Colocasia esculenta (Taro), Punica granatum (pomegranate) and Phaseolus vulgaris (French beans) alkaline phosphatase by bi-phase extraction method. Enzyme activity of the extracted & purified Solanum tuberosum, Colocasia esculenta (Taro), Punica granatum (pomegranate) and Phaseolus vulgaris (French beans) alkaline phosphatase was done by para-nitro-phenyl-phosphate method (p-NPP method) and absorbance was taken at 410nm.
Alkaline phosphatise (ALP, ALKP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phos... more Alkaline phosphatise (ALP, ALKP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. This enzyme serves many important roles in human beings and also in various industries and therefore has a wide range of applications. This review aims to study this enzyme in detail laying special emphasis on it's mechanism, sources and industrial applications.
In present work, Cicer arietinum amylase was encapsulated by emulsification through covalent coup... more In present work, Cicer arietinum amylase was encapsulated by emulsification through covalent coupling by glutaraldehyde into chemically modified bovine serum albumin. Biodegradation of coconut oil driven emulsified bovine serum albumin encapsulated Cicer arietinum amylase was carried out by the alkaline protease for its controlled and sustained release of encapsulated enzyme from prepared microparticles of encapsulated Cicer arietinum amylase and its stability increased up to 6 months as compared to free enzyme. Its biodegradation was carried out by the using different concentration of alkaline protease (5U, 10U, 15U, 20U, 25U, 30U, 35U, 40U). Further, this coconut oil driven chemically modified bovine serum albumin microparticles of encapsulated Cicer arietinum amylase with alkaline protease were used with detergents for washing of stained cloths which have rust, gel pen ink, grease and chocolate strains. These chosen strains are very commonly present on uniforms of school going ch...
Amylase is known for starch saccharification and catalyses the starch hydrolysis producing glucos... more Amylase is known for starch saccharification and catalyses the starch hydrolysis producing glucose and limit dextrin which has wide range of applications in fermentation, textile, leather, detergents, food and paper industries. In the present work, amylase was extracted from Pennisetum glaucum (pearl millet) was entrapped in bovine serum albumin (BSA) nanoparticles in which emulsification was done by mustard oil and covalently coupled by using well known cross linking agents, glutaraldehyde. Bovine serum albumin is considered the most biodegradable, biocompatible and non-toxic biological matrix material for the preparation of enzyme bound nanospheres to enhance thermal stability, storage stability and suitability of bound enzymes as compared to free enzymes toward persisting ambient conditions. Formation of nanoparticles was confirmed by Dynamic Light scattering (DLS) and determination of exact size was observed by Scanning Electron Microscopy (SEM). Thermal stability of entrapped P...
Nanotechnology is the multidisciplinary emerging advanced technology that has vast array and pote... more Nanotechnology is the multidisciplinary emerging advanced technology that has vast array and potential to revolutionize the society
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
In present work, Cicer arietinum amylase was encapsulated into coconut driven emulsified bovine s... more In present work, Cicer arietinum amylase was encapsulated into coconut driven emulsified bovine serum albumin nanoparticle through glutaraldehyde. Biodegradation of coconut oil driven emulsified bovine serum albumin encapsulated Cicer arietinum amylase was carried out by using different units of alkaline protease (5U, 10U, 15U, 20U, 25U, 30U, 35U, 40U) to study their controlled and sustained release of encapsulated enzyme from prepared nanoparticles and hence, storage stability was enhanced up to 8 months as compared to free enzyme. Further, this coconut oil driven emulsified bovine serum albumin nanoparticles of encapsulated Cicer arietinum amylase with alkaline protease were used with detergents (aerial, surf excel, wheel and tide) for washing of stained cloth pieces which have coffee, tea, Pomegranate and turmeric stains. These chosen strains are very Common in our daily life and are very tough upon drying, hence, not to be easily washed off with well-known brand detergents upon ...
Amylase plays vital role in food, textile, leather, pharmaceutical and detergent industries due t... more Amylase plays vital role in food, textile, leather, pharmaceutical and detergent industries due to its efficacy and industrial viability. In our work, amylase was extracted from Pearl millet (Pennisetum glaucum) and encapsulated in bovine serum albumin nanoparticles by chemical modification with almond oil, n-butanol and glutaraldehyde. Hence, bovine serum albumin was found to be favourable matrix for encapsulating extracted amylase due to its well known exploitable properties e.g. biocompatibility, non-antigenicity and non-toxicity. Biodegradation of almond oil driven enzyme loaded bovine serum albumin nanoparticles was performed by using different units of alkaline protease () to study controlled release of encapsulated amylase. Kinetic parameters were also studied for Pearl millet extracted amylase and encapsulated amylase for their comparative analysis for optimal pH, incubation time, substrate concentration, CaCl 2 concentration and temperature for maximal enzyme activity which...
Targeted drug delivery is anadvanced method of delivering drugs to the patients in such a targete... more Targeted drug delivery is anadvanced method of delivering drugs to the patients in such a targeted sequences that increases the concentration of delivered drug to the targeted body part of interest only (organs/tissues/ cells) which in turn improves efficacy of treatment by reducing side effects of drug administration. Basically, targeted drug delivery is to assist the drug molecule to reach preferably to the desired site. The inherent advantage of this technique leads to administration of required drug with its reduced dose and reducedits side effect. This inherent advantage of targeted drug delivery system is under high consideration of research and development in clinical and pharmaceutical fields as backbone of therapeutics & diagnostics too. Various drug carrierwhich can be used in this advanced delivery system are soluble polymers, biodegradable microsphere polymers (synthetic and natural), neutrophils, fibroblasts, artificial cells, lipoproteins, liposomes, micelles and immune micelle. The goal of a targeted drug delivery system is to prolong, localize, target and have a protected drug interaction with the diseased tissue.
Amylases are enzymes which hydrolyzes starch as reserve carbohydrate in plants and glycogen as re... more Amylases are enzymes which hydrolyzes starch as reserve carbohydrate in plants and glycogen as reserve carbohydrate in animals into reducing fermentable sugars, mainly maltose, and reducing limit dextrins. Amylases are classified as saccharifying (β-amylase) and as dextrinizing (α-amylases). The α-amylases are found in all types of organs and tissues, whereas β-amylase is found almost exclusively in higher plants such as in seeds, cereals, yeasts, molds, and bacteria. The highest concentrations of amylase are found in the saliva and pancreas in animals. Salivary amylase is also known as ptyalin and is found in humans, the ape, pig, guinea pig, squirrel, mouse and rat. Salivary amylase plays a very important role in digestion of food that contains much starch but little sugar, such as rice and potato. The pancreatic amylase (α-amylase) hydrolyzes dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose as power source of energy for smooth f...
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Papers by Dr. Kirti Rani